Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model...Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.展开更多
Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, subst...Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors. Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu （ST25） and Qihai （CV6） points were selected for herb-partitioned moxi- bustion. Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance E and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.展开更多
OBJECTIVE: To investigate the effects of warming moxibustion Tianshu(ST 25, bilateral) and Qihai(CV6) in patients with diarrhea-dominant irritable bowel syndrome(D-IBS) by assessing predominant factors, and determinin...OBJECTIVE: To investigate the effects of warming moxibustion Tianshu(ST 25, bilateral) and Qihai(CV6) in patients with diarrhea-dominant irritable bowel syndrome(D-IBS) by assessing predominant factors, and determining the best factor combinations and their interactions. To identify the optimal quantity of warming moxibustion Tianshu(ST 25, bilateral) and Qihai(CV 6) to achieve optimal efficacy.METHODS: An L9(3~4) orthogonal design was applied to 233 confirmed subjects based on the three most influential factors and the three most frequently used levels. Nine programs were designed.Subjects were assigned to four subgroups according to age and gender; each subject underwent one of the nine programs randomly. We selected Tianshu(ST 25, bilateral) and Qihai(CV 6). The scores of symptoms and the IBS Quality of Life(IBS-QOL) were evaluated after the final treatment.RESULTS: After treatment, clinical symptoms of all patients were significantly improved(P = 0.001).Quality of life also improved significantly(P < 0.05).For all the participants, frequency was the dominating factor regarding symptoms(P = 0.01). Duration was the dominating factor for quality of life(P =0.0001). The best combination for improving symptoms in 30-40-year-old male and female patients was two courses of 10 min each once a day, and for41-50-year-old male and female patients it was three courses of 10 min(males) or 30 min(females)twice a day. The best combination for quality of life was three courses of 20 min once a day. No interaction was found between frequency and course, or frequency and duration.CONCLUSION: Warming moxibustion Tianshu(ST25, bilateral) and Qihai(CV 6) is a promising therapy for D-IBS. To acquire optimal efficacy, the three main factors(duration, frequency and treatment course) of the warming moxibustion should reach a specific quantity and be combined appropriately.展开更多
Objective To verify the therapeutic effect of abdominal acupuncture combined with scarring moxibustion at Qìhǎi（气海 CV 6） on periarthritis of shoulder.Methods Thirty cases of periarthritis of shoulder were ra...Objective To verify the therapeutic effect of abdominal acupuncture combined with scarring moxibustion at Qìhǎi（气海 CV 6） on periarthritis of shoulder.Methods Thirty cases of periarthritis of shoulder were randomly divided into an acup-moxibustion group and an acupuncture group,15 cases in each group.The patients in the two groups were treated with abdominal acupuncture at Zhōngwǎn（中脘 CV 12）,Xiàwǎn（下脘 CV 10）,Shāngqǔ（商曲 KI 17）,Huáròumén（滑肉门 ST 24）,with scarring moxibustion at Qìhǎi（气海 CV 6） added in the acup-moxibustion group,5 treatments each week,altogether 15 treatments.The therapeutic effects were assessed on the 20th day of acupuncture.Results The effective rate was 93.3%（14/15） in both the acup-moxibustion group and the acupuncture group,and the cured and markedly effective rate in the acup-moxibustion group and the acupuncture group was 86.7%（13/15） and 53.3%（8/15）,respectively,the acup-moxibustion group being better than that in the acupuncture group（P〈0.01）;after treatment the cumulative score of pain and the cumulative score of shoulder joint active function were significantly increased in the two groups（all P〈0.05）,with more significantly increased in the acup-moxibustion group（both P〈0.05）.Conclusion The therapeutic effect of abdominal acupuncture combined with scarring moxibustion on periarthritis of shoulder is better than that of the simple abdominal acupuncture.展开更多
Objective： To observe the effect of postoperative urinary retention treated with tuina therapy. Methods： Tuina therapy was applied on 37 cases of postoperative urinary retention; Qihai （CV 6）, Guanyuan （CV 4）, Z...Objective： To observe the effect of postoperative urinary retention treated with tuina therapy. Methods： Tuina therapy was applied on 37 cases of postoperative urinary retention; Qihai （CV 6）, Guanyuan （CV 4）, Zhongji （CV 3） and bilateral Sanyinjiao （SP 6） were selected. Results： Thirty-six cases were cured and 1 case was not surveyed after 1 to 3 treatments. Conclusion： Point tuina is an effective method in treating postoperative urinary retention.展开更多
OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine r...OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.展开更多
Objective： To observe the regulatory effects of herbal cake-partitioned moxibustion on Crohn＇s disease （CD） rat＇s colon inflammatory cytokine interleukin-17 （IL-17）, IL-23 and their mRNAs, and to investigate th...Objective： To observe the regulatory effects of herbal cake-partitioned moxibustion on Crohn＇s disease （CD） rat＇s colon inflammatory cytokine interleukin-17 （IL-17）, IL-23 and their mRNAs, and to investigate the action mechanism of moxibustion in treating CD. Methods： Forty SPF grade Sprague-Dauley （SD） male rats were randomly divided into a normal group, a model group, an herbal cake-partitioned moxibustion group and a Western medicine group, with 20 rats in each group. Except the normal group, rats in the other three groups were used to make CD model by giving an enema in colon with Trinitro-benzene-sulfonic acid （TNBS）. When the models were successful made, rats in the model group had no therapeutic intervention; rats in the herbal cake-partitioned moxibustion group were subjected to herbal cake-partitioned moxibustion treatment at bilateral Tianshu （ST 25） plus O.ihai （CV 6）; and rats in the Western medicine group were given oral Mesalazine. After treatment, the histopathological changes and inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were observed in descending colon by hematoxylin-eosin （HE） staining, immunohistochemistry （IHC） and real-time polymerase chain reaction （RT-PCR） methods. Results： Colon tissues of TNBS enema rat models showed cracks-like ulcers accompanied by mucous layer inflammation, granulomas, and inflammatory cytokines IL-27, IL-23 and their mRNAs expressions were all higher than those in the normal group （P〈O.01）; after intervention, colon tissue cracks-like ulcers and inflammation degree reduced, inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were all significantly decreased （P〈0.01） in the herbal cake-partitioned moxibustion group and the Western medicine group. Conclusion： Herbal cake-partitioned moxibustion may improve colon tissue ulcers and relieve intestinal inflammation by down-regulation of IL-17, IL-23 and their mRNAs expressions in CD model rats＇ colonic mucosa.展开更多
Objective： To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion （RPE） in athletes with exercise-induced fatigue. Methods： A total of 35 football players were rand...Objective： To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion （RPE） in athletes with exercise-induced fatigue. Methods： A total of 35 football players were randomly allocated into a blank group, a control group and an observation group. The athletes in the blank group received no treatment, those in the control group take Xi Yang Shen （Radix Panacis Quinquefolii） orally and those in the observation group were treated by point sticking therapy. The athletes＇ RPE, hemoglobin （Hb）, red blood cell count （RBC）, blood urea nitrogen （BUN） and blood glucose （BG） were recorded every day for analysis. Results： The RPE value in the blank group tends to increase, while that in the treatment and the blank groups tend to increase first but decrease afterwards, and the corresponding RPE value in the observation group was smaller than that in the control group. After treatment, athletes in the blank group showed a decrease in Hb, RBC and BG, but an increase in BUN in the blank group; while athletes in the observation and the control groups showed increases in Hb, RBC and BG, but a decrease in BUN. Conclusion： Point sticking can alleviate clinical symptoms induced by exercise-induced fatigue.展开更多
Objective： To observe the effect of moxibustion on the protein and mRNA expressions of corticotropin-releasing factor （CRF） and corticotropin-releasing factor receptor 2 （CRFR1） in hypothalamus of trinitrobenzene...Objective： To observe the effect of moxibustion on the protein and mRNA expressions of corticotropin-releasing factor （CRF） and corticotropin-releasing factor receptor 2 （CRFR1） in hypothalamus of trinitrobenzene sulfonic acid （TNBS）-induced experimental colitis rats, and to explore the central mechanisms of moxibustion in improving visceral pain and the pain-related emotions in experimental colitis rats. Methods： Thirty-six Sprague-Dawley （SD） rats were randomly divided into a normal group （NG）, a model group （MG）, a herb-partitioned moxibustion group （HPMG） and a sham herb-partitioned moxibustion group （SHPMG）. Except the NG, rats in the remaining three groups all received TNBS enema to establish experimental colitis models. The HPMG received herb-partitioned moxibustion （HPM） at bilateral Tianshu （ST 25） and Qihai （CV 6） for intervention; for the SHPMG, the herbal