Impact of Inulin Extracted, Purified from (Chicory and Globe Artichoke) Roots and the Combination with Maltodextrin as Prebiotic Dietary Fiber on the Functional Properties of Stirred Bio-Yogurt

Inulin is a prebiotic dietary fiber that plays an integral role in producing functional dairy products with improved health benefits. Therefore, the objectives of this study are as follows: extract and purify inulin from chicory roots and globe artichoke roots;evaluate the physicochemical, functional properties and functional groups of the purified inulin;determine the functional properties of chicory roots inulin-maltodextrin and globe artichoke roots inulin-maltodextrin and compare it with that of the commercial inulin;examine the impact of various inulin on physiochemical, microstructural, textural, sensory characteristics and as prebiotic dietary fiber on probiotic bacteria’s viability of stirred bio-yogurt. The characteristics of the microstructure were investigated by scanning electron microscopy and, Fourier transforms infrared spectroscopy to detect the functional group. The resulting inulin exhibited a high yield and purity along with enhanced functional properties. Stirred bio-yogurt fortified with chicory roots inulin or globe artichoke roots inulin showed enhanced physicochemical, microstructural, microbiological, and overall sensorial acceptability followed by chicory roots inulin-maltodextrin or globe artichoke roots inulin-maltodextrin and the commercial inulin as compared to the control. Stirred bio-yogurt samples can offer various health benefits and wide applications as supplement of prebiotic dietary fiber in dairy industry.

菊苣多糖对四氧嘧啶诱导糖尿病小鼠的影响

[目的]探究菊苣多糖对糖尿病(DM)模型小鼠肝脏氧化应激的缓解作用。[方法]采用乙醇提取法从菊苣种子中提取多糖,采用苯酚-硫酸法测定多糖,并测试DPPH自由基清除作用。采用四氧嘧啶复制小鼠DM模型,取48只DM小鼠随机分组为四氧嘧啶模型组(ALX)、盐酸二甲双胍阳性组(ALX+M)、菊苣多糖低剂量组(ALX+L)、菊苣多糖高剂量组(ALX+H),每组12只;另取12只健康小鼠作为正常对照组(NC);每日灌胃给药1次,连续给药15 d。末次给药后,采集血清测定谷丙转氨酶(ALT)、谷草转氨酶(AST)、血糖、肌酐以及小鼠肝组织氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT),观察小鼠肝、肾、胰腺、睾丸等组织病理变化。[结果]菊苣种子多糖提取率为39%,具有一定的清除DPPH自由基作用;四氧嘧啶能引起小鼠血清ALT、AST、血糖、肌酐指标上升(P<0.01),肝脏、肾脏组织发生肝细胞排列紊乱、肝小叶结构破坏、肾小球萎缩、间质增大等病理变化。菊苣多糖高剂量组(ALX+H)具有明显改善小鼠体重、脏器指数,缓解肝组织GSH、SOD、CAT水平(P<0.05),明显改善肝组织形态结构,睾丸组织病理学变化趋向NC组。[结论]菊苣多糖具有一定的抗氧化应激作用。

菌株JB18的鉴定及对菊苣幼苗生理指标的影响

铅镉污染严重危害植物生长与人类健康.筛选了一株铅镉抗性细菌JB18,进行了16SrDNA序列分析鉴定.用不同铅镉复合质量浓度处理土壤并种植菊苣,探究JB18对菊苣幼苗生理指标的影响.测定了菊苣的可溶性糖、可溶性蛋白、谷胱甘肽、脯氨酸的质量分数变化.结果表明,经鉴定菌株JB18为耐辐射甲基杆菌(Methylobacterium radiotolerans),其最大耐铅镉质量浓度为Pb-Cd(200/40)mg·L^(-1),能够水解淀粉,利用麦芽糖.盆栽实验表明,接种JB18后,可提升菊苣幼苗的可溶性糖、可溶性蛋白、脯氨酸等物质的质量分数,同时可促进谷胱甘肽的合成,减缓铅镉胁迫对菊苣幼苗的不利影响.

