Four Dimensions of Integrating Chinese Culture Into College English Teaching From the Perspective of Tyler’s Basic Principles

Integrating Chinese culture into college English can not only enhance students’humanities literacy and cultivate their cultural confidence,but also facilitate the inheritance and international dissemination of Chinese culture.Taking Tyler’s curriculum framework as the starting point,this paper analyzes some factors that affect the integration of Chinese culture into the college English teaching and proposes some strategies for the integration of Chinese culture into college English teaching by innovating teaching objectives,enriching teaching contents,transforming modes of course delivery,and reconstructing the assessment system.

Cultures and Subcultures

A cross-cultural analysis of an extract of the book Lost in Translation is provided in the essay. Definitions of culture and subculture are discussed and different theories from Hofstede and Schwartz are referred to in order to validate the analysis.

Simplified three-dimensional culture system for long-term expansion of embryonic stem cells

AIM: To devise a simplified and efficient method for long-term culture and maintenance of embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem cells(ESCs) labeled with enhanced yellow fluorescent protein were cultured in three-dimensional(3-D) self-assembling scaffolds and compared with traditional two-dimentional(2-D) culture techniques requiring mouse embryonic fibroblast feeder layers or leukemia inhibitory factor. 3-D scaffolds encapsulating ESCs were prepared by mixing ESCs with polyethylene glycol tetra-acrylate(PEG-4-Acr) and thiolfunctionalized dextran(Dex-SH). Distribution of ESCs in 3-D was monitored by confocal microscopy. Viability and proliferation of encapsulated cells during long-term culture were determined by propidium iodide as well as direct cell counts and PrestoB lue(PB) assays. Genetic expression of pluripotency markers(Oct4, Nanog, Klf4, and Sox2) in ESCs grown under 2-D and 3-D cultureconditions was examined by quantitative real-time polymerase chain reaction. Protein expression of selected stemness markers was determined by two different methods, immunofluorescence staining(Oct4 and Nanog) and western blot analysis(Oct4, Nanog, and Klf4). Pluripotency of 3-D scaffold grown ESCs was analyzed by in vivo teratoma assay and in vitro differentiation via embryoid bodies into cells of all three germ layers. RESULTS: Self-assembling scaffolds encapsulating ESCs for 3-D culture without the loss of cell viability were prepared by mixing PEG-4-Acr and Dex-SH(1:1 v/v) to a final concentration of 5%(w/v). Scaffold integrity was dependent on the degree of thiol substitution of Dex-SH and cell concentration. Scaffolds prepared using Dex-SH with 7.5% and 33% thiol substitution and incubated in culture medium maintained their integrity for 11 and 13 d without cells and 22 ± 5 d and 37 ± 5 d with cells, respectively. ESCs formed compact colonies, which progressively increased in size over time due to cell proliferation as determined by confocal microscopy and PB staining. 3-D scaffold cultured ESCs expressed significantly higher levels(P < 0.01) of Oct4, Nanog, and Kl4, showing a 2.8, 3.0 and 1.8 fold increase, respectively, in comparison to 2-D grown cells. A similar increase in the protein expression levels of Oct4, Nanog, and Klf4 was observed in 3-D grown ESCs. However, when 3-D cultured ESCs were subsequently passaged in 2-D culture conditions, the level of these pluripotent markers was reduced to normal levels. 3-D grown ESCs produced teratomas and yielded cells of all three germ layers, expressing brachyury(mesoderm), NCAM(ectoderm), and GATA4(endoderm) markers. Furthermore, these cells differentiated into osteogenic, chondrogenic, myogenic, and neural lineages expressing Col1, Col2, Myog, and Nestin, respectively. CONCLUSION: This novel 3-D culture system demonstrated long-term maintenance of mouse ESCs without the routine passaging and manipulation necessary for traditional 2-D cell propagation.

