An understanding of soil microbial communities is crucial in roadside soil environmental assessments.The 16S rRNA se quencing of a stressed microbial community in soil adjacent to the Qinghai-Tibet Highway(QTH)reveale...An understanding of soil microbial communities is crucial in roadside soil environmental assessments.The 16S rRNA se quencing of a stressed microbial community in soil adjacent to the Qinghai-Tibet Highway(QTH)revealed that the accu mulation of heavy metals(over about 10 years)has affected the diversity of bacterial abundance and microbial community structure.The proximity of a sampling site to the QTH/Qinghai-Tibet Railway(QTR),which is effectively a measure of the density of human engineering,was the dominant factor influencing bacterial community diversity.The diversity of bacterial communities shows that 16S rRNA gene abundance decreased in relation to proximity to the QTH and QTR in both alpine wetland and meadow areas.The dominant phyla across all samples were Actinobacteria and Proteobacteria.The concentration of Cr and Cd in the soil were positively correlated with proximity to the QTH and QTR(MC/WC sam pling sites),and Ni,Co,and V were positively correlated with proximity to the QTH and QTR(MA/WA sampling sites).The results presented in this study provide an insight into the relationships among heavy metals and soil microbial commu nities,and have important implications for assessing and predicting the impacts of human-induced activities from the QTH and QTR in such an extreme and fragile environment.展开更多
The Qinghai-Tibet Railway(QTR) passes through 281 km of sandy land, 11.07 km of which causes serious sand damage to the railway and thus, the control of blown sand is important for the safe operation of the railway. C...The Qinghai-Tibet Railway(QTR) passes through 281 km of sandy land, 11.07 km of which causes serious sand damage to the railway and thus, the control of blown sand is important for the safe operation of the railway. Construction of the railway and sand prevention system greatly changed the blown sand transport of the primary surface. Effective and feasible sand-control measures include stone checkerboard barriers(SCBs), sand fences(SFs), and gravel coverings. This study simulated the embankments, SCBs and SFs of the QTR in a wind tunnel, and analyzed their respective wind profile, sand deposition, and sand-blocking rate(SBR) in conjunction with field data, aiming at studying the influence of Golmud-Lhasa section of the QTR and sand prevention system on blown sand transport. The results of wind tunnel experiments showed that wind speed increased by 67.7%–77.3% at the upwind shoulder of the embankment and decreased by 50.0%–83.3% at upwind foot of embankment. Wind speed decreased by 50.0%–100.0% after passing through the first SF, and 72.2%–100.0% after the first row of stones within the first SCB grid. In the experiment of sand deposition, the higher the wind speed, the lower the SBR of SCB and SF. From field investigation, the amount of sand blocked by the four SFs decreased exponentially and its SBR was about 50.0%. By contrast, SCB could only block lower amounts of sand, but had a higher SBR(96.7%) than SF. Although, results show that SFs and SCBs along the Golmud-Lhasa section of the QTR provide an obvious sand blocking effect, they lead to the deposition of a large amount of sand, which forms artificial dunes and becomes a new source of sand damage.展开更多
基金funded by a grant from the Cold and Arid Regions Environmental and the Engineering Research Institute of the Chinese Academy of Sciences Nos. HHS-TSS-STS-1505 and 55Y855Z11, CAS "Light of West China" Program, Frontier Science Research Program of Chineses Academy of Scienc No. QYZDJ- SSW_SMC011
文摘An understanding of soil microbial communities is crucial in roadside soil environmental assessments.The 16S rRNA se quencing of a stressed microbial community in soil adjacent to the Qinghai-Tibet Highway(QTH)revealed that the accu mulation of heavy metals(over about 10 years)has affected the diversity of bacterial abundance and microbial community structure.The proximity of a sampling site to the QTH/Qinghai-Tibet Railway(QTR),which is effectively a measure of the density of human engineering,was the dominant factor influencing bacterial community diversity.The diversity of bacterial communities shows that 16S rRNA gene abundance decreased in relation to proximity to the QTH and QTR in both alpine wetland and meadow areas.The dominant phyla across all samples were Actinobacteria and Proteobacteria.The concentration of Cr and Cd in the soil were positively correlated with proximity to the QTH and QTR(MC/WC sam pling sites),and Ni,Co,and V were positively correlated with proximity to the QTH and QTR(MA/WA sampling sites).The results presented in this study provide an insight into the relationships among heavy metals and soil microbial commu nities,and have important implications for assessing and predicting the impacts of human-induced activities from the QTH and QTR in such an extreme and fragile environment.
基金Under the auspices of National Natural Science Foundation of China(No.40930741)National Basic Research Program of China(No.2012CB026105)
文摘The Qinghai-Tibet Railway(QTR) passes through 281 km of sandy land, 11.07 km of which causes serious sand damage to the railway and thus, the control of blown sand is important for the safe operation of the railway. Construction of the railway and sand prevention system greatly changed the blown sand transport of the primary surface. Effective and feasible sand-control measures include stone checkerboard barriers(SCBs), sand fences(SFs), and gravel coverings. This study simulated the embankments, SCBs and SFs of the QTR in a wind tunnel, and analyzed their respective wind profile, sand deposition, and sand-blocking rate(SBR) in conjunction with field data, aiming at studying the influence of Golmud-Lhasa section of the QTR and sand prevention system on blown sand transport. The results of wind tunnel experiments showed that wind speed increased by 67.7%–77.3% at the upwind shoulder of the embankment and decreased by 50.0%–83.3% at upwind foot of embankment. Wind speed decreased by 50.0%–100.0% after passing through the first SF, and 72.2%–100.0% after the first row of stones within the first SCB grid. In the experiment of sand deposition, the higher the wind speed, the lower the SBR of SCB and SF. From field investigation, the amount of sand blocked by the four SFs decreased exponentially and its SBR was about 50.0%. By contrast, SCB could only block lower amounts of sand, but had a higher SBR(96.7%) than SF. Although, results show that SFs and SCBs along the Golmud-Lhasa section of the QTR provide an obvious sand blocking effect, they lead to the deposition of a large amount of sand, which forms artificial dunes and becomes a new source of sand damage.