[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroeso...[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroesophageal reflux disease (GERD).[Methods] Twenty-four SPF SD rats were randomly divided into control group, model group, Chinese medicine group and western medicine group, with 6 rats in each group. Except the control group, the other three groups were used to establish the rat model of GERD combined with IPF by injecting hydrochloric acid into the lower end of esophagus and inhaling diluted bleomycin (5 mg/kg). Rats in the Chinese medicine group (14 g/kg), rats in the western medicine group (4.17 g/kg), rats in the control group and the model group were given the same volume of saline by gavage for 14 d. Morphological and pathological changes of esophageal and lung tissues were observed under light microscope, and T lymphocyte subsets (CD + 3, CD + 4, CD + 8) and the ratio of CD + 4/CD + 8 in peripheral blood were detected by flow cytometry.[Results] Compared with the control group, the pulmonary tissue of the model group showed that the pulmonary interstitium was obviously thickened, the alveoli were mutually fused, the structure was obviously destroyed, the original alveolar structure was disappeared, the inflammatory cell infiltration was around the pulmonary capillaries and the alveolar space, and the basal cell hyperplasia and inflammatory cell infiltration were at the lower end of the esophagus. Compared with the model group, the degree of inflammatory cell infiltration and lung tissue damage in the Chinese medicine group and the western medicine group was significantly reduced, the inflammatory infiltration in the lower esophagus was significantly reduced, and the cell proliferation was reduced. Compared with the control group, the CD + 3, CD + 4, CD + 8, CD + 4/CD + 8 in the peripheral blood of the rats in the model group, the Chinese medicine group and the western medicine group decreased ( P <0.01). Compared with the model group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01), CD + 8 decreased ( P >0.05). Compared with the Chinese medicine group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01) and CD + 8 decreased ( P >0.05) in the western medicine group.[Conclusions] Qishi Shengjiang Guiyuan Granules can effectively improve the inflammation of the lower esophagus and lung tissues of the pulmonary fibrosis rats with GERD and IFP, and regulate the number of T lymphocyte subsets CD + 3, CD + 4, CD + 8 cells and CD + 4/CD + 8 ratio in peripheral blood.展开更多
目的:探讨芪实颗粒联合盆底肌训练对女性轻中度压力性尿失禁患者尿失禁问卷表简表(international consultation on incontinence questionnaire urinary incontinence short form,ICI-Q-SF)评分、尿失禁生活质量量表(incontinence quali...目的:探讨芪实颗粒联合盆底肌训练对女性轻中度压力性尿失禁患者尿失禁问卷表简表(international consultation on incontinence questionnaire urinary incontinence short form,ICI-Q-SF)评分、尿失禁生活质量量表(incontinence quality of life instrument,I-QOL)评分的影响。方法:选取2014年10月至2017年10月中国中医科学院西苑医院收治的女性轻中度压力性尿失禁患者62例,以随机数字表法分为观察组和对照组,每组31例。观察组患者给予芪实颗粒,对照组患者给予安慰剂颗粒,两组患者服药的同时均行盆底肌训练,共观察8周。观察两组患者不同时间的ICI-Q-SF评分、I-QOL评分。结果:治疗6、8周后,观察组患者的ICI-Q-SF评分明显低于对照组,差异均有统计学意义( P <0.05);治疗4、6及8周后,观察组患者的I-QOL评分明显高于对照组,差异均有统计学意义( P <0.05);观察组患者治疗前后ICI-Q-SF评分、I-QOL评分变化值分别为(4.58±2.53)、(13.19±7.69)分,明显高于对照组的(3.06±1.82)、(5.10±4.71)分,差异均有统计学意义( P <0.05)。结论:芪实颗粒联合盆底肌训练可改善女性轻中度压力性尿失禁患者的ICI-Q-SF评分及I-QOL评分,疗效优于仅采用盆底肌训练。展开更多
基金Supported by Young and Middle-aged Teachers Scientific Research Basic Ability Promotion Project in Universities of Guangxi(2021 KY0309)Self-funded Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYZ20210266)+1 种基金School-level Scientific Research Project of Guangxi University of Chinese Medicine(2021QN016)Hospital-level Scientific Research Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine(2020QN001).