Establishment of the Culture Technique o f Pulmonary Vascular Pericytes and Its Identification in Rats

Summary: In order to study the cellular origin of muscularization in non muscular arterioles of the lung, the pulmonary vascular pericytes culture was established. The terminal lung tissue of the rat was taken out and minced. Then 0.5 % of type Ⅳ collagenase solution was added for digestion and the microvascular segments were obtained by screening. The targeted cells were cultured by “selective conditioned media”. Under phase contrast microscope, the cultured cells were large in size with ragged margin and numerous pseudopodia, which imparted tubule like structure. There was no contact inhibition in growing cells, so multiple layers developed. When they were confluent, there were morphologically no “hillock and dale” growth pattern as in smooth muscle cells or “weave like” pattern as in fibroblasts. The ultrastructure of cultured cells showed numerous digital processes, moderate amount of rough and smooth endoplasmic reticulum, rich Golgi's apparatus, microfilaments, few lysosomes without myofilaments and dense bodies. Immunohistochemical staining revealed that the cultured pericytes had same kind of cellular skeletal protein, α SM actin, like smooth muscle cells. The cultured cells also exhibited positive reaction to CD 34 antigen and S 100 antigen, which were negative in smooth muscle cells and fibroblasts. The cell growth pattern, ultrastructure and immunological phenotype suggested that the cultured cells had characteristics of vascular pericytes. Pericytes are one of the components of microvascular cells, and the establishment of in vitro culture technique of pericytes is of significance for further exploration of the muscularization of non muscular arterioles in lung and the mechanism of structural remodeling of pulmonary vessels.

Feminism, Patriarchy and Culture Dominance ——A review to "The Woman Warrior

The Woman Warrior" is an influential book in the 20th century making a thunder of voice in the world to accuse the stereotype of patriarchy and racism, which also shows the Chinese Americans’ difficulty of being edged to the cultural cliff. This article analyses the content and the style of the novel to see inside what the writer wants to express and to figure out the real reason of the inequality between sexes and reveal the truth of the western culture dominance. Feminism, Patriarchy and culture are the main factors considered in the article.

On Characteristics and Cultures of the Hakka's Enclosed Houses in South Jiangxi Province

General situation and distribution of round houses of the Hakka in south Jiangxi were introduced, such architectural styles as enclosed house with a square yard (kouzi wei, in the shape of 口), enclosed house with a yard of three transverse and one longitudinal constructions (guozi wei, in the shape of 国), and enclosed house with a round house in its yard (tao wei) were taken to analyze features of the Hakka's enclosed houses. It was highlighted that the Hakka's enclosed houses in south Jiangxi has diversified architectural styles and outstanding defensive and living functions. Construction art and religious culture shown by the enclosed houses were interpreted, and the branch of Han people with unique dialects, cultures, folk customs and emotional attitudes was proposed as a miniature of the culture in south Jiangxi. Moreover, it was stressed that more attention of the public and more efforts for the protection should be given to the Hakka's cultures in south Jiangxi, carriers of the locals' history over the past thousands of years.

A Study of Pearl Buck’s Translation View of Cultural Symbiosis

Based on the theory of cultural symbiosis put forward by Mr.Zhang Liwen,this paper summarizes the translation view of cultural symbiosis,and uses it to explain the relevant characteristics of the Noble laureate Pearl Buck’s English translation of All Men Are Brothers.This paper,through laying out Buck’s cultural harmony value full in her translated work,probes into the connotation,manifestation,and enlightenment of Pearl Buck’s idea of a harmonious symbiosis of Chinese and Western cultures.

Rural Tourism, Shanxi Merchants’ Culture and New Rural Construction of Socialism

Rural tourism was a new form of tourism gradually emerging in China in recent years. It satisfied urban residents' pursuit of nature and had a promising market prospect. New rural construction of socialism was an important historical task in the process of economic and social development of China at present and in future period. New rural construction was the important basis of rural tourism development and meanwhile rural tourism development could promote new rural construction. Shanxi Merchant's Culture of Xi Village in Jinzhong City was a kind of excellent rural tourism resource. Developing rural tourism was one of the effective ways to promote new rural construction of socialism in Xi Village.