cakes and moxa cones were only placed on the acupoints but not ignited; rats in the MG and NG were only fixed in the same way as those in the HPMG but did not receive any treatment. At the end of the intervention, the abdominal withdrawal reflex （AWR） score, the open field test （OFT） score and the elevated plus maze （EPM） score were observed to measure the changes in visceral pain and pain-related emotions of the rats. The enzyme-linked immunosorbent assay （ELISA） was used to examine the expressions of CRF and CRFR1 proteins in hypothalamus; the fluorescence-based quantitative polymerase chain reaction （PCR） was used to detect the expressions of CRF and CRFR1 mRNAs in hypothalamus. Results： Compared with the NG, the AWR score increased significantly and the OFT and EPM scores dropped significantly in the MG （all P〈0.05）, and the expressions of hypothalamic CRF and CRFR1 proteins and mRNAs increased significantly （all P〈0.01）. Compared with the MG and SHPMG, the AWR score dropped significantly and the OFT and EPM scores increased significantly in the HPMG （all P〈0.01）, and the expressions of hypothalamic CRF and CRFRI proteins and mRNAs decreased significantly （all P〈0.05）. There were no significant differences between the MG and the SHPMG （all P〉0.05）. Conclusion： HPM can down-regulate the abnormally increased expressions of CRF and CRFR1 proteins and mRNAs in hypothalamus of the TNBS-induced experimental colitis rats, which is plausibly one of its action mechanisms in mitigating visceral pain and the pain-related emotions in the experimental colitis rats.展开更多
Objective:To explore the mechanism of herbal cake-partitioned moxibustion in Crohn disease(CD)treatment by observing the effect of herbal cake-partitioned moxibustion on protein expressions of colonic M2 macrophage ma...Objective:To explore the mechanism of herbal cake-partitioned moxibustion in Crohn disease(CD)treatment by observing the effect of herbal cake-partitioned moxibustion on protein expressions of colonic M2 macrophage marker CD206,AMP-activated protein kinase(AMPK)and tuberous sclerosis complex(TSC)2.Methods:Twenty-six specific pathogen free male rats were randomly divided into a normal group,a model group and a herbal cake-partitioned moxibustion group.The CD model was prepared by enema with the mixture of 5%(W/V)2,4,6-trinitrobenzene sulfonic acid(TNBS)and 50%ethanol at 2:1(volume ratio).After the model was successfully prepared,rats in the herbal cake-partitioned moxibustion group received herbal cake-partitioned moxibustion at Qihai(CV 6)and bilateral Tianshu(ST 25).Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat colon;immunohistochemical technique was used to detect the expression of colonic CD206 protein;Western blot,immuno fluorescence,and real-time fluorescence quantitative polymerase chain reaction(RTqPCR)technologies were used to detect the protein and mRNA expressions of colonic AMPK and TSC2.Results:Compared with the normal group,rats in the model group showed damaged colonic mucosa,missing of the epithelial layer;thickened submucosa,vascular proliferation,massive infiltration of monocytes and lymphocytes,and cracked ulcers that reached the muscle layer.Rats in the herbal cake-partitioned moxibustion group showed reduced intestinal inflammation and healing intestinal epithelium ulcers.Compared with the normal group,rat colonic CD206 protein expression,and the protein and mRNA expressions of colonic AMPK and TSC2 were decreased in the model group(all P<0.Ol);compared with the model group,rat colonic CD206 protei n expression was in creased(P<Q.Q1),as well as the protein and mRNA expressions of AMPK and TSC2 in the herbal cake-partitioned moxibustion(all P<0.05).Conclusion:Herbal cake-partitioned moxibustion can reduce intestinal inflammation in CD rats,increase colonic CD206 protein expression,and up-regulate the protein and mRNA expressions of colonic AMPK and TSC2.展开更多
基金supported by the National Natural Science Foundation of China,No.14202225,&.81574042the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China,No.2000202a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China,No.JDZX2015314
文摘Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.
基金supported by the National Natural Science Foundation of China,No.81273843,81674073a grant from the National Key Basic Research Program of China(973 Program)+1 种基金No.2015CB554501the Project Fund of Shanghai Municipal Commission of Health and Family Planning of China,No.20144Y0153,2017BR047
文摘Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors. Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu （ST25） and Qihai （CV6） points were selected for herb-partitioned moxi- bustion. Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance E and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.
