Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not cle...Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship （r2, 0.9994-0.9999）, precisions （inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%）, repeatability （RSD, 1.66%-3.67%）, stability （RSD, 1.26%-4.77%） and recovery （95.24%-105.41%, RSD, 0.29%-2.69%）. Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. 3＂he total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.展开更多
We present a specific-window method to subtract the interference of water vapor on terahertz frequency-domain spectroscopy（THz-FDS） at ambient temperature and pressure. A continuous-wave spectrometer based on photom...We present a specific-window method to subtract the interference of water vapor on terahertz frequency-domain spectroscopy（THz-FDS） at ambient temperature and pressure. A continuous-wave spectrometer based on photomixing was utilized to obtain THz-FDS of methanol vapor in the range of 50–1200 GHz. The distinctly spaced absorption features in the neighborhood of atmospheric windows of transparency were selected to perform linear fitting versus the calculated absorption cross section and obtain the concentration of methanol. Furthermore, the gradually decreased methanol vapor was quantified to demonstrate the reliability of the method.展开更多
The grinding of two parallel sides of a component is accomplished with theaccuracy and higher productivity by passing a blank through the truncated cone shape grinders, whichare turned angles. The machine is designate...The grinding of two parallel sides of a component is accomplished with theaccuracy and higher productivity by passing a blank through the truncated cone shape grinders, whichare turned angles. The machine is designated by the name of double disc grinding machine (DDGM).Usually, it is used in the mass production. The relationship between these angles, the accuracy,productivity, allowance and parameters of the machine and technology is explained in detail by math,such as vector analysis, transformation of 3D space coordinates, etc. Therefore, in the aspects ofqualitative and quantitative analyses, the grinding potential of DDGM is enormous increased andsuperior to conventional methods. Furthermore, the theoretical foundation of DDGM grinding designand technology is provided to improve, to expand and to create for future. The established machinedesign and practical experience of grinding technology will get great benefit by them.展开更多
基金Key Projects in the National Science & Technology Pillar Program during the 11thFive-Year Plan Period (Grant No.2008BAI51B02)National Natural Science Fund Project of China (Grant No. 81202904)
文摘Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship （r2, 0.9994-0.9999）, precisions （inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%）, repeatability （RSD, 1.66%-3.67%）, stability （RSD, 1.26%-4.77%） and recovery （95.24%-105.41%, RSD, 0.29%-2.69%）. Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. 3＂he total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.
基金supported by the China Postdoctoral Science Foundation(No.2017M610771)
文摘We present a specific-window method to subtract the interference of water vapor on terahertz frequency-domain spectroscopy（THz-FDS） at ambient temperature and pressure. A continuous-wave spectrometer based on photomixing was utilized to obtain THz-FDS of methanol vapor in the range of 50–1200 GHz. The distinctly spaced absorption features in the neighborhood of atmospheric windows of transparency were selected to perform linear fitting versus the calculated absorption cross section and obtain the concentration of methanol. Furthermore, the gradually decreased methanol vapor was quantified to demonstrate the reliability of the method.
文摘The grinding of two parallel sides of a component is accomplished with theaccuracy and higher productivity by passing a blank through the truncated cone shape grinders, whichare turned angles. The machine is designated by the name of double disc grinding machine (DDGM).Usually, it is used in the mass production. The relationship between these angles, the accuracy,productivity, allowance and parameters of the machine and technology is explained in detail by math,such as vector analysis, transformation of 3D space coordinates, etc. Therefore, in the aspects ofqualitative and quantitative analyses, the grinding potential of DDGM is enormous increased andsuperior to conventional methods. Furthermore, the theoretical foundation of DDGM grinding designand technology is provided to improve, to expand and to create for future. The established machinedesign and practical experience of grinding technology will get great benefit by them.
