Smoking during pregnancy is a major source of fetal exposure to numerous harmful agents present in tobacco smoke. Lung development involves complex biochemical processes resulting in dramatic changes which continue ev...Smoking during pregnancy is a major source of fetal exposure to numerous harmful agents present in tobacco smoke. Lung development involves complex biochemical processes resulting in dramatic changes which continue even after birth. In addition to type I cells which form the blood-air barrier, type II alveolar epithelial (AE) cells have important and diverse functions related to immunological protection and stabilization of the alveolus through synthesis and secretion of the pulmonary surfactant. Apoptosis or programmed cells death is an important physiological process during lung embryogenesis and for the proper maintenance of homeostasis. Caspases are proteases that play important roles in regulating apoptosis. Caspase-3 is the key executioner caspase in the cascade of events leading to cell death by apoptosis. We explored the hypothesis that cigarette smoke extract (CSE) induces apoptosis in fetal rat lung type II AE cells by activation of caspase-3. To analyze these factors, isolated fetal rat lung type II AE cells were used. The cells were exposed to different concentrations of CSE (5%, 10% or 15%) (v/v) for 60 min. The results of the present study showed that CSE induced apoptosis in fetal rat lung type II AE cells with a significant increase (p 0.05) in caspase-3 activity and decrease in cell proliferation at CSE concentrations of 10% and 15% (v/v). These observations indicate that cigarette smoke extract induces apoptosis by activation of caspase-3 in fetal rat lung type II AE cells in a dose-dependent manner and may potentially alter the regulated development of the lung and the appearance of the surfactant-producing type II alveolar cells which are critical for the establishment of adequate gas exchange at birth.展开更多
Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer.Quercetin,a flavonoid with low toxicity,widely exists in various fruits and vegetables.It has...Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer.Quercetin,a flavonoid with low toxicity,widely exists in various fruits and vegetables.It has the potential to be a therapeutic agent against various cancers.This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2 cells.Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay.Induction of cell apoptosis was confirmed by apoptotic cells population(sub-G1 peak)detected by flow cytometer.A decrease in mitochondrial membrane potential and caspase-3 activation were also demonstrated in this study.Furthermore,quercetin induced HepG2 cell apoptosis through ROS-mediated phosphorylated ataxia-telangiectasia mutated,c-Jun Nterminal kinases,signal transducer,and activator of transcription 3(STAT-3),and Bax signaling pathways.These results suggest that quercetin has the potential to become an effective drug against the tumor.展开更多
The effect of quercetin （Que） on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry...The effect of quercetin （Que） on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry （FCM）, and the caspase-3 expression of each group by colorimetry set respectively. Quercetin inhibited HEN1 cells in in a dose-（r=0.709, P〈0.01） and time-dependent manner （r=0.703, P〈0.01）. The ratio of apoptotic and necrosis cells was increased in the cells treated with quercetin. Cell cycle was specificly arrested in G2/M phase. Apoptosis cusp was revealed by FCM. The activity of caspase-3 was significantly up-regulated in 5 groups treated with quecetin as compared with control group （P〈0.05）. It was concluded that the growth inhibition of quercetin was highly related to cell cycle arrest at the G2/M phase and induction of caspase-dependent apoptosis in human nasopharyngeal carcinoma HEN 1 cells.展开更多
Cancer is a worldwide disease that ranks as the second-largest cause of death after cardiovascular disease. In 2019, the estimated number of cancer deaths was around 10 million worldwide and 600,000 in the United Stat...Cancer is a worldwide disease that ranks as the second-largest cause of death after cardiovascular disease. In 2019, the estimated number of cancer deaths was around 10 million worldwide and 600,000 in the United States. Due to the high side effects of the available treatments for cancer, such as chemotherapy and radiotherapy, a demand for alternative treatments has been a goal for scientists with less toxicity and side effects. Lung cancer is the most common type of cancer worldwide and the leading cause of death in the United States. In addition, prostate cancer is the second-leading cause of cancer death in American men after lung cancer. Natural sources discovered to have therapeutic medicinal properties against human diseases are the plants Peganum harmala and Haloxylon salicornicum. The aim of this study is to test the anticancer activity of leaf extracts of Peganum harmala and Haloxylon salicornicum on lung cancer (A549) and prostate cancer (PC3) cell lines. The anticancer activity of P. harmala and H. salicornicum was studied using the assays: crystal violet viability, lipid peroxidation, and caspase-3 techniques, which were evaluated in vitro on two human cancer cell lines. The results of the study showed that both plants’ extracts reduced the viability of both cancer cell lines, which depended on the concentration of the extract. Also, P. harmala showed more potent activity against both types of cancer than H. salicornicum. P. harmala and H. salicornicum decreased the lipid peroxidation that induces cancer as well as increased the expression of caspase-3, which causes apoptosis and cell death. This study concluded that P. harmala and H. salicornicum leaf extracts showed significant anticancer properties, which might be due to the presence of phytochemicals, including flavonoids and phenolics.展开更多
