Posttranscriptional regulation of cancer gene expression programs plays a vital role in carcinogenesis;identifying the critical regulators of tumorigenesis and their molecular targets may provide novel strategies for ...Posttranscriptional regulation of cancer gene expression programs plays a vital role in carcinogenesis;identifying the critical regulators of tumorigenesis and their molecular targets may provide novel strategies for cancer diagnosis and therapeutics.Highly conserved RNA-binding protein Pumilio-1(PUM1)regulates mouse growth and cell proliferation,propelling us to examine its role in cancer.We found human PUM1 is highly expressed in a diverse group of cancer,including prostate cancer;enhanced PUM1 expression is also correlated with reduced survival among prostate cancer patients.Detailed expression analysis in twenty prostate cancer tissues showed enhanced expression of PUM1 at mRNA and protein levels.Knockdown of PUM1 reduced prostate cancer cell proliferation and colony formation,and subcutaneous injection of PUM1 knockdown cells led to reduced tumor size.Downregulation of PUM1 in prostate cancer cells consistently elevated cyclin-dependent kinase inhibitor 1B(CDKN1B)protein expression through increased translation but did not impact its mRNA level,while overexpression of PUM1 reduced CDKN1B protein level.Our finding established a critical role of PUM1 mediated translational control,particularly the PUM1-CDKN1B axis,in prostate cancer cell growth and tumorigenesis.We proposed that PUM1-CDKN1B regulatory axis may represent a novel mechanism for the loss of CDKN1B protein expression in diverse cancers and potential targets for therapeutics development.展开更多
通过子带Wiener滤波结合PUM(Probabilistic Union Model)模型,实现在噪声环境下连续字语音识别的方法。该方法先通过对语音信号进行子带Wiener滤波预处理消除已知噪声,为PUM模型提供只有局部被噪声污染的语音信号,再利用PUM模型进行抗...通过子带Wiener滤波结合PUM(Probabilistic Union Model)模型,实现在噪声环境下连续字语音识别的方法。该方法先通过对语音信号进行子带Wiener滤波预处理消除已知噪声,为PUM模型提供只有局部被噪声污染的语音信号,再利用PUM模型进行抗噪语音识别。试验表明在各种不同的噪声环境下,该新方法有更高的平均识别率。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.31771652 and No.81270737).
文摘Posttranscriptional regulation of cancer gene expression programs plays a vital role in carcinogenesis;identifying the critical regulators of tumorigenesis and their molecular targets may provide novel strategies for cancer diagnosis and therapeutics.Highly conserved RNA-binding protein Pumilio-1(PUM1)regulates mouse growth and cell proliferation,propelling us to examine its role in cancer.We found human PUM1 is highly expressed in a diverse group of cancer,including prostate cancer;enhanced PUM1 expression is also correlated with reduced survival among prostate cancer patients.Detailed expression analysis in twenty prostate cancer tissues showed enhanced expression of PUM1 at mRNA and protein levels.Knockdown of PUM1 reduced prostate cancer cell proliferation and colony formation,and subcutaneous injection of PUM1 knockdown cells led to reduced tumor size.Downregulation of PUM1 in prostate cancer cells consistently elevated cyclin-dependent kinase inhibitor 1B(CDKN1B)protein expression through increased translation but did not impact its mRNA level,while overexpression of PUM1 reduced CDKN1B protein level.Our finding established a critical role of PUM1 mediated translational control,particularly the PUM1-CDKN1B axis,in prostate cancer cell growth and tumorigenesis.We proposed that PUM1-CDKN1B regulatory axis may represent a novel mechanism for the loss of CDKN1B protein expression in diverse cancers and potential targets for therapeutics development.
文摘通过子带Wiener滤波结合PUM(Probabilistic Union Model)模型,实现在噪声环境下连续字语音识别的方法。该方法先通过对语音信号进行子带Wiener滤波预处理消除已知噪声,为PUM模型提供只有局部被噪声污染的语音信号,再利用PUM模型进行抗噪语音识别。试验表明在各种不同的噪声环境下,该新方法有更高的平均识别率。