不同施肥量和种植密度对紫锥菊质量的影响

[目的]探究紫锥菊(Echinacea purpurea(L.)Moench)在陕西省凤县的应用潜力及栽培技术。[方法]于2022-2023年开展大田试验,采用二裂式裂区试验设计,以施肥量为主区,共设5个处理F0~F4(施肥量依次为酵母源烟茎生物有机肥:0、1200、2100、3000、3900 kg·hm^(-2);酵母源有机无机复混肥:0、360、630、900、1170 kg·hm^(-2));移栽密度为裂区,设置2个水平,D1(63000窝·hm^(-2))和D2(31500窝·hm^(-2)),平均每窝种植3株,分别在2022年10月、2023年7月和2023年10月采样,测定不同处理紫锥菊株高、茎粗、开花枝条数、叶绿素含量、地上部分干重、根干重等生物学性状,以及地上部分和地下部分菊苣酸、单咖啡酰酒石酸、黄酮、多糖含量和紫锥菊药材产量等指标。[结果]在同一施肥条件下,随着种植密度的增加,紫锥菊的各生物学性状指标降低,除多糖含量以外其它有效成分含量及药材产量随种植密度的增加而增加;在同一栽培密度下,随着施肥量的增加,各生物学性状指标、有效成分含量及药材产量呈现先升高后降低的趋势。施肥量、种植密度以及施肥量和种植密度的互作效应对紫锥菊菊苣酸、单咖啡酰酒石酸、黄酮、多糖含量和紫锥菊药材产量有显著影响(P<0.05)。紫锥菊地上部分菊苣酸、单咖啡酰酒石酸、黄酮含量高于地下部分,而地下部分多糖含量高于地上部分,并且以2023年7月采收的紫锥菊质量及产量最佳。其中F1D1、F2D1处理组有较高的产量及有效成分含量。[结论]推荐紫锥菊种植时施1200~2100kg·hm^(-2)酵母源烟茎生物有机肥和360~630kg·hm^(-2)酵母源有机无机复混肥,种植密度为每公顷63000窝(种植株距为30 cm,行距为60 cm),以获得较高品质和产量的紫锥菊药材。

互花米草复合咀嚼片的研究与开发

互花米草中的活性成分具有降尿酸、抗痛风的作用。由于互花米草粉单独使用具有较强烈的咸味,以互花米草粉为原料,添加菊苣根粉、菊粉、甘露醇进行复配,采用粉末直接压片法,先确立基础配方,并进行配方优化,以感官评价、硬度、脆碎度、颗粒产率为综合指标,制备一种对降尿酸有作用且口感佳的咀嚼片。通过试验得到互花米草复合咀嚼片的最优配方为互花米草粉用量3.5 g,菊苣根粉用量5 g,甘露醇用量13 g,菊粉用量11 g。利用该配方得到的互花米草复合咀嚼片的综合评价最好。

HPLC法测定乳苣不同部位菊苣酸含量

采用超声法提取乳苣不同部位总多酚,建立了测定乳苣不同部位菊苣酸含量的HPLC法,色谱条件为:岛津LC-16型高效液相色谱仪,SPD检测器,C18色谱柱(4.6 mm×150 mm,5μm),柱温为40℃,流动相为甲醇-0.1%磷酸,梯度洗脱,流速为1.0 mL·min^(-1),检测波长为280 nm,进样量为10μL。结果表明,菊苣酸浓度在11.6~58.0μg·mL^(-1)范围内与峰面积线性关系良好(R^(2)=0.9994),平均加标回收率为95.30%(n=6);乳苣根、茎、叶、全草中菊苣酸含量分别为23.70 mg·g^(-1)、37.20 mg·g^(-1)、114.24 mg·g^(-1)、102.50 mg·g^(-1),乳苣不同部位菊苣酸含量大小顺序为:叶>全草>茎>根。该方法简便、快速、准确,适用于乳苣中菊苣酸含量的测定。

菊苣酸通过调控Nrf2/HO-1对糖尿病肾病氧化应激的抑制作用

目的探索菊苣酸(CA)对糖尿病肾病(DN)小鼠氧化应激的作用及其可能机制。方法给予C57BL/6小鼠高脂饮食,同时注射链脲霉素建立DN小鼠模型,并使用20 mmol/L的D-葡萄糖刺激NRK-52E细胞建立体外模型。ELISA检测丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)水平。对肾组织进行HE染色、Masson染色和PAS染色,Western blot检测Nrf2和HO-1蛋白水平。结果CA能减少肾重指数,改善肾小球结构损伤,改善摄取水量、尿蛋白水平、血清肌酐和尿素氮水平。在DN小鼠模型和体外模型中,CA均能降低肾组织和细胞中的MDA水平,增加SOD、GSH和GSH-PX水平。CA还激活DN小鼠肾脏组织和体外模型肾细胞中的Nrf2/HO-1信号通路。结论CA通过激活Nrf2/HO-1信号通路,减少DN及其氧化应激损伤。CA可能是一种治疗或预防DN的有希望的治疗药物。