Cross-Cultural Adjustment and Coping:U.S.Students in Taiwan Residents Higher Education

The aim of this study was to investigate the adjustment problems of students from the United States enrolled in universities in the East,specifically in Taiwan,their problems related to cultural adaptation,and the process of adjustment to student life in Taiwan.Under investigation were cultural adjustment and coping skills as these students transitioned from West to East.Qualitative data were collected from interviews with participants and faculty members as well as participant observations.Results indicated that U.S.students found their own ways to acclimate to their new academic setting as well as to social relations,cross-cultural issues,and the linguistic environment in Taiwan to achieve effective adaptation.They made changes in themselves to cope with all situations they encountered.This study provides suggestions for international students abroad in Taiwan,for the Taiwan Residents government,and for universities or colleges in terms of what they should offer to current and future international students.

The Rise of Aesthetic Anthropology in the Marginal Culture

At the turn of the century, the development of aesthetics encounters severe challenges and is trapped in a crisis. To get out of it, it is necessary to dig deeper in the study of aesthetics. The fact that the marginal areas＇ cultural heritage, agricultural culture, industrial culture and post-industrial culture often coexist in a harmonious way in a nation or the same area attracts the attention of aesthetic anthropologists. The paper mainly analyzes the constructive relationship between the culture of marginal areas and aesthetic anthropology, and discusses how to establish a relationship between them with the help of the development model of Yunnan＇s marginal culture.

Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes

AIM: To study the effects of aminoguanidine (AG) and two L-arginine analogues N(omega)-nitro-L-arginine methyl ester (L-NAME) and N(omega)-nitro-L-arginine (L-NNA) on nitric oxide (NO) production induced by cytokines (TNF-alpha, IL-1 beta, and IFN-gamma) and bacterial lipopolysaccharide (LPS) mixture (CM) in the cultured rat hepatocytes, and examine their mechanisms action. METHODS: Rat hepatocytes were incubated with AG, L-NAME, L-NNA, Actinomycin D (ActD) and dexamethasone in a medium containing CM (LPS plus TNF-alpha, IL-1 beta, and IFN-gamma) for 24h. NO production in the cultured supernatant was measured with the Griess reaction. Intracellular cGMP level was detected with radioimmunoassy. RESULTS: NO production was markedly blocked by AG and L-NAME in a dose-dependent manner under inflammatory stimuli condition triggered by CM in vitro. The rate of the maximum inhibitory effects of L-NAME (38.9%) was less potent than that obtained with AG(53.7%, P 【 0.05). There was no significant difference between the inhibitory effects of AG and two L-arginine analogues on intracellular cGMP accumulation in rat cultured hepatocytes. Non-specific NOS expression inhibitor dexamethasone (DEX)and iNOS mRNA transcriptional inhibitor ActD also significantly inhibited CM-induced NO production. AG(0.1 mmol x L(-1)) and ActD (0.2 ng x L(-1)) were equipotent in decreasing NO production induced by inflammatory stimuli in vitro, and both effects were more potent than that induced by non-selectivity NOS activity inhibitor L-NAME (0.1 mmol x L(-1)) under similar stimuli conditions (P【0.01). CONCLUSION: AG is a potent selective inhibitor of inducible isoform of NOS,and the mechanism of action may be not only competitive inhibition in the substrate level, but also the gene expression level in rat hepatocytes.

