Delonix elata L.is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases,liver diseases and rheumatic problems.However,systematic evaluation of its wound healing activity is lackin...Delonix elata L.is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases,liver diseases and rheumatic problems.However,systematic evaluation of its wound healing activity is lacking.Thus,in the present study,we aimed to assess the wound healing activity of D.elata stem bark extract(DSE) and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside(QRPG) in rats.The formulations effects on wound healing were assessed by the wound contraction rate,epithelialization period,tensile strength,content of the hydroxyproline,hexosamine and uronic acid in granulation tissue,histopathological studies and Col 1 α(I) expression level in wound tissue by reverse transcription polymerase chain reaction(RT-PCR) study.The topical application of DSE ointment caused faster epithelialization,significant wound contraction(100%),and better tensile strength(710.5 ± 10.5 g/cm^2),while QRPG showed wound epithelialization with 98.2%contraction,better than that of the control group(78.18%).The biochemical analysis of granulation tissue revealed that DSE and QRPG significantly increased hydroxyproline,hexosamine and uronic acid content.A significant increase in the expression of Col 1 α(I) was observed in the wound tissue of DSE and QRPG treated rats.DSE and QRPG were shown to enhance wound healing by increasing collagen synthesis through upregulation of Col 1 α(I),thus validating ethnomedicinal uses.展开更多
The core of the natural product chemistry laboratory is isolation secondary metabolit</span><span style="font-family:Verdana;">e</span><span style="font-family:""><s...The core of the natural product chemistry laboratory is isolation secondary metabolit</span><span style="font-family:Verdana;">e</span><span style="font-family:""><span style="font-family:Verdana;">s. One of the potential secondary metabolites for isolation in the natural product chemistry laboratory is routine (quercetin-3-O-glycosides). Routine (Quercetin-3-O-glycoside) has been isolated from ethanol extract of rubber cassava leaves (</span><i><span style="font-family:Verdana;">Manihot glaziovii </span></i><span style="font-family:Verdana;">MA). Isolation was done by maceration and recrystallization. The isolation method used in this study is complemented by the isolation method published. The isolated (Quercetin-3-O-glycoside) routine using this method obtained a yield of 0.118% of the total dried leaf extract. The routine (Quercetin-3-O-glycoside) was identified using a standard routine. Routine can be further utilized in the world of medicine as an amplifier of capillary structure, reducing the permeability and fragility of blood vessels.展开更多
基金partially supported by the Department of Biotechnology,New Delhi,India(Grant no.BT/PR11505/SPD/24/337/2008BT/PR9128/INF/22/190/2013)
文摘Delonix elata L.is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases,liver diseases and rheumatic problems.However,systematic evaluation of its wound healing activity is lacking.Thus,in the present study,we aimed to assess the wound healing activity of D.elata stem bark extract(DSE) and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside(QRPG) in rats.The formulations effects on wound healing were assessed by the wound contraction rate,epithelialization period,tensile strength,content of the hydroxyproline,hexosamine and uronic acid in granulation tissue,histopathological studies and Col 1 α(I) expression level in wound tissue by reverse transcription polymerase chain reaction(RT-PCR) study.The topical application of DSE ointment caused faster epithelialization,significant wound contraction(100%),and better tensile strength(710.5 ± 10.5 g/cm^2),while QRPG showed wound epithelialization with 98.2%contraction,better than that of the control group(78.18%).The biochemical analysis of granulation tissue revealed that DSE and QRPG significantly increased hydroxyproline,hexosamine and uronic acid content.A significant increase in the expression of Col 1 α(I) was observed in the wound tissue of DSE and QRPG treated rats.DSE and QRPG were shown to enhance wound healing by increasing collagen synthesis through upregulation of Col 1 α(I),thus validating ethnomedicinal uses.
文摘The core of the natural product chemistry laboratory is isolation secondary metabolit</span><span style="font-family:Verdana;">e</span><span style="font-family:""><span style="font-family:Verdana;">s. One of the potential secondary metabolites for isolation in the natural product chemistry laboratory is routine (quercetin-3-O-glycosides). Routine (Quercetin-3-O-glycoside) has been isolated from ethanol extract of rubber cassava leaves (</span><i><span style="font-family:Verdana;">Manihot glaziovii </span></i><span style="font-family:Verdana;">MA). Isolation was done by maceration and recrystallization. The isolation method used in this study is complemented by the isolation method published. The isolated (Quercetin-3-O-glycoside) routine using this method obtained a yield of 0.118% of the total dried leaf extract. The routine (Quercetin-3-O-glycoside) was identified using a standard routine. Routine can be further utilized in the world of medicine as an amplifier of capillary structure, reducing the permeability and fragility of blood vessels.