基金Supported by the National Basic Research Program of China(973 Program):Research on Basic Theory and Regularity of Moxibustion(No.2009CB522900)the National Natural Science Foundation of China:Involvement of Sigma-1 Receptor in Analgesic of Moxibustion on Chronic Visceral Hyperalgesia in Irritable bowel syndrome(IBS)-like Rats(No.81303031)+3 种基金the National Natural Science Foundation of China:Study on the Mechanism of Moxibustion Effect on Reconstruction of Post Infectious(PI)-IBS Intestinal Homeostasis via Intestinal Microbiota-Mucosal Immunity(No.81503656)Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function:Effect of Needle Warming Moxibustion on Clinical Effect and Psychological Condition for Female D-IBS(No.14DZ2260500)Shanghai Municipal Commission of Health and Family Planning:Observation on the Curative Effect and Ovarian Hormone Levels as Well as Psychological Condition of Female D-IBS Patients in Child-bearing Stage by Acupuncture&Moxibustion(No.20164Y0151)the Shanghai Sailing Program:and the Mechanism of Central Response in the Treatment of Diarrhea-predominate Irritable Bowel Syndrome by Moxibustion(No.15YF1411200)
文摘OBJECTIVE: To investigate the effects of warming moxibustion Tianshu(ST 25, bilateral) and Qihai(CV6) in patients with diarrhea-dominant irritable bowel syndrome(D-IBS) by assessing predominant factors, and determining the best factor combinations and their interactions. To identify the optimal quantity of warming moxibustion Tianshu(ST 25, bilateral) and Qihai(CV 6) to achieve optimal efficacy.METHODS: An L9(3~4) orthogonal design was applied to 233 confirmed subjects based on the three most influential factors and the three most frequently used levels. Nine programs were designed.Subjects were assigned to four subgroups according to age and gender; each subject underwent one of the nine programs randomly. We selected Tianshu(ST 25, bilateral) and Qihai(CV 6). The scores of symptoms and the IBS Quality of Life(IBS-QOL) were evaluated after the final treatment.RESULTS: After treatment, clinical symptoms of all patients were significantly improved(P = 0.001).Quality of life also improved significantly(P < 0.05).For all the participants, frequency was the dominating factor regarding symptoms(P = 0.01). Duration was the dominating factor for quality of life(P =0.0001). The best combination for improving symptoms in 30-40-year-old male and female patients was two courses of 10 min each once a day, and for41-50-year-old male and female patients it was three courses of 10 min(males) or 30 min(females)twice a day. The best combination for quality of life was three courses of 20 min once a day. No interaction was found between frequency and course, or frequency and duration.CONCLUSION: Warming moxibustion Tianshu(ST25, bilateral) and Qihai(CV 6) is a promising therapy for D-IBS. To acquire optimal efficacy, the three main factors(duration, frequency and treatment course) of the warming moxibustion should reach a specific quantity and be combined appropriately.
文摘Objective To verify the therapeutic effect of abdominal acupuncture combined with scarring moxibustion at Qìhǎi（气海 CV 6） on periarthritis of shoulder.Methods Thirty cases of periarthritis of shoulder were randomly divided into an acup-moxibustion group and an acupuncture group,15 cases in each group.The patients in the two groups were treated with abdominal acupuncture at Zhōngwǎn（中脘 CV 12）,Xiàwǎn（下脘 CV 10）,Shāngqǔ（商曲 KI 17）,Huáròumén（滑肉门 ST 24）,with scarring moxibustion at Qìhǎi（气海 CV 6） added in the acup-moxibustion group,5 treatments each week,altogether 15 treatments.The therapeutic effects were assessed on the 20th day of acupuncture.Results The effective rate was 93.3%（14/15） in both the acup-moxibustion group and the acupuncture group,and the cured and markedly effective rate in the acup-moxibustion group and the acupuncture group was 86.7%（13/15） and 53.3%（8/15）,respectively,the acup-moxibustion group being better than that in the acupuncture group（P〈0.01）;after treatment the cumulative score of pain and the cumulative score of shoulder joint active function were significantly increased in the two groups（all P〈0.05）,with more significantly increased in the acup-moxibustion group（both P〈0.05）.Conclusion The therapeutic effect of abdominal acupuncture combined with scarring moxibustion on periarthritis of shoulder is better than that of the simple abdominal acupuncture.
