Quzhou Aurantii Fructus(QAF)has a long history as a folk medicine and food for the treatment of liver diseases.While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids a...Quzhou Aurantii Fructus(QAF)has a long history as a folk medicine and food for the treatment of liver diseases.While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF,the potential preventative effects afforded by essential oil components present within QAF remains enigmatic.In this study,we prepared Quzhou Aurantii Fructus essential oil(QAFEO)and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine(LPS/D-GalN)induced acute liver failure(ALF)mouse models.Using RNA-sequence(RNA-seq)analysis,we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways.QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1(TBK1),TGF-beta activated kinase 1(TAK1),interferon regulatory factor 3(IRF3),and the activation of mitogen activated kinase-like protein(MAPK)and nuclear factor-kappa B(NF-κB)pathways in vivo and in vitro.Importantly,QAFEO substantially reduced myeloid differentiation primary response gene 88(MyD88)-toll-like receptor 4(TLR4)interaction levels.Moreover,8 compounds from QAFEO could directly bind to REAL,TAK1,MyD88,TBK1,and IRF3.Taken together,the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response.展开更多
From the perspective of cultural communication,this paper studies the cultural and economic value of the inheritance and promotion of Quzhou food culture,and analyzes the problems of Quzhou food culture in the process...From the perspective of cultural communication,this paper studies the cultural and economic value of the inheritance and promotion of Quzhou food culture,and analyzes the problems of Quzhou food culture in the process of communication due to the narrow market,insufficient publicity,and lack of clear development ideas.By using the current popular new media means to spread the characteristics and flavors of Quzhou food,it is proposed to inherit and promote Quzhou food culture by creating brand effect of Quzhou food enterprises,setting Quzhou food websites,and shooting food documentaries,which will help publicize Quzhou food culture to the outside world,and thus play a positive role in promoting the local economic development of Quzhou.展开更多
With the increasing popularity of new media,new media marketing plays an increasingly important role in the marketing of tourism scenic spots,bringing a new marketing opportunity to tourism scenic spots.Based on the 4...With the increasing popularity of new media,new media marketing plays an increasingly important role in the marketing of tourism scenic spots,bringing a new marketing opportunity to tourism scenic spots.Based on the 4R theory in marketing theory,this paper takes Quzhou Jianglang Mountain as the research object and finds that the following problems exist in the new media marketing of Quzhou Jianglang Mountain:1.The new media users are not closely enough connected;2.the response to the new media market changes is not fast enough;3.the new media marketing relationship interaction is not timely enough;and 4.the return of new media marketing is not ideal.The new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot is proposed from four perspectives:association,reaction,relationship,and return.Finally,the research on the new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot based on the 4R theory is summarized.展开更多
提出了题示方法测定衢枳壳样品中黄曲霉毒素B1、B2、G1、G2和赭曲霉毒素A的含量。样品经过体积比79∶20∶1的乙腈-水-乙酸混合溶液超声提取,离心,上清液用p H 7.0磷酸盐缓冲液稀释,用复合免疫亲和柱净化,过滤,滤液中的目标物在XDB C18...提出了题示方法测定衢枳壳样品中黄曲霉毒素B1、B2、G1、G2和赭曲霉毒素A的含量。样品经过体积比79∶20∶1的乙腈-水-乙酸混合溶液超声提取,离心,上清液用p H 7.0磷酸盐缓冲液稀释,用复合免疫亲和柱净化,过滤,滤液中的目标物在XDB C18色谱柱上用不同体积比的体积比1∶9的甲醇-5 mmol·L^(-1)乙酸铵混合溶液和体积比9∶1的甲醇-5 mmol·L^(-1)乙酸铵混合溶液的混合溶液分离,电喷雾离子源正离子(ESI+)和多反应监测(MRM)模式检测,同位素内标法定量。结果显示:5种真菌毒素与其同位素内标质量浓度比和对应的峰面积比在一定范围内呈线性关系,检出限为0.05~0.20μg·kg^(-1)。3个加标浓度水平下阴性样品中5种真菌毒素的回收率为82.7%~97.3%,测定值的相对标准偏差(n=6)为5.2%~12%。展开更多
基金Zhejiang Provincial Natural Science Foundation of China[LQ22H270014]the Chinese medicine science foundation of Zhejiang Province,China[2020ZA120]+1 种基金Quzhou technology projects,China[2022K53,2023K113,2023K120]Science and technology project of Zhejiang Provincial Drug Administration[2021013].
文摘Quzhou Aurantii Fructus(QAF)has a long history as a folk medicine and food for the treatment of liver diseases.While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF,the potential preventative effects afforded by essential oil components present within QAF remains enigmatic.In this study,we prepared Quzhou Aurantii Fructus essential oil(QAFEO)and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine(LPS/D-GalN)induced acute liver failure(ALF)mouse models.Using RNA-sequence(RNA-seq)analysis,we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways.QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1(TBK1),TGF-beta activated kinase 1(TAK1),interferon regulatory factor 3(IRF3),and the activation of mitogen activated kinase-like protein(MAPK)and nuclear factor-kappa B(NF-κB)pathways in vivo and in vitro.Importantly,QAFEO substantially reduced myeloid differentiation primary response gene 88(MyD88)-toll-like receptor 4(TLR4)interaction levels.Moreover,8 compounds from QAFEO could directly bind to REAL,TAK1,MyD88,TBK1,and IRF3.Taken together,the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response.
基金one of the results of“Research and Translation of Quzhou Traditional Food Culture and Stories”(Project No.:2021R435014)supported by University Students’Scientific and Technological Innovation Program in Zhejiang Province。
文摘From the perspective of cultural communication,this paper studies the cultural and economic value of the inheritance and promotion of Quzhou food culture,and analyzes the problems of Quzhou food culture in the process of communication due to the narrow market,insufficient publicity,and lack of clear development ideas.By using the current popular new media means to spread the characteristics and flavors of Quzhou food,it is proposed to inherit and promote Quzhou food culture by creating brand effect of Quzhou food enterprises,setting Quzhou food websites,and shooting food documentaries,which will help publicize Quzhou food culture to the outside world,and thus play a positive role in promoting the local economic development of Quzhou.
基金sponsored by the following projects:Multi-modal Discourse Analysis of the Quzhou Government Affairs New Media(Grant No.202111488039).
文摘With the increasing popularity of new media,new media marketing plays an increasingly important role in the marketing of tourism scenic spots,bringing a new marketing opportunity to tourism scenic spots.Based on the 4R theory in marketing theory,this paper takes Quzhou Jianglang Mountain as the research object and finds that the following problems exist in the new media marketing of Quzhou Jianglang Mountain:1.The new media users are not closely enough connected;2.the response to the new media market changes is not fast enough;3.the new media marketing relationship interaction is not timely enough;and 4.the return of new media marketing is not ideal.The new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot is proposed from four perspectives:association,reaction,relationship,and return.Finally,the research on the new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot based on the 4R theory is summarized.