Chicory(Cichorium intybus L.) and cereals differently affect gut development in broiler chickens and young pigs

Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastrics. Furthermore, it can be used as in-feed antibiotic alternative. Chicory （Cichorium intybus L.） forage with high content of pectin （uronic acids as building blocks） is a novel class of dietary fiber that is chemically different from cereal grains （with high content of arabinoxylans）. In the present study, we investigated effects of dietary inclusion of chicory forage on digestibility, gut morphology and microbiota in broilers and young pigs. In the chicken experiment, 160 1-d old broiler chicks were fed 3 nutritionally balanced diets for 30 d including a cereal-based diet and 2 diets with part of the cereals substituted with 60 and 120 g/k9 chicory forage （CF60 and CF120], whereas in the pig experiment, 18 seven-wk old Yorkshire pigs were fed 3 diets for 18 d including a cereal-based diet and 2 diets with 80 and 160 g/kg chicory forage inclusion （CF80 and CF160）. Our results showed that young pigs were capable to utilize chicory forage well with higher total tract apparent digestibility （TTAD） of all fiber fractions, particularly uronic acid, compared with the control （P 〈 0.01）. In contrast, a decreased TTAD of all fiber fractions was observed in chickens fed on diet CF120 （P 〈 0.05）. Moreover, diet induced changes in gut morphology were observed in the large intestine of chickens. The alteration of cecal mucosal thickness was further positively correlated with TTAD of non-starch polysaccharides （NSP） and its constituent sugars （P 〈 0.05）. In addition, in pigs, terminal restriction fragment length polymorphism （T-RFLP） analysis of intestinal microbiota revealed substantial dietary effects （cereal control diet vs. chicory forage inclusion） on the relative abundance of 2 dominant bacterial phylotypes （Prevotella sp. vs. Roseburia sp.） respectively （P 〈 0.05）. In conclusion, our data showed that chicory forage （Cichorium intybus L.）, a novel dietary fiber source in animal nutrition, have potential beneficial properties as fiber ingredient in diets for both pigs and chickens.

Chicory (Cichorium intybus L.) inhibits renal reabsorption by regulating expression of urate transporters in fructose-induced hyperuricemia

Objective:Hyperuricemia is an excess of urate in blood.The kidneys play important parts in urate excretion,which involves handling reabsorption and secretion.A series of urate transporters is responsible for this process:urate transporter (URAT)1,glucose transporter (GLUT)9,organic anion transporter (OAT)1 and OAT3.Excessive fructose intake may result in increased serum urate levels.Chicory (Cichorium intybus L.) has been used as an edible vegetable and traditional Chinese medicine.Studies have shown that chicory is a promising anti-hyperuricemia agent and we explored the mechanism of its uricosuric effect via a renal pathway.Methods:Hyperuricemia was induced in rats by administration of 10% fructose.The uricosuric effect was evaluated by determining the serum urate level.Renal excretory function was detected by the clearance rate of creatinine,clearance rate of uric acid and histology.The location and expression of URAT1,GLUT9,OAT1 and OAT3 their mRNA expression in kidneys were analyzed.Results:Chicory decreased serum levels of urate and creatinine significantly,and promoted the clearance of creatinine and urate,as well as improving renal pathologic changes due to hyperuricemia.Chicory inhibited expression of URAT1 and GLUT9 markedly in a dosedependent manner,but showed no influence on expression of OAT1 or OAT3.Conclusion:Chicory might be a promising anti-hyperuricemia agent.It can promote renal excretion of urate by inhibiting urate reabsorption,which may be related to downregulation of mRNA and protein expression of URAT1 and GLUT9.

Chicory polysaccharides alleviate high-fat diet-induced non-alcoholic fatty liver disease via alteration of lipid metabolism-and inflammation-related gene expression

Administration of chicory polysaccharides(CP) can modify lipid metabolism, improve dyslipidemia, and reduce liver inflammation, all of which may help alleviate non-alcoholic fatty liver disease(NAFLD). However, the underlying mechanisms remain unclear. This study aimed to gain further understanding of the potential molecular mechanisms that mediate the protective effects of CP against NAFLD via transcriptomic and metabolomic analyses. Hepatic RNA-sequencing analysis demonstrated that long-term intake of CP restored expression of lipid metabolism-related genes Xbp1, Insig2, and Cth in NAFLD rats, thereby inhibiting de novo lipogenesis. Moreover, CP consumption restored expression levels of genes involved in pro-inflammatory responses, such as Irf1. In addition, metabolic data confirmed that CP treatment increased levels of L-palmitoylcarnitine and hexadecanoyl-Co A, implying that CP administration can promote hepatic fatty acid β-oxidation. The present results demonstrate the underlying mechanisms of CP in high-fat dietinduced NAFLD rats and suggest that CP treatment might provide a dietary therapeutic tool for the treatment of NAFLD in humans.