文摘Objective： To observe the effect of postoperative urinary retention treated with tuina therapy. Methods： Tuina therapy was applied on 37 cases of postoperative urinary retention; Qihai （CV 6）, Guanyuan （CV 4）, Zhongji （CV 3） and bilateral Sanyinjiao （SP 6） were selected. Results： Thirty-six cases were cured and 1 case was not surveyed after 1 to 3 treatments. Conclusion： Point tuina is an effective method in treating postoperative urinary retention.
基金Supported by the National Basic Research Program of China(973 Program,No.2015CB554501)Program of National Natural Science Foundation of China(No.81574079)+2 种基金Program for outstanding medical academic leader(2015,No.80)Shuguang Program of Shanghai Education Commission(No.14SG39)Shanghai Rising-Star Program(No.16QA1403400)
文摘OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.
基金supported by National Basic Research Program of China(973 Program,No.2015CB554501)Natural Science Foundation of Shanghai(No.13ZR1439400)+1 种基金Program for New Century Excellent Talents in University(No.NCET-13-0907)Leading Academic Discipline Project of Shanghai(No.JZ2012019)~~
文摘Objective： To observe the regulatory effects of herbal cake-partitioned moxibustion on Crohn＇s disease （CD） rat＇s colon inflammatory cytokine interleukin-17 （IL-17）, IL-23 and their mRNAs, and to investigate the action mechanism of moxibustion in treating CD. Methods： Forty SPF grade Sprague-Dauley （SD） male rats were randomly divided into a normal group, a model group, an herbal cake-partitioned moxibustion group and a Western medicine group, with 20 rats in each group. Except the normal group, rats in the other three groups were used to make CD model by giving an enema in colon with Trinitro-benzene-sulfonic acid （TNBS）. When the models were successful made, rats in the model group had no therapeutic intervention; rats in the herbal cake-partitioned moxibustion group were subjected to herbal cake-partitioned moxibustion treatment at bilateral Tianshu （ST 25） plus O.ihai （CV 6）; and rats in the Western medicine group were given oral Mesalazine. After treatment, the histopathological changes and inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were observed in descending colon by hematoxylin-eosin （HE） staining, immunohistochemistry （IHC） and real-time polymerase chain reaction （RT-PCR） methods. Results： Colon tissues of TNBS enema rat models showed cracks-like ulcers accompanied by mucous layer inflammation, granulomas, and inflammatory cytokines IL-27, IL-23 and their mRNAs expressions were all higher than those in the normal group （P〈O.01）; after intervention, colon tissue cracks-like ulcers and inflammation degree reduced, inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were all significantly decreased （P〈0.01） in the herbal cake-partitioned moxibustion group and the Western medicine group. Conclusion： Herbal cake-partitioned moxibustion may improve colon tissue ulcers and relieve intestinal inflammation by down-regulation of IL-17, IL-23 and their mRNAs expressions in CD model rats＇ colonic mucosa.
基金supported by Boom Plan Project of Shanghai Municipal Sports Bureau(07TF005)Boom Plan Project of Shanghai Sports Science and Technology(09TF020)+1 种基金Project of Shanghai Science and Technology Committee(10DZ1973800)Shanghai Key Discipline Construction Project(S30304)
文摘Objective： To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion （RPE） in athletes with exercise-induced fatigue. Methods： A total of 35 football players were randomly allocated into a blank group, a control group and an observation group. The athletes in the blank group received no treatment, those in the control group take Xi Yang Shen （Radix Panacis Quinquefolii） orally and those in the observation group were treated by point sticking therapy. The athletes＇ RPE, hemoglobin （Hb）, red blood cell count （RBC）, blood urea nitrogen （BUN） and blood glucose （BG） were recorded every day for analysis. Results： The RPE value in the blank group tends to increase, while that in the treatment and the blank groups tend to increase first but decrease afterwards, and the corresponding RPE value in the observation group was smaller than that in the control group. After treatment, athletes in the blank group showed a decrease in Hb, RBC and BG, but an increase in BUN in the blank group; while athletes in the observation and the control groups showed increases in Hb, RBC and BG, but a decrease in BUN. Conclusion： Point sticking can alleviate clinical symptoms induced by exercise-induced fatigue.
