Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia...Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia and the lack of glucose. Methods The experiments were performed in the culture of ECV 304 injured with hypoxia and the lack of glucose in vitro. The releases of intracellular lactate dehydrogenase(LDH) of ECV 304 was measured with automatic biochemistry analysis. NO level of ECV 304 was monitored with colorimetry. The membrane fluidity of ECV 304 was measured with the fluorescence polarization method. Results After ECV 304 was cultured in hypoxia and the the lack of glucose for 24 hours, the release of LDH and the membrane fluidity were increased significantly; NO level was decreased. Preincubation of ECV 304 with 20, 80,160 μ mol·L -1 of Quercetin for 24 hours reduced LDH activity, membrane fluidity and increased the level of NO in hypoxia and the lack of glucose induced ECV 304. Conclusion These results demonstrate that Quercetin can produce the protective effect on hypoxia and the lack of glucose induced injury of ECV 304 by increasing release of NO and changing membrane fluidity.展开更多
Chinese President Xi Jinping awarded Cambodian Queen Mother,Norodom Monineath Sihanouk,the Friendship Medal of the People’s Republic of China at a ceremony at the Great Hall of the People in Beijing on November 6.Que...Chinese President Xi Jinping awarded Cambodian Queen Mother,Norodom Monineath Sihanouk,the Friendship Medal of the People’s Republic of China at a ceremony at the Great Hall of the People in Beijing on November 6.Queen Mother Monineath is an important witness and promoter of China-Cambodia friendship,who has forged a tight bond with China since she accompanied Norodom Sihanouk,then Cambodian prince,to China in 1958 for the first time,Xi said when delivering a speech.展开更多
Objective In drugs for i nvigorating blood circulation, to find a herb that can stimulate afferent discha rge of muscle spindle. Methods A single muscle spindle was isolated from sartorial mus cle of toad. Using ai...Objective In drugs for i nvigorating blood circulation, to find a herb that can stimulate afferent discha rge of muscle spindle. Methods A single muscle spindle was isolated from sartorial mus cle of toad. Using air-gap technique, afferent discharge of the muscle spindle was recorded. Effects of Angelica Sinensis, Salvia Miltiorrhiza, and Safflower o n afferent discharge of the muscle spindle were observed. Results Angelica Sinensis could distinctly increase afferent di scharge frequency of the muscle spindle, and this increase was dose-dependent. But Salvia Miltiorrhiza and Safflower had no this excitatory effect. Conclusion It is known that Angelica Sinensis can invigorate bl ood circulation, and we have found its excitatory effect on muscle spindle which makes it possible to serve people with muscle atrophy if more evidences from cl inical experiments are available.展开更多
Introduction Les découvertes numismatiques récentes justifient un réexamen de la période mal connue des dernières années de la dynastie artaxiade en Grande-Arménie(fondée vers 1...Introduction Les découvertes numismatiques récentes justifient un réexamen de la période mal connue des dernières années de la dynastie artaxiade en Grande-Arménie(fondée vers 189 av.J.-C.par Artaxias Ier),et plus particulièrement la période concernant le ou les règnes d’Erato,reine d’Arménie.展开更多
Un célèbre virtuose des arts martiaux,une épée mystérieuse,des combats aériens alternants,l'humanité et la justice,ce sont des éléments culturels qui se déploient ...Un célèbre virtuose des arts martiaux,une épée mystérieuse,des combats aériens alternants,l'humanité et la justice,ce sont des éléments culturels qui se déploient dans Tigre et Dragon,film réalisé en 2000 par Ang Lee.En Occident,où le style 《 de cape et d'épée 》 chinois était moins connu,展开更多
Exploring multi-target drugs(MTDs)is a prevalent method against Alzheimer’s disease(AD).However,the current developed MTDs based on the“framework combination”technique or using flavonoids have not realized better h...Exploring multi-target drugs(MTDs)is a prevalent method against Alzheimer’s disease(AD).However,the current developed MTDs based on the“framework combination”technique or using flavonoids have not realized better healing effects owing to a complex metabolic process and low bioavailability.Here,we propose an alternative strategy to develop self-assembled nanoparticles(NPs)as MTDs using 9-fluorenylmethyloxycarbonyl-tryptophan(Fmoc-Trp),quercetin(Que),and Fe^(2+)as building blocks through coordination and electrostatic interaction to generate Fmoc-Trp-Fe^(2+)-Que(FTFQ)NPs.Whether in cell or in vivo,FTFQ NPs exhibited considerable biocompatibility attributing to the inherent biological affinity of assembly units.By combining the advantages of assemble approach and nanostructures,the obtained FTFQ NPs greatly enhanced the bioavailability of Que and displayed synergistic therapeutic effects through reducing Aβaggregation in direct/indirect means and eliminating the toxicity induced by ROS and Aβoligomer/fibrils.Furthermore,FTFQ NPs could obviously restore the AD zebrafish’s mobility and stimulus response ability.More importantly,developing self-assembly NPs as MTDs could be an efficient and general method to promote other novel MTDs by extending the assemble units to other drugs,peptides,and metal ions.Based on above benefits,these self-assembled NPs could be as potential MTDs and show great promising application in AD treatment.展开更多
文摘Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia and the lack of glucose. Methods The experiments were performed in the culture of ECV 304 injured with hypoxia and the lack of glucose in vitro. The releases of intracellular lactate dehydrogenase(LDH) of ECV 304 was measured with automatic biochemistry analysis. NO level of ECV 304 was monitored with colorimetry. The membrane fluidity of ECV 304 was measured with the fluorescence polarization method. Results After ECV 304 was cultured in hypoxia and the the lack of glucose for 24 hours, the release of LDH and the membrane fluidity were increased significantly; NO level was decreased. Preincubation of ECV 304 with 20, 80,160 μ mol·L -1 of Quercetin for 24 hours reduced LDH activity, membrane fluidity and increased the level of NO in hypoxia and the lack of glucose induced ECV 304. Conclusion These results demonstrate that Quercetin can produce the protective effect on hypoxia and the lack of glucose induced injury of ECV 304 by increasing release of NO and changing membrane fluidity.