Fecal microbiome of growing pigs fed a cereal based diet including chicory(Cichorium intybus L.) or ribwort(Plantago lanceolata L.) forage

Background： The purpose of this study was to investigate how inclusion of chicory forage or ribwort forage in a cereal-based diet influenced the fecal microbial community（microbiome） in newly weaned（35 days of age） piglets.The piglets were fed a cereal-based diet without（B） and with inclusion（80 and 160 g/kg air-dry forage） of vegetative shoots of chicory（C） and leaves of ribwort（R） forage in a 35-day growth trial. Fecal samples were collected at the start（D0）, 17（D17） and 35（D35） days after weaning and profiles of the microbial consortia were generated using terminal restriction fragment length polymorphism（T-RFLP）. 454-FLX pyrosequencing of 16 S r RNA gene amplicons was used to analyze the microbial composition in a subset of the samples already analyzed with T-RFLP.Results： The microbial clustering pattern was primarily dependent on age of the pigs, but diet effects could also be observed. Lactobacilli and enterobacteria were more abundant at D0, whereas the genera Streptococcus, Treponema,Clostridium, Clostridiaceae1 and Coprococcus were present in higher abundances at D35. Pigs fed ribwort had an increased abundance of sequences classified as Treponema and a reduction in lactobacilli. However, the abundance of Prevotellaceae increased with age in on both the chicory and the ribwort diet. Moreover, there were significant correlations between the abundance of Bacteroides and the digested amount of galactose, uronic acids and total non-starch polysaccharides, and between the abundance of Bacteroidales and the digested amount of xylose.Conclusion： This study demonstrated that both chicory and ribwort inclusion in the diet of newly weaned pigs influenced the composition of the fecal microbiota and that digestion of specific dietary components was correlated with species composition of the microbiota. Moreover, this study showed that the gut will be exposed to a dramatic shift in the microbial community structure several weeks after weaning.

Effects of chicory(Cichorium intybus L.)on nonalcoholic fatty liver disease

There is a dramatic increase in the prevalence of nonalcoholic fatty liver disease, which is slowly turning into apandemic as well as a major challenge across the world. Nonalcoholic fatty liver disease is described as a range ofliver conditions such as fat accumulation, hepatic steatosis, or end-stage liver disease. Patients with nonalcoholicfatty liver disease are asymptomatic and their mortality is higher than people without nonalcoholic fatty liverdisease. The pathogenesis of nonalcoholic fatty liver disease has not been clearly determined yet. The “two hits”hypothesis is designed to explain the pathogenesis of nonalcoholic fatty liver disease. Dyslipidemia, oxidativestress, insulin resistance, obesity, and chronic inflammation are some of the morbidities involved in the progressionof nonalcoholic fatty liver disease. Chicory (Cichorium intybus L.) is an herbaceous perennial, known as chicory.Chicory contains various compounds, such as vitamins, sonchuside A, caffeic acid derivatives,fructo-oligosaccharides, chlorogenic acid, magnolialide, polysaccharides, coumarins, phenolic acids, terpenoids,flavonoids, polyphenol, cichoriosides, ixerisosides, eudesmanolides, inulin, bitter sesquiterpene lactones, andalkaloids. Current research has revealed that chicory supplementation might be effective in the treatment ofnonalcoholic fatty liver disease. The anti-inflammatory, antihepatotoxic, antihyperlipidemic, antidiabetic,antihyperglycemic, and antioxidant properties of chicory provide plausible mechanisms by which chicory mayaffect the various steps of disease progression and severity. Existing studies have shown that chicorysupplementation has beneficial effects on nonalcoholic fatty liver disease, but the existence of only one humanstudy and possible side effects of chicory necessitate further studies.

