BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
This work presents an implementation of an innovative single phase multilevel inverter using capacitors with reduced switches. The proposed Capacitor pattern H-bridge Multilevel Inverter (CPHMLI) topology consists of ...This work presents an implementation of an innovative single phase multilevel inverter using capacitors with reduced switches. The proposed Capacitor pattern H-bridge Multilevel Inverter (CPHMLI) topology consists of a proper number of Capacitor connected with switches and power sources. The advanced switching control supplied by Pulse Width Modulation (PDPWM) to attain mixed staircase switching state. The charging and discharging mode are achieved by calculating the voltage error at the load. Furthermore, to accomplish the higher voltage levels at the output with less number of semiconductors switches and simple commutation designed using CPHMLI topology. To prove the performance and effectiveness of the proposed approach, a set of experiments performed under various load conditions using MATLAB tool.展开更多
Total harmonic distortion (THD) is one of the key technical indexes of inverter output voltage. Optimization o f switching points and filter parameters could ensure the switching-point-preset sinusoidal pulse width mo...Total harmonic distortion (THD) is one of the key technical indexes of inverter output voltage. Optimization o f switching points and filter parameters could ensure the switching-point-preset sinusoidal pulse width modulation (SPWM) inverters with low harmonyic content in theory.The THD value would be increased by switching time delay of power devices and control circuit. A new control coecuit with delay time compensation is presented in this paper. With this control scheme, the output of the inverter could be basically identified with the theory given.Test results of experimental circuit verify that the control circuit presented in this paper is feasible. The THD of the inverter output voltage could be reduced to a certain extent by this method.展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
文摘This work presents an implementation of an innovative single phase multilevel inverter using capacitors with reduced switches. The proposed Capacitor pattern H-bridge Multilevel Inverter (CPHMLI) topology consists of a proper number of Capacitor connected with switches and power sources. The advanced switching control supplied by Pulse Width Modulation (PDPWM) to attain mixed staircase switching state. The charging and discharging mode are achieved by calculating the voltage error at the load. Furthermore, to accomplish the higher voltage levels at the output with less number of semiconductors switches and simple commutation designed using CPHMLI topology. To prove the performance and effectiveness of the proposed approach, a set of experiments performed under various load conditions using MATLAB tool.
文摘Total harmonic distortion (THD) is one of the key technical indexes of inverter output voltage. Optimization o f switching points and filter parameters could ensure the switching-point-preset sinusoidal pulse width modulation (SPWM) inverters with low harmonyic content in theory.The THD value would be increased by switching time delay of power devices and control circuit. A new control coecuit with delay time compensation is presented in this paper. With this control scheme, the output of the inverter could be basically identified with the theory given.Test results of experimental circuit verify that the control circuit presented in this paper is feasible. The THD of the inverter output voltage could be reduced to a certain extent by this method.