The Impact of Employees＇ National/Regional Culture on the Adaptation to Organizational Culture

In respect of all forms of enterprises, whether it is a private or public institution, labor cohesion and productivity is one of the most emphasized issues. Culture, on the other hand, can be characterized as one of the inextricable and hardly ever alterable features of human kind. Whether the profession cohesion and accordingly productivity of those employed within a culture discrepant from their culture of origin both due to the effect of globalization and domestic conditions are culturally affected is a question of importance, which must be answered with regard to state, institution, and employee. The occupational obligation to work in various regions, encountered in public employees at a national scale, particularly in the fields of education and health, and identified with legislative regulations, has become a reason of preference due to economic justifications for private sector employees. Hofstede （1990）, who divides culture, in general, into two, national and organizational, has presented five parameters for the elucidation of cultures. These are ＂power distance＂, ＂individualism vs. collectivism＂, ＂masculinity vs. femininity＂, ＂uncertainty avoidance＂, and ＂long and short term orientation＂. By means of power distance, the conception by an organization or social structure of power distribution of its members is queried. In other words, the difficulty or simplicity in people establishing communication with more influential individuals of the society is evaluated. Individualism vs. collectivism is with regard to whether individuals consider themselves as a distinct individual or a member of a group. Masculinity vs. femininity is the parameter, explanatory of the mentality, rather than a form of gender discrimination. Masculine cultures attach more importance to competition, force, and achievement whereas feminine societies place emphasis on emotions and life quality. Uncertainty avoidance is associated with the attitude of societies towards uncertainties. While some may not pay attention to uncertainties, for others, situations without clear boundaries may be registered as a threat. Long and short term orientation is related to long or short term planned by＇ people and accordingly bearing expectations and in other respects, displays the past, present, and future oriented thinking tendency. Within the scope of this study, it is aimed to identify the positive or negative impact of the culture of origin of university staff, to be designated in Istanbul/Turkey, on the organizational culture. By means of the results of the aforementioned study, the comparison of cohesion is in terms of ability to communicate and accordingly productivity, within the frame of Hofstede＇s cultural dimensions, between those employed within their culture （of origin） and those employed in different cultures. In pursuant of the results obtained from this study, an opinion can be formed for the regulations to be issued on the matter on national scale. At institutional scale, contributions can be made in the assessment of employee productivity from the aforesaid perspective and in terms of the employee, awareness can be raised on the impact of working within the body of different cultures.

Culture and Leadership in the 21 st Century

Some factors that have contributed to the need for organizational leaders to adapt, and adjust to other national cultures are the globalization of the workforce, the expansion of international companies, and exposure of many national organizations to increased business competition. The increased competition has forced organizational leaders to engage in the understanding of the cultural limitations of organizations and their leadership practices. In addition, cross-cultural leadership researchers in 21st century are cognizant of the fact that culture is a condition, and a boundary to previous leadership theories and practices. Using Gerte Hofstede＇s cultural dimension model, culture and organizational leadership as they relate to the leadership practices of participants, and National and Family Cultures are examined in this revised paper.

Culture Street—The Hub of Tianjin's Cultural Roots

Culture Street is the place which no visitor to Tianjin will want to miss. There one can find Qing-style mansions stretching for more than six hundred metres, displays of antiques and per-formances of well-preserved cultural activities. The street is located at the northeastern corner of old Tianjin city where the roots of the city’s history and culture lie. Two hundred years ago when Tianjin was a small port, Tianhou (Goddess of the Temple) was already there. Merchants and those who were traveling away from home by sea would all go to the temple to ask for the god-

On The Northeast Regional Culture in Xiao Hong’s Novels

As the literary pride from the black land of northeast China,Xiao hong’s works show the unique regional culture of northeast China.With her meticulous brush strokes,she showed us the distinct geographical environment of the four seasons in north China,the strong local flavor and people’s sentiment,as well as the servility and ferocity of the people in the northeast.Xiao hong’s criticism of callous and ignorant servile characters and his praise of the consciousness of resistance and the indomitable life form,prompts us to think about the“national character”of northeast China and makes us feel the tragedy of society,the philosophy of life and the spirit of poets.

