As one of the most distinguished features of Chinese Culture,collective oriented culture is the deep structure of Chinese cultural psychology which has shaped Chinese character and influenced their behavior.As a resul...As one of the most distinguished features of Chinese Culture,collective oriented culture is the deep structure of Chinese cultural psychology which has shaped Chinese character and influenced their behavior.As a result of it,Chinese students suffer this negative effect from collective orientated culture when acquiring English,especially in oral English acquisition.TEFL teachers should be aware of cultural psychology and try their best to minimize its negative effect instead of neglecting or even enlarging it during oral English teaching.展开更多
Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activit...Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.展开更多
Culture collection of the Technical University of Budapest (TUB) comprises app ro x. 2800 microorganisms with special emphasis on lignocellulose degrading fungi a nd actinomycetes. For isolation and screening programs...Culture collection of the Technical University of Budapest (TUB) comprises app ro x. 2800 microorganisms with special emphasis on lignocellulose degrading fungi a nd actinomycetes. For isolation and screening programs, 460 soils are stored at +5 ℃. They were collected in 58 countries, including extreme environments. There are 860 Trichoderma strains in TUB collection, representing 45 count ries and 173 soils and/or tree barks as isolation sources. Trichoderma spp . selected from soils in the tundra region (Rocky Mountains, Colorado) grow at l ow temperatures such as 5-10 ℃. These strains practically do not grow at 30 ℃ . On the contrary, many Trichoderma strains were isolated from tropical or s ubtropical soils and decaying plant materials at 35-40 ℃ in Brazil, Peru, Cost a Rica, Guatemala, Hawaii, Tanzania, Thailand and Indonesia. Trichoderma str ains tolerating limited oxygen supply were selected from river muds and sediment s. Trichoderma spp. tolerating or partially degrading 2,4-dichlorophenol a nd 2,4,6-trichlorophenol in 20 mg/kg concentration were selected from polluted soils. As a part of an attempt to assess the global biodiversity of Trichoderma, researchers of Technical University of Vienna and Agriculture Canada, in collab oration with TUB collection, identified and characterized seven new assumed taxa of Trichoderma from Eurasia.展开更多
Objective:To establish a new way to collect superovulated oocytes or zygotes repeatedlyfrom an individual mouse.Methods:Superovulations were induced by injection PMSG and hCG in Kunming strainmice.The ampullaes of ovi...Objective:To establish a new way to collect superovulated oocytes or zygotes repeatedlyfrom an individual mouse.Methods:Superovulations were induced by injection PMSG and hCG in Kunming strainmice.The ampullaes of oviduct of all anaesthetised mouse were put in a specially designed'U'sink and released.The second and third times of PMSG injection were made on the sixth day andeleventh day after the first superovulation injection.The capacity of development was examinedby in vitro culture of parthenogenesis activation oocytes.Results:Development to blastocyst stage was not significantly different between the first andsecond time collection.The percentage of blastocyst stage in CD and Sr^(++) treatment was signifi-cantly higher(P<0.05)than the oocytes treated in CB and Sr^(++).Conclusion:This method enables us to collect oocytes or zygotes repeatedly from one individ-ual mouse in an interval as short as 5 days and without influence on the quality of oocytes.展开更多
This paper analyzes the impact of past trauma on national behavior in times of threat, applying Volkan＇s （2001, 2004） theory of multigenerational impact of trauma on large groups, and La Capra＇s （2014） theory of...This paper analyzes the impact of past trauma on national behavior in times of threat, applying Volkan＇s （2001, 2004） theory of multigenerational impact of trauma on large groups, and La Capra＇s （2014） theory of the use of the concept of individuals＇ trauma for understanding the behavior of traumatized collectives. The study of the Israeli response to the abduction and murder of three teenagers in summer 2014 is consistent with these theories, and shows how a collective with trauma in its past behaves similarly to a posttraumatic person. The paper contends that due to its traumatic past, the Jewish Israeli public perceived a tragic but limited （from a national security standpoint） hostile action as a grave collective threat, which invoked past demons and brought back existential fears. These feelings were inflamed by the Israeli media and leaders, paving the way for an escalation of the event into an armed battle.展开更多
