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A unique role of the pyrimidine de novo synthesis enzyme ODCase in Lysobacter enzymogenes
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作者 Mingming Yang Yunxiao Tan +13 位作者 Jiabing Ma Yingjia Zhao Xia Yan Nana Wang Pingping Wang Jiaqi Tan Suilong Ai Xiaofei Liang Bangshuai Chang Obadah E.A.Yousif Chao Zhao Bo Wang Guoliang Qian Lili Huang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第9期3066-3077,共12页
Bacterial species of the genus Lysobacter are environmentally ubiquitous with strong antifungal biocontrol potential.Heat-stable antifungal factor(HSAF)secreted by the biocontrol bacterium Lysobacter enzymogenes OH11 ... Bacterial species of the genus Lysobacter are environmentally ubiquitous with strong antifungal biocontrol potential.Heat-stable antifungal factor(HSAF)secreted by the biocontrol bacterium Lysobacter enzymogenes OH11 has broad-spectrum and highly efficient antifungal activity.Studying the biosynthetic regulations of HSAF would lay an important foundation for strain engineering toward improved HSAF production.In this work,we demonstrate that Le0752,an orotidine-5´-phosphate decarboxylase enzyme(ODCase)catalyzing a pivotal step of the UMP de novo biosynthesis pathway,is vital for HSAF-mediated antimicrobial activities and growth of L.enzymogenes OH11,but not for twitching motility.This gene regulates the production of HSAF by affecting the expression of lafB,a key gene in the HSAF biosynthesis operon,through the transcription factor Clp.Interestingly,bioinformatics analysis revealed that Le0752 belongs to the Group III ODCases,whereas its homologs in the closely related genera Xanthomonas and Stenotrophomonas belong to Group I,which contains most ODCases from Gram-positive bacteria,Gram-negative bacteria and cyanobacteria.Moreover,the Group I ODCase PXO_3614 from the Xanthomonas oryzae pv.oryzae PXO99A strain complemented the Le0752 mutant in regulating HSAF-mediated antagonistic activity.Together,these results highlight the important requirement of de novo pyrimidine biosynthetic enzymes for antibiotic HSAF production in L.enzymogenes,which lays an important foundation for improving HSAF production via metabolic flow design and for dissecting the regulatory functions of bacterial ODCases. 展开更多
关键词 ODCase pyrimidine de novo synthesis secondary metabolite HSAF Lysobacter enzymogenes
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Synthesis and antitumor activity of α-aminophosphonate derivatives containing thieno[2,3-d]pyrimidines 被引量:4
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作者 Yan-Chun Guo Jing Li +5 位作者 Jiao-Li Ma Zhi-Ran Yu Hai-Wei Wang Wen-Juan Zhu Xin-Cheng Liao Yu-Fen Zhao 《Chinese Chemical Letters》 SCIE CAS CSCD 2015年第6期755-758,共4页
Two series of thieno[2,3-d]pyrimidine derivatives were designed and synthesized, in which bioactive α-aminophosphonate subunits were introduced at the N3 position through an N-N bond connection. The in vitro cytotoxi... Two series of thieno[2,3-d]pyrimidine derivatives were designed and synthesized, in which bioactive α-aminophosphonate subunits were introduced at the N3 position through an N-N bond connection. The in vitro cytotoxic activity of the novel compounds was tested against human esophageal carcinoma cells (EC109), human hepatocarcinoma cells (HepG2), human gastric carcinoma cells (MGC-803), respectively, by the MTT method. The evaluation results revealed that compounds fimb, 6mf, 6mg, 6rid and 6nh exerted the most potent inhibition against HepG2, MGC-803 and EC109 cells, respectively. In particular, compound 6rag presented excellent inhibitory effect against HepG2 (91.2%) and MGC-803 (94.4%) cells. 展开更多
关键词 α-Aminophosphonate derivatives Thieno[2 3-d]pyrimidine synthesis Antitumor
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