《针灸大成》关于气海穴临床应用的文献研究

目的:通过检索《针灸大成》中与气海穴治疗作用相关的文献条文,总结气海穴在治疗各系统疾病中运用频次较高的疾病及其配穴规律,为临床针灸对气海穴的使用提供理论支持。方法:以《中华医典》(第五版)中《针灸大成》作为文献检索来源,将气海穴及气海穴的别称“脖胦”“下肓”“丹田”“肓之原”“肓原”“下言”和“气泽”为检索词,用计算机检索工具及人工检索相结合的方法检索符合要求的文献条文,通过建立本研究的数据库,频次分析、条形统计图比较分析等方法,总结出气海穴在治疗各系统疾病中的运用频次及其配穴规律。结果:在《针灸大成》所涉及的条文中,气海穴尤善治疗内科疾病,在治疗内科疾病中排名前3位的是脾胃系病症、气血津液疾病、肾系病症和妇科疾病,气海穴配穴习惯为上下配穴法,同名经配穴法,以及前后配穴法,其中主要为前后配穴法和同名经配穴法。结论:气海穴《针灸大成》中单穴应用占比最高,而在气海穴众多配穴中,运用了本经配穴法、上下配穴法、前后配穴法,配穴归经主要来自任脉和足太阳膀胱经。同名经配穴法,同气相求,可增加疗效;与气海穴配伍较多的足太阳膀胱经以背腧穴为主,此为前后配穴法,亦称腹背阴阳配穴法,腹部为阴,腰背为阳,前后配穴法可起到“从阳引阴”亦可“从阴引阳”的作用,以达到调节阴阳,调和脏法,调畅经络的目的。

热敏灸对阴虚质、阳虚质两种体质人群正常高值血压的影响

目的观察热敏灸治疗对阴虚质、阳虚质两种体质人群正常高值血压的影响。方法将86例正常高值血压人群随机分为治疗组42例(阴虚质20例,阳虚质22例)和对照组44例(阴虚质19例,阳虚质25例)。对照组采用健康教育、饮食指导等一般措施,治疗组在对照组基础上采用热敏灸治疗。两组均每星期随访2次并测量血压。治疗1个月后,观察并比较两组不同体质人群的临床疗效、高血压确诊率及转平和质百分率。结果治疗组阴虚质和阳虚质人群总有效率分别为90.0%和100.0%,对照组分别为78.9%和92.0%,两组比较差异均具有统计学意义(P<0.05)。治疗组阴虚质和阳虚质人群治疗后高血压确诊率分别为15.0%和9.1%,对照组分别为36.8%和16.0%,两组比较差异均具有统计学意义(P<0.05)。治疗组治疗后阴虚质或阳虚质人群转平和质百分率为23.8%,对照组为13.6%,两组比较差异具有统计学意义(P<0.05)。结论热敏灸治疗能降低阴虚质、阳虚质两种体质人群正常高值血压及高血压发病率,并可有效纠正偏颇体质。

李志道教授“补三气”为主临床刺法举隅

李志道教授依据中医基础理论辨证取穴,总结出以任脉三穴为主的"补三气"疗法。"补三气"法即以任脉之膻中、中脘、气海三穴为主穴组成基础方,依次补充清气、谷气及元气。临床中,根据三气虚损程度的不同而有所侧重,随证灵活配穴,分别治疗以心肺气虚、脾胃气虚和先天元气不足的不同临床表现为主的疾病,并举例说明之。

阴虚体质任脉线上体表温度、循经红外辐射轨迹特征及电针对其的影响

目的:观察自然状态下平和、阴虚体质任脉线上体表温度、循经红外辐射轨迹特征(IRRTM),并探讨电针对其的影响。方法:应用红外热像仪观察17名阴虚体质受试者电针前后任脉体表温度、IRRTM并与平和体质进行比较。结果:在自然状态下:阴虚体质任脉上平均体表温度,膻中穴、下脘穴、气海穴体表均高于平和体质,其中膻中穴具有显著性差异(P<0.05);平和质3个穴位进行比较,膻中穴体表温度显著高于下脘穴与气海穴(P<0.01);阴虚体质3个穴位进行比较,膻中穴体表温度显著高于气海穴(P<0.05);平和体质组IRRTM显示的连续性更好、范围更长;阴虚体质组IRRTM显示范围较宽,与周边组织分界不如平和体质组清晰。电针中脘穴20min:与本组自然状态同穴位比较,平和体质下脘穴体表温度显著升高(P<0.05);阴虚体质下脘穴体表温度显著降低(P<0.05)。电针可诱发平和体质任脉IRRTM轨迹变长,而对已能显示任脉IRRTM轨迹的受试者,电针则可使其变得连续、规整;电针可使部分阴虚体质受试者任脉IRRTM轨迹出现与任脉循经红外辐射轨迹走向相同的低温线,分布在膻中穴周围。结论:阴虚体质任脉上体表温度高,在膻中穴上明显;电针中脘穴能升高平和体质下脘穴体表温度,却降低阴虚体质下脘穴体表温度;表明针刺对不同体质受试者IRRTM能产生不同的影响,IRRTM与人体的能量代谢有密切的关系。

Study on the regulatory effect of herbal cake-partitioned moxibustion on colonic CD206,AMPK and TSC2 in rats with Crohn disease

Objective:To explore the mechanism of herbal cake-partitioned moxibustion in Crohn disease(CD)treatment by observing the effect of herbal cake-partitioned moxibustion on protein expressions of colonic M2 macrophage marker CD206,AMP-activated protein kinase(AMPK)and tuberous sclerosis complex(TSC)2.Methods:Twenty-six specific pathogen free male rats were randomly divided into a normal group,a model group and a herbal cake-partitioned moxibustion group.The CD model was prepared by enema with the mixture of 5%(W/V)2,4,6-trinitrobenzene sulfonic acid(TNBS)and 50%ethanol at 2:1(volume ratio).After the model was successfully prepared,rats in the herbal cake-partitioned moxibustion group received herbal cake-partitioned moxibustion at Qihai(CV 6)and bilateral Tianshu(ST 25).Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat colon;immunohistochemical technique was used to detect the expression of colonic CD206 protein;Western blot,immuno fluorescence,and real-time fluorescence quantitative polymerase chain reaction(RTqPCR)technologies were used to detect the protein and mRNA expressions of colonic AMPK and TSC2.Results:Compared with the normal group,rats in the model group showed damaged colonic mucosa,missing of the epithelial layer;thickened submucosa,vascular proliferation,massive infiltration of monocytes and lymphocytes,and cracked ulcers that reached the muscle layer.Rats in the herbal cake-partitioned moxibustion group showed reduced intestinal inflammation and healing intestinal epithelium ulcers.Compared with the normal group,rat colonic CD206 protein expression,and the protein and mRNA expressions of colonic AMPK and TSC2 were decreased in the model group(all P<0.Ol);compared with the model group,rat colonic CD206 protei n expression was in creased(P<Q.Q1),as well as the protein and mRNA expressions of AMPK and TSC2 in the herbal cake-partitioned moxibustion(all P<0.05).Conclusion:Herbal cake-partitioned moxibustion can reduce intestinal inflammation in CD rats,increase colonic CD206 protein expression,and up-regulate the protein and mRNA expressions of colonic AMPK and TSC2.