菌株JB12影响铅镉胁迫下菊苣黄酮合成的转录组分析

为探究菌株JB12对菊苣(Cichorium intybus)幼苗铅镉抗性和黄酮生物合成的影响,将菌株JB12接种于铅镉复合(Pb-Cd)浓度为400~40 mg·kg^(-1)的土壤中,测定幼苗生长指标、铅镉含量、抗氧化酶活性、总黄酮含量和黄酮生物合成相关酶活性,并对叶片转录组数据进行分析。结果表明,接种菌株JB12后,叶片过氧化氢(H 2O 2)、丙二醛(Malondialdehyde,MDA)及地上部和根部铅镉含量均低于胁迫处理组,植株干重、抗氧化酶活性、总黄酮含量及黄酮生物合成相关酶活性均高于胁迫处理组。对叶片转录组数据和RT-qPCR验证分析表明,苯丙素和黄酮生物合成途径相关基因表达上调可能是菌株JB12提高菊苣幼苗铅镉抗性的重要机制。综上所述,菌株JB12在减少菊苣幼苗铅镉积累的同时,还可以通过增强抗氧化酶活性及调控叶片黄酮生物合成相关基因的表达,提高菊苣幼苗的铅镉抗性和总黄酮的积累。

菊苣复合物30 d喂养实验对SD大鼠生理状况的影响

目的:分析了解在30 d喂养实验中使用菊苣复合物对SD大鼠进行喂养并观察SD大鼠的生理状况变化。方法:选取60只体型相似的SD大鼠进行为期30 d的喂养实验,将60只大鼠每30只分为一组,雌雄各半,分为观察组和对照组。观察组采用菊苣复合物对大鼠进行喂养,对照组则采用传统喂养方式对大鼠进行喂养,观察并记录菊苣复合物对大鼠生理状况的影响。结果:在30 d动物喂养期间,菊苣复合物未引起大鼠的生理、生化功能器官以及组织形态、整体健康状况等身体的各项指标的异常变化,各项检查结果对照组传统鼠粮与菊苣复合物喂养相比较,差异均无统计学意义。结论:30 d大鼠喂养实验后,对比喂养前前后以及观察组的数据分析食用菊苣复合物对大鼠的各项生理指标有明显的改善,所以菊苣复合物喂养SD大鼠对其的生理指标有益。

菊苣、陈皮、鸡内金、山楂和余甘子对胃动力障碍大鼠的改善作用研究

该文采用大鼠胃动力障碍模型,研究了菊苣、陈皮、鸡内金、山楂和余甘子等5种药食同源食物的促胃动力效果,主要测定了胃排空率、胃酸、蛋白酶、胃液、血清饥饿素、胃泌素、酪氨基酶受体基因(c-kit)和干细胞因子的转录水平等指标,观察胃窦组织切片。结果发现菊苣、山楂和余甘子能显著提高胃排空速率(P<0.05),菊苣和陈皮显著降低胃游离酸的浓度和排出速率(P<0.05),山楂显著提高胃蛋白酶活性和排出量(P<0.05),鸡内金显著提高了胃蛋白酶排出速率和胃液量(P<0.05)。另外,菊苣、山楂显著提高血清饥饿素含量(P<0.05),余甘子显著提高血清胃泌素含量(P<0.05),菊苣对胃底腺层有修复作用并能显著上调c-kit的转录水平。综上,菊苣、山楂和余甘子对胃动力的促进效果较好,具有开发成促消化食品的潜力。

菊苣对不同性别麻黄肉鸡生长性能、屠宰性能和肉品质的影响

【目的】研究菊苣对不同性别麻黄肉鸡生长性能、屠宰性能及肉品质的影响。【方法】选取42日龄麻黄肉鸡576只,随机分为8组,每组6个重复,每个重复12只鸡,公母各半。采用2(性别)×4(菊苣水平)双因素试验设计,菊苣添加水平为0、3%、6%、9%。试验期42 d。试验结束时统计生产性能指标;每重复屠宰1只鸡,测定屠宰性能和免疫器官指数;取胸肌和腿肌用于肉质分析。【结果】①与不添加菊苣相比,添加6%和9%菊苣可显著提高麻黄肉鸡的料重比(F/G)(P<0.05);公鸡生长性能显著优于母鸡(P<0.05)。②母鸡的法氏囊指数显著高于公鸡(P<0.05)。③公鸡的全净膛率显著高于母鸡(P<0.05),腹脂率显著低于母鸡(P<0.05)。④与不添加菊苣相比,添加6%和9%菊苣可显著提高麻黄肉鸡胸肌的黄度(b_(24 h)^(*))(P<0.05),母鸡胸肌的亮度(L_(24 h)^(*))显著高于公鸡(P<0.05)。⑤性别和菊苣水平对麻黄肉鸡腿肌的剪切力存在显著交互作用(P<0.05),与不添加菊苣相比,添加6%和9%菊苣可显著降低公鸡腿肌的剪切力(P<0.05)。公鸡腿肌的红度(a_(45 min)^(*)、a_(24 h)^(*))和b_(45 min)^(*)显著高于母鸡(P<0.05),L_(45 min)^(*)和L_(24 h)^(*)显著低于母鸡(P<0.05)。性别和菊苣水平对麻黄肉鸡的生长性能、免疫器官指数、屠宰性能和胸肌肉品质均无显著交互作用(P>0.05)。【结论】公鸡的生长性能、屠宰性能和肌肉品质均优于母鸡;添加6%和9%菊苣虽可改善麻黄肉鸡的肉品质,但对F/G有不利影响。综合各项指标评定,菊苣最适添加量为3%。

