Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)...Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)C NMR,^(1)H-^(1)H COSY,HSQC,HMBC and ROESY),among which the stereostructure of compound 4 was determined by calcu-lated ECD.Furthermore,compounds 1-4 were evaluated for their aphidicidal activities.The insecticidal assay results showed that 1-4 exhibited moderate aphidicidal activities at the concentration of 0.1 mg/mL with the 24 h mortality rates ranging from 10.58 to 52.98%.Among them,pyrethrin D(2)showed the highest aphidicidal activity,with the 24 h mortality rate of 52.98%,which was slightly lower than the positive control(pyrethrin II,83.52%).展开更多
[Objective] The aim was to study characters of pollen grains of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium (Trev.) Vis.,morphological characters,fertility of pollen grain and germinatio...[Objective] The aim was to study characters of pollen grains of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium (Trev.) Vis.,morphological characters,fertility of pollen grain and germination percentage of seeds. [Method] Pollen grains were prepared by sulphuric acid-acetyl oxide decomposition method. The lengths of polar axis and equatorial axis of pollen grains were determined with general optical microscope. The morphology of pollen grains was observed with SEM (scanning electron microscope) and the typical visual fields of 2 500× (or 2 000×),7 000× were taken pictures. [Result] Comparing with the diploid control line,the pollen grains of five tetraploid lines which were tested were different from the diploid line in morphology,sculpture,etc.. 4 of the 5 tested samples were significant larger than the diploid line in size and one was similar to the diploid line. [Conclusion] This research provided references for breeding tetraploid improved varieties of Chrysanthemum cinerariifolium (Trev.) Vis. with good fertility and high germination percentage.展开更多
基金the Key Research and Development Program of Yunnan Province,China(202003AD 150006)the Cooperation Project with DR PLANT Company(2023).
文摘Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)C NMR,^(1)H-^(1)H COSY,HSQC,HMBC and ROESY),among which the stereostructure of compound 4 was determined by calcu-lated ECD.Furthermore,compounds 1-4 were evaluated for their aphidicidal activities.The insecticidal assay results showed that 1-4 exhibited moderate aphidicidal activities at the concentration of 0.1 mg/mL with the 24 h mortality rates ranging from 10.58 to 52.98%.Among them,pyrethrin D(2)showed the highest aphidicidal activity,with the 24 h mortality rate of 52.98%,which was slightly lower than the positive control(pyrethrin II,83.52%).
基金Supported by the Fundamental Research Funds for the Central Universities (SWJTU09BR221)~~
文摘[Objective] The aim was to study characters of pollen grains of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium (Trev.) Vis.,morphological characters,fertility of pollen grain and germination percentage of seeds. [Method] Pollen grains were prepared by sulphuric acid-acetyl oxide decomposition method. The lengths of polar axis and equatorial axis of pollen grains were determined with general optical microscope. The morphology of pollen grains was observed with SEM (scanning electron microscope) and the typical visual fields of 2 500× (or 2 000×),7 000× were taken pictures. [Result] Comparing with the diploid control line,the pollen grains of five tetraploid lines which were tested were different from the diploid line in morphology,sculpture,etc.. 4 of the 5 tested samples were significant larger than the diploid line in size and one was similar to the diploid line. [Conclusion] This research provided references for breeding tetraploid improved varieties of Chrysanthemum cinerariifolium (Trev.) Vis. with good fertility and high germination percentage.