文摘Objective： To observe the effect of moxibustion on the protein and mRNA expressions of corticotropin-releasing factor （CRF） and corticotropin-releasing factor receptor 2 （CRFR1） in hypothalamus of trinitrobenzene sulfonic acid （TNBS）-induced experimental colitis rats, and to explore the central mechanisms of moxibustion in improving visceral pain and the pain-related emotions in experimental colitis rats. Methods： Thirty-six Sprague-Dawley （SD） rats were randomly divided into a normal group （NG）, a model group （MG）, a herb-partitioned moxibustion group （HPMG） and a sham herb-partitioned moxibustion group （SHPMG）. Except the NG, rats in the remaining three groups all received TNBS enema to establish experimental colitis models. The HPMG received herb-partitioned moxibustion （HPM） at bilateral Tianshu （ST 25） and Qihai （CV 6） for intervention; for the SHPMG, the herbal cakes and moxa cones were only placed on the acupoints but not ignited; rats in the MG and NG were only fixed in the same way as those in the HPMG but did not receive any treatment. At the end of the intervention, the abdominal withdrawal reflex （AWR） score, the open field test （OFT） score and the elevated plus maze （EPM） score were observed to measure the changes in visceral pain and pain-related emotions of the rats. The enzyme-linked immunosorbent assay （ELISA） was used to examine the expressions of CRF and CRFR1 proteins in hypothalamus; the fluorescence-based quantitative polymerase chain reaction （PCR） was used to detect the expressions of CRF and CRFR1 mRNAs in hypothalamus. Results： Compared with the NG, the AWR score increased significantly and the OFT and EPM scores dropped significantly in the MG （all P〈0.05）, and the expressions of hypothalamic CRF and CRFR1 proteins and mRNAs increased significantly （all P〈0.01）. Compared with the MG and SHPMG, the AWR score dropped significantly and the OFT and EPM scores increased significantly in the HPMG （all P〈0.01）, and the expressions of hypothalamic CRF and CRFRI proteins and mRNAs decreased significantly （all P〈0.05）. There were no significant differences between the MG and the SHPMG （all P〉0.05）. Conclusion： HPM can down-regulate the abnormally increased expressions of CRF and CRFR1 proteins and mRNAs in hypothalamus of the TNBS-induced experimental colitis rats, which is plausibly one of its action mechanisms in mitigating visceral pain and the pain-related emotions in the experimental colitis rats.
文摘Objective:To explore the mechanism of herbal cake-partitioned moxibustion in Crohn disease(CD)treatment by observing the effect of herbal cake-partitioned moxibustion on protein expressions of colonic M2 macrophage marker CD206,AMP-activated protein kinase(AMPK)and tuberous sclerosis complex(TSC)2.Methods:Twenty-six specific pathogen free male rats were randomly divided into a normal group,a model group and a herbal cake-partitioned moxibustion group.The CD model was prepared by enema with the mixture of 5%(W/V)2,4,6-trinitrobenzene sulfonic acid(TNBS)and 50%ethanol at 2:1(volume ratio).After the model was successfully prepared,rats in the herbal cake-partitioned moxibustion group received herbal cake-partitioned moxibustion at Qihai(CV 6)and bilateral Tianshu(ST 25).Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat colon;immunohistochemical technique was used to detect the expression of colonic CD206 protein;Western blot,immuno fluorescence,and real-time fluorescence quantitative polymerase chain reaction(RTqPCR)technologies were used to detect the protein and mRNA expressions of colonic AMPK and TSC2.Results:Compared with the normal group,rats in the model group showed damaged colonic mucosa,missing of the epithelial layer;thickened submucosa,vascular proliferation,massive infiltration of monocytes and lymphocytes,and cracked ulcers that reached the muscle layer.Rats in the herbal cake-partitioned moxibustion group showed reduced intestinal inflammation and healing intestinal epithelium ulcers.Compared with the normal group,rat colonic CD206 protein expression,and the protein and mRNA expressions of colonic AMPK and TSC2 were decreased in the model group(all P<0.Ol);compared with the model group,rat colonic CD206 protei n expression was in creased(P<Q.Q1),as well as the protein and mRNA expressions of AMPK and TSC2 in the herbal cake-partitioned moxibustion(all P<0.05).Conclusion:Herbal cake-partitioned moxibustion can reduce intestinal inflammation in CD rats,increase colonic CD206 protein expression,and up-regulate the protein and mRNA expressions of colonic AMPK and TSC2.
