Peutz-Jeghers syndrome(PJS)is a polygenic autosomal dominant disease characterized by multiple gastrointestinal polyps and pigmentation of the mucosa and skin.While there are a few reports regarding successful treatme...Peutz-Jeghers syndrome(PJS)is a polygenic autosomal dominant disease characterized by multiple gastrointestinal polyps and pigmentation of the mucosa and skin.While there are a few reports regarding successful treatment of intestinal polyps in PJS,there is little research regarding treatment of mucocutaneous melanosis.This study investigated the many advantages of using a Q-switched alexandrite laser to treat mucocutaneous melanosis.In this case,a 19-year-old male with PJS presented with labial lentigines and received two Q-switched alexandrite laser treatments in 2018.Subsequently,the efficacy of the treatment was evaluated.The result of the evaluation was that,after the two laser treatments,the labial lentigines were successfully removed,and there were no complications.展开更多
In order to determine the appropriate treatment interval, 267 patients who underwent 3 sessions of treatment with Q-switched alexandrite laser were divided into 4 groups according to treatment interval, and their clin...In order to determine the appropriate treatment interval, 267 patients who underwent 3 sessions of treatment with Q-switched alexandrite laser were divided into 4 groups according to treatment interval, and their clinical responses were compared. Among them, 187 were asked about the process of pigment fading. Moreover, light and transmission electron microscopy were performed. It was noted that the clinical response of the 5 - 6 month interval group was significantly better than that of the 3 - 4 month group, but showed no significant difference from that of the 7 - 8 or ≥ 9 month group. 80.21% of investigated patients stated that marked pigment fading could no longer be observed 7 months after irradiation. 4 months after irradiation, the degenerated melanosomes and cell debris were still scattered among collagen fibers, scavenged gradually by macrophage. In conclusion, an appropriate treatment interval is 5 - 6 months.展开更多
Background Trichophyton rubrum （T. rubrum） is the most common causative agent of dermatophytosis worldwide. In this study, we examined the effect of laser irradiation on the growth and morphology of T. rubrum. Metho...Background Trichophyton rubrum （T. rubrum） is the most common causative agent of dermatophytosis worldwide. In this study, we examined the effect of laser irradiation on the growth and morphology of T. rubrum. Methods Colonies of T. rubrum were isolated （one colony per plate）, and randomly assigned to 5 treatment groups： Q-switched 694 nm ruby laser treatment, long-pulsed Nd：YAG 1064 nm laser treatment, intense pulsed light （IPL） treatment, 308 nm excimer laser treatment and the blank control group without treatment. Standardized photographs were obtained from grown-up fungal plates prior to treatment. Colonies were then exposed to various wavelengths and fluences of laser light. To compare the growth of colonies, they were re-photographed under identical conditions three and six days post-treatment. To investigate the morphology of T. rubrum, scanning electron microscope （SEM） and transmission electron microscope （TEM） images were obtained from specimens exposed to 24 hours of laser treatment. Results Growth of T. rubrum colonies was significantly inhibited following irradiation by 694 nm Q-switched and 1064 nm long-pulsed Nd：YAG lasers. Other treatments exerted little or no effect. Q-switched laser irradiation exerted a stronger growth inhibitory effect than long-pulsed Nd：YAG laser irradiation. Following treatment by the Q-switched ruby laser system, T. rubrum hyphae became shrunken and deflated, and SEM images revealed rough, fractured hyphal surfaces, punctured with small destructive holes. TEM images showed that the hyphae were degenerating, as evidenced by the irregular shape of hyphae, rough and loose cell wall, and obscure cytoplasmic texture. Initially high electron density structure was visible in the cell; later, low-density structure appeared as a result of cytoplasmic dissolution. In contrast, the blank control group showed no obvious changes in morphology.展开更多
文摘Peutz-Jeghers syndrome(PJS)is a polygenic autosomal dominant disease characterized by multiple gastrointestinal polyps and pigmentation of the mucosa and skin.While there are a few reports regarding successful treatment of intestinal polyps in PJS,there is little research regarding treatment of mucocutaneous melanosis.This study investigated the many advantages of using a Q-switched alexandrite laser to treat mucocutaneous melanosis.In this case,a 19-year-old male with PJS presented with labial lentigines and received two Q-switched alexandrite laser treatments in 2018.Subsequently,the efficacy of the treatment was evaluated.The result of the evaluation was that,after the two laser treatments,the labial lentigines were successfully removed,and there were no complications.
基金This work was supported by the Shanghai Municipal Natural Science under the Grant (97-181).
文摘In order to determine the appropriate treatment interval, 267 patients who underwent 3 sessions of treatment with Q-switched alexandrite laser were divided into 4 groups according to treatment interval, and their clinical responses were compared. Among them, 187 were asked about the process of pigment fading. Moreover, light and transmission electron microscopy were performed. It was noted that the clinical response of the 5 - 6 month interval group was significantly better than that of the 3 - 4 month group, but showed no significant difference from that of the 7 - 8 or ≥ 9 month group. 80.21% of investigated patients stated that marked pigment fading could no longer be observed 7 months after irradiation. 4 months after irradiation, the degenerated melanosomes and cell debris were still scattered among collagen fibers, scavenged gradually by macrophage. In conclusion, an appropriate treatment interval is 5 - 6 months.
文摘Background Trichophyton rubrum （T. rubrum） is the most common causative agent of dermatophytosis worldwide. In this study, we examined the effect of laser irradiation on the growth and morphology of T. rubrum. Methods Colonies of T. rubrum were isolated （one colony per plate）, and randomly assigned to 5 treatment groups： Q-switched 694 nm ruby laser treatment, long-pulsed Nd：YAG 1064 nm laser treatment, intense pulsed light （IPL） treatment, 308 nm excimer laser treatment and the blank control group without treatment. Standardized photographs were obtained from grown-up fungal plates prior to treatment. Colonies were then exposed to various wavelengths and fluences of laser light. To compare the growth of colonies, they were re-photographed under identical conditions three and six days post-treatment. To investigate the morphology of T. rubrum, scanning electron microscope （SEM） and transmission electron microscope （TEM） images were obtained from specimens exposed to 24 hours of laser treatment. Results Growth of T. rubrum colonies was significantly inhibited following irradiation by 694 nm Q-switched and 1064 nm long-pulsed Nd：YAG lasers. Other treatments exerted little or no effect. Q-switched laser irradiation exerted a stronger growth inhibitory effect than long-pulsed Nd：YAG laser irradiation. Following treatment by the Q-switched ruby laser system, T. rubrum hyphae became shrunken and deflated, and SEM images revealed rough, fractured hyphal surfaces, punctured with small destructive holes. TEM images showed that the hyphae were degenerating, as evidenced by the irregular shape of hyphae, rough and loose cell wall, and obscure cytoplasmic texture. Initially high electron density structure was visible in the cell; later, low-density structure appeared as a result of cytoplasmic dissolution. In contrast, the blank control group showed no obvious changes in morphology.
