Objective To evaluate the clinical efficacy and safety of Zhenzhu Qingyuan Granules through a clinical randomized controlled trial and to analyze the potential action targets and pathways of this formula using network...Objective To evaluate the clinical efficacy and safety of Zhenzhu Qingyuan Granules through a clinical randomized controlled trial and to analyze the potential action targets and pathways of this formula using network pharmacology.Methods Patients with gastroesophageal reflux disease(GERD)of liver–stomach stagnant heat pattern who met the inclusion and exclusion criteria were randomly divided into the control group and the observation group.The control group received oral rabeprazole,whereas the observation group were given Zhenzhu Qingyuan Granules in addition to the rabeprazole.The treatment duration was 8 weeks.Clinical efficacy was observed in both groups after 8 weeks.Network pharmacology was used to analyze the action targets of ZhenzhuQingyuanGranules and the genes related to GERD,and core targets were inferred.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted to explore the potential mechanisms of this formula.Results The clinical research results showed that the total effective rate in the treatment group was 92.68%,compared with 70.00%in the control group,with a statistically significant difference(p<0.05).After treatment,both Chinese medicine syndrome score and endoscopic score improved in both groups compared with before treatment(p<0.05),and the treatment group showed greater improvement than the control group(p<0.05).Network pharmacology identified effective components of Zhenzhu Qingyuan Granules for treating GERD,including quercetin,luteolin,andβ-sitosterol,with potential action targets such as tumor protein 53(TP53),protein kinase B(AKT1),and tumor necrosis factor.Conclusion Zhenzhu Qingyuan Granules can significantly improve clinical symptoms in patients with GERD of liver–stomach stagnated heat pattern,enhance clinical efficacy,and have high safety.This formula may exert therapeutic effects through multiple targets and pathways.展开更多
Qingyuan County, Zhejiang, China is recognized as a source of mushrooms around the world and as Mushroom City in China. Because of a favorable ecological environment and abundant forest resources, mushroom farmers in ...Qingyuan County, Zhejiang, China is recognized as a source of mushrooms around the world and as Mushroom City in China. Because of a favorable ecological environment and abundant forest resources, mushroom farmers in Qingyuan have engaged in mushroom production for 800 years and continued to form unique mushroom production systems where people and nature live in a harmonious way, and a rich and colorful mushroom culture. The Qingyuan Mushroom Culture System was named the Nationally Important Agricultural Heritage systems of China in 2014. Based on analysis of the importance of agricultural heritage and the necessity and urgency of pro-tection, here we examine the agricultural heritage of the Qingyuan mushroom culture system as a research case study and comprehensively describe its heritage values, including supply materials and production, ecosystem ser-vices, cultural inheritance and multi-function agricultural development. These results provide reference values for agricultural heritage protection and inheritance of edible fungi in China, agricultural sustainable development and extension of agricultural function.展开更多
基金National Administration of Traditional Chinese Medicine Evidence-Based Capacity Building Project(2019XZZXXH005)Special Project on Traditional Chinese Medicine Scientific Research of Health Commission of Henan Province(2022ZY2022)+1 种基金Henan Provincial Top Talents Cultivation Project in Traditional Chinese Medicine Discipline of Henan Provincial Traditional Chinese Medicine Inheritance and Innovation Talents Project(Zhongjing Project)(Henan Health TraditionalMedicine Letter[2021]No.15)Special Project on Traditional Chinese Medicine Scientific Research of Health Commission of Henan Province(2023ZY2062).
文摘Objective To evaluate the clinical efficacy and safety of Zhenzhu Qingyuan Granules through a clinical randomized controlled trial and to analyze the potential action targets and pathways of this formula using network pharmacology.Methods Patients with gastroesophageal reflux disease(GERD)of liver–stomach stagnant heat pattern who met the inclusion and exclusion criteria were randomly divided into the control group and the observation group.The control group received oral rabeprazole,whereas the observation group were given Zhenzhu Qingyuan Granules in addition to the rabeprazole.The treatment duration was 8 weeks.Clinical efficacy was observed in both groups after 8 weeks.Network pharmacology was used to analyze the action targets of ZhenzhuQingyuanGranules and the genes related to GERD,and core targets were inferred.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted to explore the potential mechanisms of this formula.Results The clinical research results showed that the total effective rate in the treatment group was 92.68%,compared with 70.00%in the control group,with a statistically significant difference(p<0.05).After treatment,both Chinese medicine syndrome score and endoscopic score improved in both groups compared with before treatment(p<0.05),and the treatment group showed greater improvement than the control group(p<0.05).Network pharmacology identified effective components of Zhenzhu Qingyuan Granules for treating GERD,including quercetin,luteolin,andβ-sitosterol,with potential action targets such as tumor protein 53(TP53),protein kinase B(AKT1),and tumor necrosis factor.Conclusion Zhenzhu Qingyuan Granules can significantly improve clinical symptoms in patients with GERD of liver–stomach stagnated heat pattern,enhance clinical efficacy,and have high safety.This formula may exert therapeutic effects through multiple targets and pathways.
基金Consulting Project of the Chinese Academy of Engineering(2013-XZ-22)
文摘Qingyuan County, Zhejiang, China is recognized as a source of mushrooms around the world and as Mushroom City in China. Because of a favorable ecological environment and abundant forest resources, mushroom farmers in Qingyuan have engaged in mushroom production for 800 years and continued to form unique mushroom production systems where people and nature live in a harmonious way, and a rich and colorful mushroom culture. The Qingyuan Mushroom Culture System was named the Nationally Important Agricultural Heritage systems of China in 2014. Based on analysis of the importance of agricultural heritage and the necessity and urgency of pro-tection, here we examine the agricultural heritage of the Qingyuan mushroom culture system as a research case study and comprehensively describe its heritage values, including supply materials and production, ecosystem ser-vices, cultural inheritance and multi-function agricultural development. These results provide reference values for agricultural heritage protection and inheritance of edible fungi in China, agricultural sustainable development and extension of agricultural function.
