Effect of Quyu Chencuo Formula(去菀陈莝方)on Renal Fibrosis in Obstructive Nephropathy Rats

Objective: To observe the effect of Quyu Chencuo Formula(去菀陈莝方, QCF) on renal fibrosis in rats with obstructive nephropathy. Methods: Twenty-four rats were randomly divided into three groups, 4 for sham operation as the control group, 10 for unilateral ureteral obstruction(UUO) model group, and the rest 10 for QCF treating UUO model group. All rats were sacrificed under 3% pentobarbital(50 mg/kg) anesthesia on the 14 th day after surgery, then the right kidney samples of rats were harvested for hematoxylin eosin(HE) staining and Masson staining to observe the renal pathological changes. Immunohistochemistry and Western blotting were used to examine the expression of transforming growth factor β1(TGF-β1), and real-time polymerase chain reaction(RT-PCR) was employed to examine the expressions of TGF-β1, α-smooth muscle actin(α-SMA) and E-cadherin mRNA. Results: HE and Masson staining showed that the renal interstitial of the rats in the control group had no significant fibrotic lesion; in the model group, there were obvious interstitial fibrosis; for the QCF group, there were epithelial cell necrosis, infiltration of lymphocytes and mononuclear cells, aggravated interstitial fibrosis in varied degrees, but the pathological changes were less in the QCF group than in the model group. The immunohistochemistry and Western blotting results showed that the TGF-β1 expression was increased significantly in the model group, while decreased significantly in the QCF group(P<0.05); RT-PCR showed that the mRNA expression of α-SMA and TGF-β1 increased significantly in the model group, while both were significantly decreased in the QCF group compared with the model group(P<0.05). The mRNA expression of E-cadherin was decreased significantly in the model group, and it was significantly increased in the QCF group as compared with the model group(P<0.05). Conclusion: QCF may improve renal fibrosis by regulating the expressions of TGF-β1, α-SMA and E-cadherin, and prevent the progress of kidney fibrosis.

也说“去宛陈莝”

认为“去宛陈莝”从语言结构上分析,是一个述宾短语,意义是“去莝”,而“宛陈”则是定语成分,以修饰宾语“莝”。原文“去宛陈莝”四字无误,既没有语序上的问题,也没有误、脱、衍、倒之类的抄刻讹谬。其字面义是剔除淤积陈腐的碎草,在论文中取此意象来比喻去除体内的淤积陈腐之物。

万冬桂从“去菀陈莝”治疗乳腺癌相关淋巴水肿经验

万冬桂教授辨析乳腺癌术后上肢淋巴水肿,认为疾病病位在脉络,属本虚标实、因虚致实之证,水饮、瘀血是疾病的关键病理因素,脏腑亏虚是疾病的关键病理基础,水瘀互结,脉络瘀阻是疾病的关键病机,疾病前驱期主要病机为气血亏虚,水瘀初生;症状明显期主要病机为脏腑虚损,水瘀渐盛;转归期主要病机为水瘀胶结,留恋难去。临证应以去菀陈莝为总治则,“去”已成之瘀血、水饮的同时补脏腑亏虚以根“去”酿生“瘀血、水饮”之因。前驱期主要治法为健脾补肾,补气养血;症状明显期主要治法为通补合用,活血利水;转归期主要治法为活血化瘀,攻逐水饮,临床疗效突出。本文对其学术思想及临床经验详细阐述,以期为乳腺癌相关上肢淋巴水肿的治疗拓宽思路。

从“去菀陈莝”谈新型冠状病毒肺炎的治疗

《黄帝内经》中所言“去菀陈莝”,“菀陈”当指郁积体内的水湿、痰饮、瘀血等病理产物。新型冠状病毒肺炎(简称新冠肺炎)以湿、热、痰、瘀、毒、虚为主,病程中出现湿郁肺卫、痰湿阻肺、湿毒弥漫、痰瘀互结,内闭外脱等病理变化。治疗当遵《黄帝内经》“去菀陈莝”之法,分别予化湿通阳、宣肺健脾、肃肺化痰祛湿、疏利三焦气机、祛痰逐瘀利水,开肺气固脱等治疗。贯穿“开鬼门、洁净府”之法,以去除湿、痰、瘀等体内的“菀陈”,达到“平治于权衡”的效果。促使本病的痊愈。