AIM: To determine the expression of mi R-422 a in colorectal cancer(CRC) tissues and to further explore the prognostic value and function of mi R-422 a in CRC carcinogenesis.METHODS: mi R-422 a expression was analyzed...AIM: To determine the expression of mi R-422 a in colorectal cancer(CRC) tissues and to further explore the prognostic value and function of mi R-422 a in CRC carcinogenesis.METHODS: mi R-422 a expression was analyzed in 102 CRC tissues and paired normal mucosa adjacent to carcinoma by quantitative real-time PCR. The relationship of mi R-422 a expression with clinicopathological parameters was also analyzed. Kaplan-Meier analysis and Cox multivariate analysis were performed to estimate the potential role of mi R-422 a. Cell proliferation, migration, and invasion were used for in vitro functional analysis of mi R-422 a.RESULTS: The levels of mi R-422 a were dramatically reduced in CRC tissues compared with normal mucosa(P < 0.05), and significantly correlated with local invasion(P = 0.004) and lymph node metastasis(P < 0.001). Kaplan-Meier survival and Cox regression multivariate analyses revealed that mi R-422 a expression(HR = 0.568, P = 0.015) and clinical TNM stage(HR = 2.942, P = 0.003) were independent prognostic factorsfor overall survival in CRC patients. Furthermore, in vitro experiments showed that overexpression of mi R-422 a inhibited the proliferation, migration, and invasion of SW480 and HT-29 cells.CONCLUSION: Down-regulation of mi R-422 a may serve as an independent prognosis factor in CRC. Mi R-422 a functions as a tumor suppressor and regulates progression of CRC.展开更多
基金Supported by the National Natural Science Foundation of China,No.81472025Outstanding Young Scientist Research Award Fund of Shandong Province,No.BS2014YY023+3 种基金Projects of Medical and Health Technology Development Program of Shandong Province,No.2014WS0124Science Foundation of Qilu Hospital of Shandong University,No.2015QLQN37Fundamental Research of Shandong Universitythe National Key Clinical Medical Specialties Foundation
文摘AIM: To determine the expression of mi R-422 a in colorectal cancer(CRC) tissues and to further explore the prognostic value and function of mi R-422 a in CRC carcinogenesis.METHODS: mi R-422 a expression was analyzed in 102 CRC tissues and paired normal mucosa adjacent to carcinoma by quantitative real-time PCR. The relationship of mi R-422 a expression with clinicopathological parameters was also analyzed. Kaplan-Meier analysis and Cox multivariate analysis were performed to estimate the potential role of mi R-422 a. Cell proliferation, migration, and invasion were used for in vitro functional analysis of mi R-422 a.RESULTS: The levels of mi R-422 a were dramatically reduced in CRC tissues compared with normal mucosa(P < 0.05), and significantly correlated with local invasion(P = 0.004) and lymph node metastasis(P < 0.001). Kaplan-Meier survival and Cox regression multivariate analyses revealed that mi R-422 a expression(HR = 0.568, P = 0.015) and clinical TNM stage(HR = 2.942, P = 0.003) were independent prognostic factorsfor overall survival in CRC patients. Furthermore, in vitro experiments showed that overexpression of mi R-422 a inhibited the proliferation, migration, and invasion of SW480 and HT-29 cells.CONCLUSION: Down-regulation of mi R-422 a may serve as an independent prognosis factor in CRC. Mi R-422 a functions as a tumor suppressor and regulates progression of CRC.