The prime function of the Philippine National Collection of Microorganisms (PNCM), being the national repository of microbial strains, is to collect and preserve strains for their continued viability and availability ...The prime function of the Philippine National Collection of Microorganisms (PNCM), being the national repository of microbial strains, is to collect and preserve strains for their continued viability and availability for future use. To date, a total of 2144 strains of bacteria (1357), yeasts (250), filamentous fungi (377), algae (14), and strains still to be identified (146) are maintained at the PNCM. These are preserved and maintained using various methods which include modified liquid drying (lyophilization), ultra-low temperature (-70℃) storage in 10% glycerol, storage in sterile soil, distilled water and overlaying with mineral oil. Periodic viability testing is done to assess the stability of these preserved cultures under storage. Aside from preservation and maintenance of cultures, the PNCM is also involved in several research activities. One of these is the isolation, characterization and identification of some Vibrio isolates from the Philippines. Details on this particular study is presented in this report.展开更多
When the umbrellas were held up against the tear gas attack in the protest on September 28, 2014, the hot issue raised by the marathon-like Yellow Umbrella Movement was not only the matter of politics but also a coinc...When the umbrellas were held up against the tear gas attack in the protest on September 28, 2014, the hot issue raised by the marathon-like Yellow Umbrella Movement was not only the matter of politics but also a coincidence created for the Hong Kong locals to face, or to be faced with an already moot cliche： How the Hongkongese can redef＇me and redeem their almost lost socio-cultural identity, particularly under the increasingly hegemonic influences and the socio-cultural invasion of the mainland after the 1997 Handover. My present paper is not intended to discuss the topic of identity on the platform of politics, or the postcolonial studies such as transculturation or cultural hybridty. But instead, I am far more interested in locating such issue on the aesthetic dimension of collective memory, which is revealed in two local contemporary Hong Kong compositions. In the process of shaping and reshaping a form of＂HongKongeseness＂ in which the composers tend to create and the local listeners tend to experience, albeit transient, can appear in every nuance of the sonic metaphor embedded in the pre-existing indigenous tunes of a self-contained compositional work.展开更多
As a traditional local opera,Huagu Opera in southern Anhui have faced a threat of dying out as a result of the change of social and ecological environment in the rural areas of China as well as the impact of multiple ...As a traditional local opera,Huagu Opera in southern Anhui have faced a threat of dying out as a result of the change of social and ecological environment in the rural areas of China as well as the impact of multiple entertainment since the reform and openingup.Based on the analysis of its current situation,this paper proposes ways to preserve and inherit Huagu Opera by combining traditional means with digital collection technology.展开更多
文摘As one of the most distinguished features of Chinese Culture,collective oriented culture is the deep structure of Chinese cultural psychology which has shaped Chinese character and influenced their behavior.As a result of it,Chinese students suffer this negative effect from collective orientated culture when acquiring English,especially in oral English acquisition.TEFL teachers should be aware of cultural psychology and try their best to minimize its negative effect instead of neglecting or even enlarging it during oral English teaching.
文摘Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.
文摘Culture collection of the Technical University of Budapest (TUB) comprises app ro x. 2800 microorganisms with special emphasis on lignocellulose degrading fungi a nd actinomycetes. For isolation and screening programs, 460 soils are stored at +5 ℃. They were collected in 58 countries, including extreme environments. There are 860 Trichoderma strains in TUB collection, representing 45 count ries and 173 soils and/or tree barks as isolation sources. Trichoderma spp . selected from soils in the tundra region (Rocky Mountains, Colorado) grow at l ow temperatures such as 5-10 ℃. These strains practically do not grow at 30 ℃ . On the contrary, many Trichoderma strains were isolated from tropical or s ubtropical soils and decaying plant materials at 35-40 ℃ in Brazil, Peru, Cost a Rica, Guatemala, Hawaii, Tanzania, Thailand and Indonesia. Trichoderma str ains tolerating limited oxygen supply were selected from river muds and sediment s. Trichoderma spp. tolerating or partially degrading 2,4-dichlorophenol a nd 2,4,6-trichlorophenol in 20 mg/kg concentration were selected from polluted soils. As a part of an attempt to assess the global biodiversity of Trichoderma, researchers of Technical University of Vienna and Agriculture Canada, in collab oration with TUB collection, identified and characterized seven new assumed taxa of Trichoderma from Eurasia.