Differential development and electrophysiological activity in cultured cortical neurons from the mouse and cynomolgus monkey

In vitro cultures of primary cortical neurons are widely used to investigate neuronal function.However,it has yet to be fully investigated whether there are significant differences in development and function between cultured rodent and primate cortical neurons,and whether these differences influence the utilization of cultured cortical neurons to model pathological conditions.Using in vitro culture techniques combined with immunofluorescence and electrophysiological methods,our study found that the development and maturation of primary cerebral cortical neurons from cynomolgus monkeys were slower than those from mice.We used a microelectrode array technique to compare the electrophysiological differences in cortical neurons,and found that primary cortical neurons from the mouse brain began to show electrical activity earlier than those from the cynomolgus monkey.Although cultured monkey cortical neurons developed slowly in vitro,they exhibited typical pathological features-revealed by immunofluorescent staining-when infected with adeno-associated viral vectors expressing mutant huntingtin(HTT),the Huntington’s disease protein.A quantitative analysis of the cultured monkey cortical neurons also confirmed that mutant HTT significantly reduced the length of neurites.Therefore,compared with the primary cortical neurons of mice,cultured monkey cortical neurons have longer developmental and survival times and greater sustained physiological activity,such as electrophysiological activity.Our findings also suggest that primary cynomolgus monkey neurons cultured in vitro can simulate a cell model of human neurodegenerative disease,and may be useful for investigating time-dependent neuronal death as well as treatment via neuronal regeneration.All mouse experiments and protocols were approved by the Animal Care and Use Committee of Jinan University of China(IACUC Approval No.20200512-04)on May 12,2020.All monkey experiments were approved by the IACUC protocol(IACUC Approval No.LDACU 20190820-01)on August 23,2019 for animal management and use.

Kluckhohn and Strodbeck＇s value model in Chinese and American culture

Culture is the holistic interrelationship of a group＇s identity, beliefs, values, activities, rules, customs, communication patterns, and institutions （Dodd, 1998）. Values are important components of a given culture accepted by individuals sharing that culture. They are deep and hidden elements of the cultural iceberg that are seldom questioned or defended but can reflect different characteristics of different cultures. Kluckhohn and Strodbeck＇s value model is used here to analyze distinct Chinese and American culture.

Historical Contributions and Innovative Development of China's Inclusive Culture

Inclusive culture is a dynamic pattern of cultural integration and innovative development formed in the interactions between man and nature, man and society, and man and others. Chinese culture is among the sources of inclusive culture. Being inheritable, open, assimilative, innovative and self–driven, this inclusive culture significantly contributes to China's ideological evolution, ethnic integration, religious development, economic openness, literature and art prosperity, as well as political wisdom in building a community with a shared future for mankind. Inclusiveness is key to the lasting and sustainable development of traditional Chinese culture. During China's construction of a great modern socialist country, the CPC's leadership over cultural innovation and development must be strengthened; a strong cultural confidence must always be maintained; the prosperity of socialist culture with Chinese characteristics must be further enhanced; the sustainable and integrated development of socialist culture with Chinese characteristics must be promoted; people's need for an enriched cultural life must be satisfied; and more efforts must be made to build a community with a shared future for mankind. Only in this way can the light of China's inclusive culture illuminate the future of globalization.

The Influence of Miao Nationality’s Culture in Yuan Xiang Region on the Later Poetry Creation of Qu Yuan

Miao culture is one of the minority cultures in China.It also has a certain impact on the development of Chinese literature and poetry.Chu ci is a new poetic style created by Qu Yuan,an ancient poet in China.It is the first collection of romantic poetry in the history of Chinese literature.Qu Yuan’s poetry creation is related to his life experience.Qu Yuan was exiled to Yuan Xiang region,and he had been in contact with the Miao ancestors in Yuan Xiang area during the exile.Therefore,the Miao land culture in Yuan Xiang area has a profound influence on Qu Yuan’s later poetry creation.This paper,taking Qu Yuan’s later poetry as an example,analyzes the influence of Miao culture on Chu ci in Yuan Xiang area,introduces Qu Yuan’s life and how to contact Miao culture,and probes into the influence of Miao nationality’s religious thought,customs,language and culture,oral folk song and pan song on the later poetry creation of Qu Yuan.