Dear editor,Though notorious for its tendency to induce recurrent neck abscess,pyriform sinus fistula is of importance despite its rarity.It usually presents diagnostic and therapeutic challenges.Because of post-infec...Dear editor,Though notorious for its tendency to induce recurrent neck abscess,pyriform sinus fistula is of importance despite its rarity.It usually presents diagnostic and therapeutic challenges.Because of post-infectious fi brosis,the embryologic origin of pyriform sinus fi stula is diffi cult to appreciate in certain cases.Here we present a case with empyema and mediastinal abscess caused by pyriform sinus fi stula and share our experience in the treatment of this patient.展开更多
Spider silk is capturing the attention of scientists for its mechanical properties,biocompatibility,and biodegradability.Spiders can produce six types of silks,as well as special glue,which are used for survival and r...Spider silk is capturing the attention of scientists for its mechanical properties,biocompatibility,and biodegradability.Spiders can produce six types of silks,as well as special glue,which are used for survival and reproduction.During the last years of research,scientists deciphered gene sequences and expressed the most common types of spider silks.However,matching the mechanical properties of recombinant spider silks to native ones is still a big challenge.Moreover,in-depth studies are mostly focused on natural and recombinant ampullate silks,and only a few studies showed achievements on pyriform spidroin(PySp).In this study,repeatable parts of PySp were expressed,purified,and spun into fibers.Recombinase cloning strategy allowed to create highly-repetitive region parts clones efficiently in comparison to the traditional restriction enzyme cloning technique.A cost-effective high-yield purification strategy was used.This study provides strategies that can help to design recombinant spider silks with the same mechanical properties as native spider silks.展开更多
Reconstruction of the hypopharynx and upper esophagus after resection of advanced pyriform sinus cancer. is usually complicated and time-consuming. Laryngotracheal flap was used in hypopharyngaesophageal reconstructio...Reconstruction of the hypopharynx and upper esophagus after resection of advanced pyriform sinus cancer. is usually complicated and time-consuming. Laryngotracheal flap was used in hypopharyngaesophageal reconstruction for 26 elderly patients with advanced pyriform sinus cancer Pharyngocutaneous fistula developed in 5 patients and healed spontaneously without further surgery. Full diet was resumed in all the patients. The surgical technique and its advantages and disadvantages are discussed. The laryngotracheal flap in the reconstruction of hypopharyngoesophageal defect for elderly patients is a procedure of choice.展开更多
目的:比较内镜CO_(2)激光烧灼术(ECLC)与颈部开放性切除术治疗先天性梨状窝瘘(CPSF)患儿的效果。方法:选取2021年3月至2022年3月该院收治的100例CPSF患儿进行前瞻性研究,按照随机数字表法将其分为对照组和观察组各50例。对照组行颈部开...目的:比较内镜CO_(2)激光烧灼术(ECLC)与颈部开放性切除术治疗先天性梨状窝瘘(CPSF)患儿的效果。方法:选取2021年3月至2022年3月该院收治的100例CPSF患儿进行前瞻性研究,按照随机数字表法将其分为对照组和观察组各50例。对照组行颈部开放性切除术治疗,观察组行ECLC治疗。比较两组手术相关指标(手术时间、术中出血量、平均住院时间、胃管留置时间)水平、术后1 d疼痛程度[视觉模拟评分法(VAS)]评分、术后3个月颈部美容评分和术后3、12个月治愈率,以及并发症发生率。结果:观察组手术时间、平均住院时间和胃管留置时间均短于对照组,术中出血量少于对照组,差异有统计学意义(P<0.05);观察组术后1 d VAS评分低于对照组,术后3个月颈部美容评分高于对照组,差异有统计学意义(P<0.05);观察组术后3个月治愈率为82.00%(41/50),低于对照组的100.00%(50/50),差异有统计学意义(P<0.05);两组术后12个月治愈率和并发症发生率比较,差异均无统计学意义(P>0.05)。结论:ECLC治疗CPSF患儿可降低手术相关指标水平和术后疼痛评分,提高颈部美容评分的效果优于颈部开放性切除术治疗,但术后3个月治愈率低于颈部开放性切除术,两种术式术后12个月的治愈率相当。展开更多
文摘Dear editor,Though notorious for its tendency to induce recurrent neck abscess,pyriform sinus fistula is of importance despite its rarity.It usually presents diagnostic and therapeutic challenges.Because of post-infectious fi brosis,the embryologic origin of pyriform sinus fi stula is diffi cult to appreciate in certain cases.Here we present a case with empyema and mediastinal abscess caused by pyriform sinus fi stula and share our experience in the treatment of this patient.
基金National Natural Science Foundation of China(No.31470836)Science and Technology Commission of Shanghai Municipality,China(No.19ZR1471000)。
文摘Spider silk is capturing the attention of scientists for its mechanical properties,biocompatibility,and biodegradability.Spiders can produce six types of silks,as well as special glue,which are used for survival and reproduction.During the last years of research,scientists deciphered gene sequences and expressed the most common types of spider silks.However,matching the mechanical properties of recombinant spider silks to native ones is still a big challenge.Moreover,in-depth studies are mostly focused on natural and recombinant ampullate silks,and only a few studies showed achievements on pyriform spidroin(PySp).In this study,repeatable parts of PySp were expressed,purified,and spun into fibers.Recombinase cloning strategy allowed to create highly-repetitive region parts clones efficiently in comparison to the traditional restriction enzyme cloning technique.A cost-effective high-yield purification strategy was used.This study provides strategies that can help to design recombinant spider silks with the same mechanical properties as native spider silks.
文摘Reconstruction of the hypopharynx and upper esophagus after resection of advanced pyriform sinus cancer. is usually complicated and time-consuming. Laryngotracheal flap was used in hypopharyngaesophageal reconstruction for 26 elderly patients with advanced pyriform sinus cancer Pharyngocutaneous fistula developed in 5 patients and healed spontaneously without further surgery. Full diet was resumed in all the patients. The surgical technique and its advantages and disadvantages are discussed. The laryngotracheal flap in the reconstruction of hypopharyngoesophageal defect for elderly patients is a procedure of choice.
文摘目的:比较内镜CO_(2)激光烧灼术(ECLC)与颈部开放性切除术治疗先天性梨状窝瘘(CPSF)患儿的效果。方法:选取2021年3月至2022年3月该院收治的100例CPSF患儿进行前瞻性研究,按照随机数字表法将其分为对照组和观察组各50例。对照组行颈部开放性切除术治疗,观察组行ECLC治疗。比较两组手术相关指标(手术时间、术中出血量、平均住院时间、胃管留置时间)水平、术后1 d疼痛程度[视觉模拟评分法(VAS)]评分、术后3个月颈部美容评分和术后3、12个月治愈率,以及并发症发生率。结果:观察组手术时间、平均住院时间和胃管留置时间均短于对照组,术中出血量少于对照组,差异有统计学意义(P<0.05);观察组术后1 d VAS评分低于对照组,术后3个月颈部美容评分高于对照组,差异有统计学意义(P<0.05);观察组术后3个月治愈率为82.00%(41/50),低于对照组的100.00%(50/50),差异有统计学意义(P<0.05);两组术后12个月治愈率和并发症发生率比较,差异均无统计学意义(P>0.05)。结论:ECLC治疗CPSF患儿可降低手术相关指标水平和术后疼痛评分,提高颈部美容评分的效果优于颈部开放性切除术治疗,但术后3个月治愈率低于颈部开放性切除术,两种术式术后12个月的治愈率相当。
