Bacteriorhodopsin is a well-known photoresponsive protein in the cell membrane of Halobacterium salinarum and exists in the patch called the purple membrane(PM).This letter reports,for the first time,its new function ...Bacteriorhodopsin is a well-known photoresponsive protein in the cell membrane of Halobacterium salinarum and exists in the patch called the purple membrane(PM).This letter reports,for the first time,its new function as a natural non-fouling substance for resisting cell adhesion.Mammalian cells such as murine preosteocytes MC3T3-E1 were seeded on the PM film.A significant resistance to cell adhesion on the film was found.展开更多
Purple membrane, molecular weight 26ku, is synthesized by Holobacterium halobium on some parts of its cell membrane under a special condition combined by 248 residues,and 7 times acrossing membrane. Only one kind of p...Purple membrane, molecular weight 26ku, is synthesized by Holobacterium halobium on some parts of its cell membrane under a special condition combined by 248 residues,and 7 times acrossing membrane. Only one kind of protein, bacteriorhodopsin (BR), is on the membrane. After light excitation, BR goes through a series of intermediate processes K, L, M, N, O and recovers to BR. Purple membrane is an ideal biomaterial for photo energy transfer, and becomes a new focus field of many branches of science. Because the photocycle of BR is related to light-driven proton pump and photoelectric response,展开更多
The purple membrane (PM) is found on the plasma membrane of Halobacteria Halobium,which contains an integral membrane protein, bacteriorhodopsin (BR). The structure andfunction of bacteriorhodopsin are rather clear at...The purple membrane (PM) is found on the plasma membrane of Halobacteria Halobium,which contains an integral membrane protein, bacteriorhodopsin (BR). The structure andfunction of bacteriorhodopsin are rather clear at present. Protein to lipid ratio of the purplemembrane is 1:10 (mol/mol). Most of lipids of purple membranes are negatively charged atneutral pH.展开更多
Bacteriorhodopsin is the only protein in purple membrane. When illuminated, optically distinct intermediates K, L, M and O are known to appear, then it regenerates itself at the end of the photocycle. In the photocycl...Bacteriorhodopsin is the only protein in purple membrane. When illuminated, optically distinct intermediates K, L, M and O are known to appear, then it regenerates itself at the end of the photocycle. In the photocycle, the proton is released. So the function of purple membrane is a light-driven proton pump. The unique structure and function of purple membrane are an ideal material for investigating energy conversion. In recent years,展开更多
Bacteriorhodopsin in purple membrane was reconstituted into different lipid vesicles. The effect of three different lipids on the structure and function of bacteri-orhodopsin in lipid vesicles was studied by the obser...Bacteriorhodopsin in purple membrane was reconstituted into different lipid vesicles. The effect of three different lipids on the structure and function of bacteri-orhodopsin in lipid vesicles was studied by the observation on freeze-fracture eletron microscopy, the rotational diffusion of bacteriorhodopsin in lipid vesicles, the measurement of absorption spectrum, and the absorbance change with time. For these prepared samples, the results showed that DMPC was the stable lipid environment of bacteriorhodopsin; egg-pc causeed the loss of retinal chromophore of bacteriorhodopsin and it was not reversible change, cholesterol could stabilize the bacteriorhodopsin in lipid environment, but it caused the aggregation of bacteriorhodopsin.展开更多
Stoeckenius found the bacteriorhodopsin in purple membrane in the 1970s. Sinee then,much work has been done about the structure, function and application of molecular elec-tronics. In the late 1980s, the application o...Stoeckenius found the bacteriorhodopsin in purple membrane in the 1970s. Sinee then,much work has been done about the structure, function and application of molecular elec-tronics. In the late 1980s, the application of genetic engineering in molecular biology en-abled us to further understand the function of proton pump of purple membrane, but so far,展开更多
The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the...The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate.展开更多
The structural change of purple membrane during storage has been investigated by means of transmission electron microscope and atomic force microscope. It is found that many liposomes have spontaneously evolved from t...The structural change of purple membrane during storage has been investigated by means of transmission electron microscope and atomic force microscope. It is found that many liposomes have spontaneously evolved from the purple membrane sheets isolated three years ago. The membrane proteins on the liposomes, bacteriorhodopsin, are still presented as trimers in 2-D hexagonal structure, which is the same as that in natural cell membrane. However, the cytoplasmic surface of purple membrane faced outside on the liposomes.展开更多
Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were com- pared....Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were com- pared. Results show that 6 mmol/L CHAPS could induce some cracks and holes in PM. While Bateriorhodopsin (BR) was still presented as trimers in the two-dimensional hex- agonal structure. We assume that it was caused by the inter- action between CHAPS micellar and PM. Both absorption spectra and flash photolysis kinetic spectra indicated that BR抯 biochemical functions have been changed.展开更多
基金supported by the National Basic Research Program of China (2009CB930000, 2011CB606203)the National Natural Science Foundation of China (21034002)
文摘Bacteriorhodopsin is a well-known photoresponsive protein in the cell membrane of Halobacterium salinarum and exists in the patch called the purple membrane(PM).This letter reports,for the first time,its new function as a natural non-fouling substance for resisting cell adhesion.Mammalian cells such as murine preosteocytes MC3T3-E1 were seeded on the PM film.A significant resistance to cell adhesion on the film was found.
