Objective: Qi Gong is a mind-and-body intervention to improve health and relieve disease conditions,which has been practiced for thousands of years. Lack of quantitative measures to monitor the quality and progress of...Objective: Qi Gong is a mind-and-body intervention to improve health and relieve disease conditions,which has been practiced for thousands of years. Lack of quantitative measures to monitor the quality and progress of Qi Gong practice has hindered the high-quality study to evaluate its health benefits.Methods: To develop such quantitative measures, vital sign changes in Qi Gong meditation practice of a single individual were monitored for 5 years by wearable devices.Results: This retrospective and exploratory analysis revealed significant changes in blood oxygen saturation, pulse rate, respiration rate, and perfusion index in response to Qi Gong practice and physiological adaptation to its long-term training. Among the physiological changes in vital signs, a physiological state with a significant 2-fold increase in pulse-respiration rate ratio was detected during Qi Gong meditation while the ratios were maintained at 4 under resting, sleeping, and mild cycling conditions.Conclusion: This is an inaugural report of a five-year longitudinal observation of this kind. Once validated in a well-designed cohort study, these non-invasive and easily obtained vital signs may be used as biomarkers to assess the adherence to breathing control during Qi Gong practice and can be applied to quantify the quality of Qi Gong practice in clinical trials with Qi Gong intervention.展开更多
Objective:To explore the influence of centrifugal parameters,centrifugal temperature,preparation equipment and anticoagulant on the manual preparation of platelet-rich plasma(PRP).Methods:Female ICR mice(n=36)were div...Objective:To explore the influence of centrifugal parameters,centrifugal temperature,preparation equipment and anticoagulant on the manual preparation of platelet-rich plasma(PRP).Methods:Female ICR mice(n=36)were divided into 4 groups according to four influencing factors of centrifugation parameters(centrifugation force and centrifugation time),centrifugation temperature,preparation device,and anticoagulant.The red blood cell concentration(RBC),white blood cell concentration(WBC)and platelet concentration(PLT)were detected by blood cell analyzer,and the platelet enrichment factor(PEF)and platelet recovery ratio(PRR)were calculated.Results:In the comparison of the calculation results of PLT,PEF and PRR in each group,the centrifugal parameters were higher at 300 g for 10 min and 1200 g for 15 min,which were 2600±201.55,3.68±1.29,61.60%±8.57%(P<0.05);When the centrifugal temperature was 4℃,the tunica albuginea was flatter and easier to be absorbed than the normal temperature,and the RBCs between the groups were 0.84±0.33 and 3.16±0.33,respectively,with significant difference.There was no significant difference among the three data groups of anticoagulants,and the choice of anticoagulants did not have too much influence.The quality of PRP obtained from the improved preparation device(anticoagulant+syringe)was significantly higher than that of the anticoagulant tube,and the results were 2568±124.52 and 3551.67±348.12,3.31±0.16 and 4.58±0.45,49.7%±2.41%and 68.74%±6.74%,respectively.Conclusion:High-quality platelet-rich plasma can be obtained by using an improved extraction device with any anticoagulant at 4℃and centrifugation parameters of 300 g for 10 min and 1200 g for 15 min.展开更多
文摘Objective: Qi Gong is a mind-and-body intervention to improve health and relieve disease conditions,which has been practiced for thousands of years. Lack of quantitative measures to monitor the quality and progress of Qi Gong practice has hindered the high-quality study to evaluate its health benefits.Methods: To develop such quantitative measures, vital sign changes in Qi Gong meditation practice of a single individual were monitored for 5 years by wearable devices.Results: This retrospective and exploratory analysis revealed significant changes in blood oxygen saturation, pulse rate, respiration rate, and perfusion index in response to Qi Gong practice and physiological adaptation to its long-term training. Among the physiological changes in vital signs, a physiological state with a significant 2-fold increase in pulse-respiration rate ratio was detected during Qi Gong meditation while the ratios were maintained at 4 under resting, sleeping, and mild cycling conditions.Conclusion: This is an inaugural report of a five-year longitudinal observation of this kind. Once validated in a well-designed cohort study, these non-invasive and easily obtained vital signs may be used as biomarkers to assess the adherence to breathing control during Qi Gong practice and can be applied to quantify the quality of Qi Gong practice in clinical trials with Qi Gong intervention.
文摘Objective:To explore the influence of centrifugal parameters,centrifugal temperature,preparation equipment and anticoagulant on the manual preparation of platelet-rich plasma(PRP).Methods:Female ICR mice(n=36)were divided into 4 groups according to four influencing factors of centrifugation parameters(centrifugation force and centrifugation time),centrifugation temperature,preparation device,and anticoagulant.The red blood cell concentration(RBC),white blood cell concentration(WBC)and platelet concentration(PLT)were detected by blood cell analyzer,and the platelet enrichment factor(PEF)and platelet recovery ratio(PRR)were calculated.Results:In the comparison of the calculation results of PLT,PEF and PRR in each group,the centrifugal parameters were higher at 300 g for 10 min and 1200 g for 15 min,which were 2600±201.55,3.68±1.29,61.60%±8.57%(P<0.05);When the centrifugal temperature was 4℃,the tunica albuginea was flatter and easier to be absorbed than the normal temperature,and the RBCs between the groups were 0.84±0.33 and 3.16±0.33,respectively,with significant difference.There was no significant difference among the three data groups of anticoagulants,and the choice of anticoagulants did not have too much influence.The quality of PRP obtained from the improved preparation device(anticoagulant+syringe)was significantly higher than that of the anticoagulant tube,and the results were 2568±124.52 and 3551.67±348.12,3.31±0.16 and 4.58±0.45,49.7%±2.41%and 68.74%±6.74%,respectively.Conclusion:High-quality platelet-rich plasma can be obtained by using an improved extraction device with any anticoagulant at 4℃and centrifugation parameters of 300 g for 10 min and 1200 g for 15 min.