文摘[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroesophageal reflux disease (GERD).[Methods] Twenty-four SPF SD rats were randomly divided into control group, model group, Chinese medicine group and western medicine group, with 6 rats in each group. Except the control group, the other three groups were used to establish the rat model of GERD combined with IPF by injecting hydrochloric acid into the lower end of esophagus and inhaling diluted bleomycin (5 mg/kg). Rats in the Chinese medicine group (14 g/kg), rats in the western medicine group (4.17 g/kg), rats in the control group and the model group were given the same volume of saline by gavage for 14 d. Morphological and pathological changes of esophageal and lung tissues were observed under light microscope, and T lymphocyte subsets (CD + 3, CD + 4, CD + 8) and the ratio of CD + 4/CD + 8 in peripheral blood were detected by flow cytometry.[Results] Compared with the control group, the pulmonary tissue of the model group showed that the pulmonary interstitium was obviously thickened, the alveoli were mutually fused, the structure was obviously destroyed, the original alveolar structure was disappeared, the inflammatory cell infiltration was around the pulmonary capillaries and the alveolar space, and the basal cell hyperplasia and inflammatory cell infiltration were at the lower end of the esophagus. Compared with the model group, the degree of inflammatory cell infiltration and lung tissue damage in the Chinese medicine group and the western medicine group was significantly reduced, the inflammatory infiltration in the lower esophagus was significantly reduced, and the cell proliferation was reduced. Compared with the control group, the CD + 3, CD + 4, CD + 8, CD + 4/CD + 8 in the peripheral blood of the rats in the model group, the Chinese medicine group and the western medicine group decreased ( P <0.01). Compared with the model group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01), CD + 8 decreased ( P >0.05). Compared with the Chinese medicine group, CD + 3, CD + 4, and CD + 4/CD + 8 increased ( P >0.05, P >0.05, P <0.01) and CD + 8 decreased ( P >0.05) in the western medicine group.[Conclusions] Qishi Shengjiang Guiyuan Granules can effectively improve the inflammation of the lower esophagus and lung tissues of the pulmonary fibrosis rats with GERD and IFP, and regulate the number of T lymphocyte subsets CD + 3, CD + 4, CD + 8 cells and CD + 4/CD + 8 ratio in peripheral blood.
文摘目的:探讨芪实颗粒联合盆底肌训练对女性轻中度压力性尿失禁患者尿失禁问卷表简表(international consultation on incontinence questionnaire urinary incontinence short form,ICI-Q-SF)评分、尿失禁生活质量量表(incontinence quality of life instrument,I-QOL)评分的影响。方法:选取2014年10月至2017年10月中国中医科学院西苑医院收治的女性轻中度压力性尿失禁患者62例,以随机数字表法分为观察组和对照组,每组31例。观察组患者给予芪实颗粒,对照组患者给予安慰剂颗粒,两组患者服药的同时均行盆底肌训练,共观察8周。观察两组患者不同时间的ICI-Q-SF评分、I-QOL评分。结果:治疗6、8周后,观察组患者的ICI-Q-SF评分明显低于对照组,差异均有统计学意义( P <0.05);治疗4、6及8周后,观察组患者的I-QOL评分明显高于对照组,差异均有统计学意义( P <0.05);观察组患者治疗前后ICI-Q-SF评分、I-QOL评分变化值分别为(4.58±2.53)、(13.19±7.69)分,明显高于对照组的(3.06±1.82)、(5.10±4.71)分,差异均有统计学意义( P <0.05)。结论:芪实颗粒联合盆底肌训练可改善女性轻中度压力性尿失禁患者的ICI-Q-SF评分及I-QOL评分,疗效优于仅采用盆底肌训练。
