MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer

AIM: To elucidate the potential biological role of mi R-30 b in gastric cancer and investigate the underlying molecular mechanisms of mi R-30 b to inhibit metastasis of gastric cancer cells.METHODS: The expression of mi R-30 b was detected in gastric cancer cell lines and samples by reverse transcription-polymerase chain reaction. CCK-8 assays were conducted to explore the impact of mi R-30 b overexpression on the proliferation of gastric cancer cells. Flow cytometry was used to examine the effect of mi R-30 b on the apoptosis. Transwell test was used for the migration and invasion assays. Luciferase reporter assays and Western blot were employed to validate regulation of putative target of mi R-30 b.RESULTS: The results showed that mi R-30 b was downregulated in gastric cancer tissues and cancer cell lines and functioned as a tumor suppressor. Overexpression of mi R-30 b promoted cell apoptosis,and suppressed proliferation,migration and invasion of the gastric cancer cell lines AGS and MGC803. Bioinformatic analysis identified the 3'-untranslated region of eukaryotic translation initiation factor 5A2(EIF5A2) as a putative binding site of mi R-30 b. Luciferase reporter assays and Western blot analysis confirmed the EIF5A2 gene as a target of mi R-30 b. Moreover,expression levels of theEIF5A2 targets E-cadherin and Vimentin were altered following transfection of mi R-30 b mimics.CONCLUSION: Our findings describe a link between mi R-30 b and EIF5A2,which plays an important role in mediating epithelial-mesenchymal transition.

hsa-miR-30b靶基因预测及其相关生物信息学分析

目的通过生物信息学分析预测hsa-mi R-30b靶基因和功能,为深入研究hsa-mi R-30b的生物学功能和调控机制提供理论指导。方法利用Pub Med检索mi R-30b相关文章,通过mi RBase在线工具分析mi R-30b序列和基因组特征。应用mi RWalk综合数据库对hsa-mi R-30b靶基因进行预测,对靶基因集合应用Cytoscape及其插件Bingo进行功能富集分析,应用DAVID数据库进行靶基因信号转导通路富集分析。mi RBase、Pub Med、mi RWalk数据库通过输入hsa-mi R-30b或mi R-30b名称进行检索,Cytoscape及其插件Bingo和DAVID数据库则输入hsa-mi R-30b靶基因进行分析。结果 mi R-30b序列在多物种间具有一定的保守性。mi RWalk综合数据库预测靶基因交集共125个。hsa-mi R-30b靶基因主要富集于生长发育、细胞迁移、细胞周期、胰腺细胞分化、转录调控、神经系统发育等(P<0.01);KEGG生物通路主要富集于Notch信号通路及年轻起病成人型糖尿病、致心律失常性右心室心肌病、小细胞肺癌和前列腺癌疾病通路(P<0.05)。结论 hsa-mi R-30b预测的靶基因集合富集于多个生物学过程及疾病通路,与肺癌、前列腺癌等多种肿瘤密切相关,为hsa-mi R-30b在肿瘤方向的后续研究奠定基础。