文摘Purple membrane, molecular weight 26ku, is synthesized by Holobacterium halobium on some parts of its cell membrane under a special condition combined by 248 residues,and 7 times acrossing membrane. Only one kind of protein, bacteriorhodopsin (BR), is on the membrane. After light excitation, BR goes through a series of intermediate processes K, L, M, N, O and recovers to BR. Purple membrane is an ideal biomaterial for photo energy transfer, and becomes a new focus field of many branches of science. Because the photocycle of BR is related to light-driven proton pump and photoelectric response,
文摘The purple membrane (PM) is found on the plasma membrane of Halobacteria Halobium,which contains an integral membrane protein, bacteriorhodopsin (BR). The structure andfunction of bacteriorhodopsin are rather clear at present. Protein to lipid ratio of the purplemembrane is 1:10 (mol/mol). Most of lipids of purple membranes are negatively charged atneutral pH.
基金Project supported by the National Natural Science Foundation of China
文摘Bacteriorhodopsin is the only protein in purple membrane. When illuminated, optically distinct intermediates K, L, M and O are known to appear, then it regenerates itself at the end of the photocycle. In the photocycle, the proton is released. So the function of purple membrane is a light-driven proton pump. The unique structure and function of purple membrane are an ideal material for investigating energy conversion. In recent years,
基金Thiswork was supported by the National Natural Science Foundation of China (Grant No. 30170235)the Chinese Academy of Sciences, and the Wellcome Trust of UK.
文摘Bacteriorhodopsin in purple membrane was reconstituted into different lipid vesicles. The effect of three different lipids on the structure and function of bacteri-orhodopsin in lipid vesicles was studied by the observation on freeze-fracture eletron microscopy, the rotational diffusion of bacteriorhodopsin in lipid vesicles, the measurement of absorption spectrum, and the absorbance change with time. For these prepared samples, the results showed that DMPC was the stable lipid environment of bacteriorhodopsin; egg-pc causeed the loss of retinal chromophore of bacteriorhodopsin and it was not reversible change, cholesterol could stabilize the bacteriorhodopsin in lipid environment, but it caused the aggregation of bacteriorhodopsin.
基金grant of Key Program of the Chinese Academy of Sciences.
文摘Stoeckenius found the bacteriorhodopsin in purple membrane in the 1970s. Sinee then,much work has been done about the structure, function and application of molecular elec-tronics. In the late 1980s, the application of genetic engineering in molecular biology en-abled us to further understand the function of proton pump of purple membrane, but so far,
文摘The steady absorption and kinetic changes of M 412 intermediate of the light and dark adapted bacteriorhodopsin (BR) solubilized by different concentrated Triton X 100 were investigated. The results indicated that the cooperative effect existing within the trimeric BR of native purple membrane (PM) was damaged in the system containing the surfactant since the component and structure of the bilayer lipid membrane in PM varied due to the solubilization of partial PM lipids by Triton X 100. The destruction to the cooperative effect of BR ultimately caused 13 cis retinal of the dark adapted BR to take part in BR photocycle and also to generate the deprotonated M 412 intermediate.
基金This work was supported by the State Key Basic I Research and Development Plan Project (Grant No. 1998010102).
文摘The structural change of purple membrane during storage has been investigated by means of transmission electron microscope and atomic force microscope. It is found that many liposomes have spontaneously evolved from the purple membrane sheets isolated three years ago. The membrane proteins on the liposomes, bacteriorhodopsin, are still presented as trimers in 2-D hexagonal structure, which is the same as that in natural cell membrane. However, the cytoplasmic surface of purple membrane faced outside on the liposomes.
基金supported by the State Key Basic Research and Development Plan Project(Grant No.199801012).
文摘Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were com- pared. Results show that 6 mmol/L CHAPS could induce some cracks and holes in PM. While Bateriorhodopsin (BR) was still presented as trimers in the two-dimensional hex- agonal structure. We assume that it was caused by the inter- action between CHAPS micellar and PM. Both absorption spectra and flash photolysis kinetic spectra indicated that BR抯 biochemical functions have been changed.