菊苣酸对低氧诱导下的H9c2大鼠心肌细胞氧化损伤水平、炎性因子、能量代谢的影响

试验旨在研究低氧诱导下菊苣酸(CA)对H9c2大鼠心肌细胞氧化损伤水平、炎性因子、能量代谢的影响。试验以H9c2大鼠心肌细胞为研究对象,分为对照组(常氧组)、低氧组(1%氧浓度)和低氧(1%氧浓度)+CA组(60 mg/L),每组3个重复。结果表明,与对照组相比,低氧组细胞过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性、白细胞介素10(IL-10)含量及能量代谢因子Ca^(2+)ATP酶(Ca^(2+)-ATPase)、Na^(+)K^(+)-ATP酶(Na^(+)K^(+)-ATPase)活性极显著降低(P<0.01);低氧组丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量极显著升高(P<0.01);低氧组细胞血红素氧化酶-1(HO-1)含量显著降低(P<0.05)。与低氧组相比,低氧+CA组细胞GSH-Px、Ca^(2+)ATP酶、Na^(+)K^(+)-ATP酶活性及HO-1含量极显著升高(P<0.01),SOD活性显著升高(P<0.05),MDA、TNF-α、IL-6含量极显著降低(P<0.01)。研究表明,CA可通过提高低氧诱导下的H9c2大鼠心肌细胞的抗氧化能力,减少促炎因子的分泌,增加抗炎因子的分泌,改善能量代谢水平,从而缓解低氧诱导下造成的H9c2大鼠心肌细胞的氧化损伤。

菊苣提取物抗阿霉素心脏毒性作用挖掘及验证

目的挖掘并验证菊苣提取物抗阿霉素心脏毒性的潜在作用。方法进行文献研究,整合菊苣抗心肌损伤的研究进展;基于网络药理学探讨菊苣抗心肌损伤机制,分析其与阿霉素心脏毒性机制的重合性;采用15 mg·kg-1阿霉素单次腹腔注射建立心脏毒性小鼠模型,给予低、中、高剂量菊苣水提物(3.8、19、38 g·kg-1)干预8 d,验证菊苣抗阿霉素心脏毒性的潜在作用。结果在菊苣抗心肌损伤领域共有13篇文章,均为基础研究,包括菊苣醇提物、菊苣酸以及菊苣多糖等药物,包括纳米铅、脓毒症致心肌损伤等动物模型;菊苣心脏保护作用机制主要为抗炎抗氧化应激,涉及ErbB、MAPK、PI3K-Akt等信号通路及TP53、Src、STAT3、AKT1等核心靶点,与阿霉素心脏毒性发病机制有较大重合性;菊苣水提物可显著升高模型小鼠心脏系数及终末体重,降低模型小鼠心脏组织匀浆及血清NT-ProBNP、CKMB、LDH、AST水平,改善模型小鼠心脏组织纤维排列紊乱,核固缩等情况。结论菊苣具有抗阿霉素心脏毒性的潜在作用,值得进一步挖掘与研究,以指导阿霉素及菊苣的临床合理应用。

基于产品开发的菊苣生物活性研究进展

菊苣为菊科菊苣属多年生草本植物。菊苣中含多糖类、萜类、酚酸类、黄酮类、香豆素类等多种成分,为药食两用植物。作为中药材,菊苣具有清热解毒,健胃消食,利尿消肿等功效,收录于《中国药典》2020年版。本文通过查阅近年国内外相关文献,对菊苣化学成分、生物活性研究进展和专利申请情况进行综述,以期为菊苣的合理开发和应用提供依据。