Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared wit...Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable pathogen.Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding.In this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages.Using genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,respectively.Compared with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits.Therefore,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.展开更多
推动制造业高质量发展是构建新发展格局的重中之重,是实现质量强国战略的关键之举。为了解制造业企业质量管理相关研究现状与热点分布,以CNKI与Web of Science数据库中的中外文献为研究对象,运用CiteSpace 6.2.R6软件对文献进行计量分析...推动制造业高质量发展是构建新发展格局的重中之重,是实现质量强国战略的关键之举。为了解制造业企业质量管理相关研究现状与热点分布,以CNKI与Web of Science数据库中的中外文献为研究对象,运用CiteSpace 6.2.R6软件对文献进行计量分析,总结其研究脉络与演进趋势发现,国外对制造业企业质量管理的研究更加广泛与深入,质量成本管理是国内该领域研究的一大特色,下一阶段国内外研究的重点都集中在智能制造与工业4.0上。国内外研究趋势基本相同,我国对制造业企业质量成本的研究不存在明显滞后性,下一阶段应加强对研究广度与深度的探索,为制造业企业质量管理研究提供参考。展开更多
为解析大豆R6期籽粒氨基酸含量的遗传机制,本研究利用264份大豆种质资源材料在2020年和2021年测定了与菜用大豆食味品质相关的精氨酸、丙氨酸、谷氨酸和天冬氨酸含量,并进行全基因组关联分析(GWAS)。结果表明,2年共检测到89个与大豆R6...为解析大豆R6期籽粒氨基酸含量的遗传机制,本研究利用264份大豆种质资源材料在2020年和2021年测定了与菜用大豆食味品质相关的精氨酸、丙氨酸、谷氨酸和天冬氨酸含量,并进行全基因组关联分析(GWAS)。结果表明,2年共检测到89个与大豆R6期籽粒4种氨基酸含量显著关联的SNP位点,其中有5个标记能同时被2年或2个性状重复检测到,分别为S03_40647948(Chr.3)、S05_2727464(Chr.5)、S10_4122977(Chr.10)、S17_34559022(Chr.17)和S19_48541685(Chr.19),单个位点可以解释11.25%~28.19%的表型变异解,其中Chr.17上的标记S17_34559022在2020年和2021年被共同检测到与谷氨酸含量显著关联,属稳定遗传的位点。共挖掘出9个候选基因,其中ZIP蛋白(zinc finger family protein)、转录因子bHLH(bHLH DNA-binding superfamily protein)、生长素反应蛋白家族(auxin-responsive protein family)和天冬氨酸蛋白酶家族蛋白(aspartyl protease family protein),可能是影响菜用大豆氨基酸代谢的重要基因。本研究挖掘到的5个氨基酸含量主效SNP位点和9个候选基因,有助于解析大豆R6期籽粒氨基酸含量的遗传基础及其调控机制,为菜用大豆食味品质遗传改良奠定了基础。展开更多
基金This research was supported by the National Key Research and Development Program of China(2021YFD1200600)the National Natural Science Foundation of China(32272105).
文摘Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable pathogen.Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding.In this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages.Using genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,respectively.Compared with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits.Therefore,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.
文摘推动制造业高质量发展是构建新发展格局的重中之重,是实现质量强国战略的关键之举。为了解制造业企业质量管理相关研究现状与热点分布,以CNKI与Web of Science数据库中的中外文献为研究对象,运用CiteSpace 6.2.R6软件对文献进行计量分析,总结其研究脉络与演进趋势发现,国外对制造业企业质量管理的研究更加广泛与深入,质量成本管理是国内该领域研究的一大特色,下一阶段国内外研究的重点都集中在智能制造与工业4.0上。国内外研究趋势基本相同,我国对制造业企业质量成本的研究不存在明显滞后性,下一阶段应加强对研究广度与深度的探索,为制造业企业质量管理研究提供参考。
文摘为解析大豆R6期籽粒氨基酸含量的遗传机制,本研究利用264份大豆种质资源材料在2020年和2021年测定了与菜用大豆食味品质相关的精氨酸、丙氨酸、谷氨酸和天冬氨酸含量,并进行全基因组关联分析(GWAS)。结果表明,2年共检测到89个与大豆R6期籽粒4种氨基酸含量显著关联的SNP位点,其中有5个标记能同时被2年或2个性状重复检测到,分别为S03_40647948(Chr.3)、S05_2727464(Chr.5)、S10_4122977(Chr.10)、S17_34559022(Chr.17)和S19_48541685(Chr.19),单个位点可以解释11.25%~28.19%的表型变异解,其中Chr.17上的标记S17_34559022在2020年和2021年被共同检测到与谷氨酸含量显著关联,属稳定遗传的位点。共挖掘出9个候选基因,其中ZIP蛋白(zinc finger family protein)、转录因子bHLH(bHLH DNA-binding superfamily protein)、生长素反应蛋白家族(auxin-responsive protein family)和天冬氨酸蛋白酶家族蛋白(aspartyl protease family protein),可能是影响菜用大豆氨基酸代谢的重要基因。本研究挖掘到的5个氨基酸含量主效SNP位点和9个候选基因,有助于解析大豆R6期籽粒氨基酸含量的遗传基础及其调控机制,为菜用大豆食味品质遗传改良奠定了基础。