文摘Objective:To establish a new way to collect superovulated oocytes or zygotes repeatedlyfrom an individual mouse.Methods:Superovulations were induced by injection PMSG and hCG in Kunming strainmice.The ampullaes of oviduct of all anaesthetised mouse were put in a specially designed'U'sink and released.The second and third times of PMSG injection were made on the sixth day andeleventh day after the first superovulation injection.The capacity of development was examinedby in vitro culture of parthenogenesis activation oocytes.Results:Development to blastocyst stage was not significantly different between the first andsecond time collection.The percentage of blastocyst stage in CD and Sr^(++) treatment was signifi-cantly higher(P<0.05)than the oocytes treated in CB and Sr^(++).Conclusion:This method enables us to collect oocytes or zygotes repeatedly from one individ-ual mouse in an interval as short as 5 days and without influence on the quality of oocytes.
文摘This paper analyzes the impact of past trauma on national behavior in times of threat, applying Volkan＇s （2001, 2004） theory of multigenerational impact of trauma on large groups, and La Capra＇s （2014） theory of the use of the concept of individuals＇ trauma for understanding the behavior of traumatized collectives. The study of the Israeli response to the abduction and murder of three teenagers in summer 2014 is consistent with these theories, and shows how a collective with trauma in its past behaves similarly to a posttraumatic person. The paper contends that due to its traumatic past, the Jewish Israeli public perceived a tragic but limited （from a national security standpoint） hostile action as a grave collective threat, which invoked past demons and brought back existential fears. These feelings were inflamed by the Israeli media and leaders, paving the way for an escalation of the event into an armed battle.
文摘The prime function of the Philippine National Collection of Microorganisms (PNCM), being the national repository of microbial strains, is to collect and preserve strains for their continued viability and availability for future use. To date, a total of 2144 strains of bacteria (1357), yeasts (250), filamentous fungi (377), algae (14), and strains still to be identified (146) are maintained at the PNCM. These are preserved and maintained using various methods which include modified liquid drying (lyophilization), ultra-low temperature (-70℃) storage in 10% glycerol, storage in sterile soil, distilled water and overlaying with mineral oil. Periodic viability testing is done to assess the stability of these preserved cultures under storage. Aside from preservation and maintenance of cultures, the PNCM is also involved in several research activities. One of these is the isolation, characterization and identification of some Vibrio isolates from the Philippines. Details on this particular study is presented in this report.
文摘When the umbrellas were held up against the tear gas attack in the protest on September 28, 2014, the hot issue raised by the marathon-like Yellow Umbrella Movement was not only the matter of politics but also a coincidence created for the Hong Kong locals to face, or to be faced with an already moot cliche： How the Hongkongese can redef＇me and redeem their almost lost socio-cultural identity, particularly under the increasingly hegemonic influences and the socio-cultural invasion of the mainland after the 1997 Handover. My present paper is not intended to discuss the topic of identity on the platform of politics, or the postcolonial studies such as transculturation or cultural hybridty. But instead, I am far more interested in locating such issue on the aesthetic dimension of collective memory, which is revealed in two local contemporary Hong Kong compositions. In the process of shaping and reshaping a form of＂HongKongeseness＂ in which the composers tend to create and the local listeners tend to experience, albeit transient, can appear in every nuance of the sonic metaphor embedded in the pre-existing indigenous tunes of a self-contained compositional work.
基金Anhui Special Funds for Domestic Visiting Scholars(gxfxZD2016245)Anhui Social Science Key Research Project(SK2016A0933)
文摘As a traditional local opera,Huagu Opera in southern Anhui have faced a threat of dying out as a result of the change of social and ecological environment in the rural areas of China as well as the impact of multiple entertainment since the reform and openingup.Based on the analysis of its current situation,this paper proposes ways to preserve and inherit Huagu Opera by combining traditional means with digital collection technology.