文摘Chinese President Xi Jinping awarded Cambodian Queen Mother,Norodom Monineath Sihanouk,the Friendship Medal of the People’s Republic of China at a ceremony at the Great Hall of the People in Beijing on November 6.Queen Mother Monineath is an important witness and promoter of China-Cambodia friendship,who has forged a tight bond with China since she accompanied Norodom Sihanouk,then Cambodian prince,to China in 1958 for the first time,Xi said when delivering a speech.
文摘Objective In drugs for i nvigorating blood circulation, to find a herb that can stimulate afferent discha rge of muscle spindle. Methods A single muscle spindle was isolated from sartorial mus cle of toad. Using air-gap technique, afferent discharge of the muscle spindle was recorded. Effects of Angelica Sinensis, Salvia Miltiorrhiza, and Safflower o n afferent discharge of the muscle spindle were observed. Results Angelica Sinensis could distinctly increase afferent di scharge frequency of the muscle spindle, and this increase was dose-dependent. But Salvia Miltiorrhiza and Safflower had no this excitatory effect. Conclusion It is known that Angelica Sinensis can invigorate bl ood circulation, and we have found its excitatory effect on muscle spindle which makes it possible to serve people with muscle atrophy if more evidences from cl inical experiments are available.
文摘Introduction Les découvertes numismatiques récentes justifient un réexamen de la période mal connue des dernières années de la dynastie artaxiade en Grande-Arménie(fondée vers 189 av.J.-C.par Artaxias Ier),et plus particulièrement la période concernant le ou les règnes d’Erato,reine d’Arménie.
文摘Un célèbre virtuose des arts martiaux,une épée mystérieuse,des combats aériens alternants,l'humanité et la justice,ce sont des éléments culturels qui se déploient dans Tigre et Dragon,film réalisé en 2000 par Ang Lee.En Occident,où le style 《 de cape et d'épée 》 chinois était moins connu,
基金supported by the National Natural Science Foundation(No.22076024)the Natural Science Foundation of Fujian Province(No.2021J01552)Fuzhou University Testing Fund of Precious Apparatus(No.2020T005).
文摘Exploring multi-target drugs(MTDs)is a prevalent method against Alzheimer’s disease(AD).However,the current developed MTDs based on the“framework combination”technique or using flavonoids have not realized better healing effects owing to a complex metabolic process and low bioavailability.Here,we propose an alternative strategy to develop self-assembled nanoparticles(NPs)as MTDs using 9-fluorenylmethyloxycarbonyl-tryptophan(Fmoc-Trp),quercetin(Que),and Fe^(2+)as building blocks through coordination and electrostatic interaction to generate Fmoc-Trp-Fe^(2+)-Que(FTFQ)NPs.Whether in cell or in vivo,FTFQ NPs exhibited considerable biocompatibility attributing to the inherent biological affinity of assembly units.By combining the advantages of assemble approach and nanostructures,the obtained FTFQ NPs greatly enhanced the bioavailability of Que and displayed synergistic therapeutic effects through reducing Aβaggregation in direct/indirect means and eliminating the toxicity induced by ROS and Aβoligomer/fibrils.Furthermore,FTFQ NPs could obviously restore the AD zebrafish’s mobility and stimulus response ability.More importantly,developing self-assembly NPs as MTDs could be an efficient and general method to promote other novel MTDs by extending the assemble units to other drugs,peptides,and metal ions.Based on above benefits,these self-assembled NPs could be as potential MTDs and show great promising application in AD treatment.