文摘Smoking during pregnancy is a major source of fetal exposure to numerous harmful agents present in tobacco smoke. Lung development involves complex biochemical processes resulting in dramatic changes which continue even after birth. In addition to type I cells which form the blood-air barrier, type II alveolar epithelial (AE) cells have important and diverse functions related to immunological protection and stabilization of the alveolus through synthesis and secretion of the pulmonary surfactant. Apoptosis or programmed cells death is an important physiological process during lung embryogenesis and for the proper maintenance of homeostasis. Caspases are proteases that play important roles in regulating apoptosis. Caspase-3 is the key executioner caspase in the cascade of events leading to cell death by apoptosis. We explored the hypothesis that cigarette smoke extract (CSE) induces apoptosis in fetal rat lung type II AE cells by activation of caspase-3. To analyze these factors, isolated fetal rat lung type II AE cells were used. The cells were exposed to different concentrations of CSE (5%, 10% or 15%) (v/v) for 60 min. The results of the present study showed that CSE induced apoptosis in fetal rat lung type II AE cells with a significant increase (p 0.05) in caspase-3 activity and decrease in cell proliferation at CSE concentrations of 10% and 15% (v/v). These observations indicate that cigarette smoke extract induces apoptosis by activation of caspase-3 in fetal rat lung type II AE cells in a dose-dependent manner and may potentially alter the regulated development of the lung and the appearance of the surfactant-producing type II alveolar cells which are critical for the establishment of adequate gas exchange at birth.
基金This work was supported by theGuangdong Natural Science Foundation(2020A1515110648)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)+2 种基金the Technology Planning Project of Guangzhou(202102010202 and 202201020655)the Open Project of Guangdong Key Laboratory of Marine Materia Medica(LMM2020-7)the Guangzhou Medical University Students’Science and Technology Innovation Project(02-408-2203-2079,2021AEK119,2021AEK122,2021AEK125 and 2021AEK128).
文摘Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer.Quercetin,a flavonoid with low toxicity,widely exists in various fruits and vegetables.It has the potential to be a therapeutic agent against various cancers.This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2 cells.Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay.Induction of cell apoptosis was confirmed by apoptotic cells population(sub-G1 peak)detected by flow cytometer.A decrease in mitochondrial membrane potential and caspase-3 activation were also demonstrated in this study.Furthermore,quercetin induced HepG2 cell apoptosis through ROS-mediated phosphorylated ataxia-telangiectasia mutated,c-Jun Nterminal kinases,signal transducer,and activator of transcription 3(STAT-3),and Bax signaling pathways.These results suggest that quercetin has the potential to become an effective drug against the tumor.
文摘The effect of quercetin （Que） on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry （FCM）, and the caspase-3 expression of each group by colorimetry set respectively. Quercetin inhibited HEN1 cells in in a dose-（r=0.709, P〈0.01） and time-dependent manner （r=0.703, P〈0.01）. The ratio of apoptotic and necrosis cells was increased in the cells treated with quercetin. Cell cycle was specificly arrested in G2/M phase. Apoptosis cusp was revealed by FCM. The activity of caspase-3 was significantly up-regulated in 5 groups treated with quecetin as compared with control group （P〈0.05）. It was concluded that the growth inhibition of quercetin was highly related to cell cycle arrest at the G2/M phase and induction of caspase-dependent apoptosis in human nasopharyngeal carcinoma HEN 1 cells.
文摘Cancer is a worldwide disease that ranks as the second-largest cause of death after cardiovascular disease. In 2019, the estimated number of cancer deaths was around 10 million worldwide and 600,000 in the United States. Due to the high side effects of the available treatments for cancer, such as chemotherapy and radiotherapy, a demand for alternative treatments has been a goal for scientists with less toxicity and side effects. Lung cancer is the most common type of cancer worldwide and the leading cause of death in the United States. In addition, prostate cancer is the second-leading cause of cancer death in American men after lung cancer. Natural sources discovered to have therapeutic medicinal properties against human diseases are the plants Peganum harmala and Haloxylon salicornicum. The aim of this study is to test the anticancer activity of leaf extracts of Peganum harmala and Haloxylon salicornicum on lung cancer (A549) and prostate cancer (PC3) cell lines. The anticancer activity of P. harmala and H. salicornicum was studied using the assays: crystal violet viability, lipid peroxidation, and caspase-3 techniques, which were evaluated in vitro on two human cancer cell lines. The results of the study showed that both plants’ extracts reduced the viability of both cancer cell lines, which depended on the concentration of the extract. Also, P. harmala showed more potent activity against both types of cancer than H. salicornicum. P. harmala and H. salicornicum decreased the lipid peroxidation that induces cancer as well as increased the expression of caspase-3, which causes apoptosis and cell death. This study concluded that P. harmala and H. salicornicum leaf extracts showed significant anticancer properties, which might be due to the presence of phytochemicals, including flavonoids and phenolics.
