The world's only captive Yangtze River dolphin, QiQi,died on July 14 at the Wuhan-based Institute of Hydrobiology (IHB) of the Chinese Academy of Sciences (CAS). At age of about 25, well advanced in years for a fr...The world's only captive Yangtze River dolphin, QiQi,died on July 14 at the Wuhan-based Institute of Hydrobiology (IHB) of the Chinese Academy of Sciences (CAS). At age of about 25, well advanced in years for a freshwater dolphin, his death is considered as a natural one.展开更多
目的探讨大七气汤加减方(modified da qiqi decoction,MDQD)对子宫内膜异位症大鼠P450、COX-2表达的影响。方法采用SD雌性大鼠子宫内膜自体移植法建模,3周后按随机数字表法分为大七气汤加减方高剂量组(n=9)与低剂量组(n=9)、孕三烯酮组(...目的探讨大七气汤加减方(modified da qiqi decoction,MDQD)对子宫内膜异位症大鼠P450、COX-2表达的影响。方法采用SD雌性大鼠子宫内膜自体移植法建模,3周后按随机数字表法分为大七气汤加减方高剂量组(n=9)与低剂量组(n=9)、孕三烯酮组(n=9)、模型组(n=10)及假手术组(n=10)。给药3周后,用发光免疫分析法和ELISA法检测大鼠血清与异位内膜组织雌二醇(E2)与前列腺素E2(PGE2),用免疫组化法和RT-PCR技术检测异位组织芳香化酶P450(P450arom)、环氧化酶-2(COX-2)蛋白与mRNA的表达。结果 MDQD高剂量组大鼠血清E2、PGE2,组织E2、PGE2,P450与COX-2蛋白及mRNA明显低于模型组(P<0.01),与孕三烯酮组比较无明显差异(P>0.05)。结论大七气汤加减方能降低内异症大鼠P450、COX-2和PGE2的含量,通过抑制雌激素生成代谢降低外周血与局部病灶雌激素水平,抑制异位内膜生长。展开更多
文摘The world's only captive Yangtze River dolphin, QiQi,died on July 14 at the Wuhan-based Institute of Hydrobiology (IHB) of the Chinese Academy of Sciences (CAS). At age of about 25, well advanced in years for a freshwater dolphin, his death is considered as a natural one.
文摘目的探讨大七气汤加减方(modified da qiqi decoction,MDQD)对子宫内膜异位症大鼠P450、COX-2表达的影响。方法采用SD雌性大鼠子宫内膜自体移植法建模,3周后按随机数字表法分为大七气汤加减方高剂量组(n=9)与低剂量组(n=9)、孕三烯酮组(n=9)、模型组(n=10)及假手术组(n=10)。给药3周后,用发光免疫分析法和ELISA法检测大鼠血清与异位内膜组织雌二醇(E2)与前列腺素E2(PGE2),用免疫组化法和RT-PCR技术检测异位组织芳香化酶P450(P450arom)、环氧化酶-2(COX-2)蛋白与mRNA的表达。结果 MDQD高剂量组大鼠血清E2、PGE2,组织E2、PGE2,P450与COX-2蛋白及mRNA明显低于模型组(P<0.01),与孕三烯酮组比较无明显差异(P>0.05)。结论大七气汤加减方能降低内异症大鼠P450、COX-2和PGE2的含量,通过抑制雌激素生成代谢降低外周血与局部病灶雌激素水平,抑制异位内膜生长。