大樱桃新品系R43特性分析及日光温室提早栽培技术

山东东伊食品生产开发有限公司于2017年从上海交通大学引进大樱桃新品系(代号R-43),该品种早熟、口味甜中带酸、口感脆,相较于传统大樱桃口感更适合年轻消费群体。采用吉塞拉6号作砧木,矮化效果明显、早产性好、丰产性稳定,4年生树单株结果可达15~20kg。价格相比现有大樱桃品种平均高出5~6元/kg,增收效果明显。利用日光温室进行提早栽培,在4月上旬达到果实成熟,售价可达200元/kg。

Dephosphorylation of cardiomyocyte Cx43 is associated with myocardial ischemia and reperfusion injury

Objective：Myocardial ischemia/reperfusion（I/R） injury is the leading cause of death in the world. However, the details of the mechanism of its pathophysiology are still unknown. The present study was designed to investigate the role of connexin 43（Cx43） in acute models of myocardial I/R injury. Methods： Male C57BL/6 mice were subjected to myocardial ischemia（45 min） followed by reperfusion（4 hrs） in vivo. The whole operation was monitored using a two-lead ECG. Hearts were harvested and the level of protein was assessed by western blot analysis. Haematoxylin and Eosin（HE） staining was used to detect the extent of neutrophil infiltration. The expression level of IL-6 was detected by ELISA. Results： A murine myocardial I/R injury model was constructed successfully. Phosphorylated Cx43 decreased 83. 45% while non-phosphorylated Cx43 increased 1.62- fold in the myocardium after I/R injury. Neutrophil infiltration and the expression of the inflammatory cytokine IL-6 increased in the myocardium following I/R. Conclusion： During myocardial I/R injury, cardiomyocyte Cx43 is dephosphorylated, and this may be associated with an inflammatory response.

航空航天推进系统用铌基复合材料

介绍了 用于航 空航天推 进系统 的四种不 同铌基复 合材料 ，并对其显 微组织 、机械性能 等进行了综述。

壮通饮预处理对冠心病血瘀证大鼠AngⅡ,AT-1R,Cx43及其对再灌注损伤的影响

目的：观察壮通饮（Zhuangtongyin,ZTY）对心肌缺血再灌注大鼠心肌细胞的血管紧张素Ⅱ（AngⅡ）,血管紧张素Ⅱ1型受体（AT-1R）,缝隙连接蛋白43（Cx43）mRNA表达水平和蛋白水平的影响,并探讨ZTY预处理在大鼠心肌缺血再灌注损伤过程中对大鼠心肌细胞的保护机制。方法：采用随机分组的方法将SPF级SD大鼠分为空白组,假手术组（只穿线,不结扎）,模型组,壮通饮低、中、高剂量组（6.8,13.6,27.2 g·kg^-1）和复方丹参组（0.08 g·kg^-1）。连续灌药4周后,通过结扎左冠状动脉前降支30 min,再灌注60 min,建立冠心病血瘀证再灌注损伤模型,术中监测大鼠心电指标,记录心律失常发生情况,再灌注结束后,取下心脏组织,用实时荧光定量PCR（Real-time PCR）检测大鼠心肌组织中AngⅡ,AT-1R,Cx43 mRNA的表达水平,用蛋白免疫印迹法（Western blot）检测大鼠心肌组织中AT-1R,Cx43的蛋白表达水平。结果：壮通饮各剂量组和模型组比较均可以减少心肌缺血再灌注损伤模型大鼠心律失常的发生（P〈0.05）。与空白组、假手术组比较,模型组、壮通饮各剂量组、复方丹参组的AngⅡ,AT-1R mRNA表达均上调,Cx43 mRNA表达均下降（P〈0.05）。与模型组比较,壮通饮各剂量组、复方丹参组的AngⅡ,AT-1R mRNA表达均下降,Cx43 mRNA表达均上升,各剂量组之间,中剂量组AngⅡ,AT-1R mRNA下降,Cx43 mRNA表达上升最明显（P〈0.05）。壮通饮各剂量组与复方丹参组比较无统计学意义。空白组与假手术组比较,无显著性差异。与空白组、假手术比较,模型组、壮通饮各剂量组、复方丹参组的AT-1R蛋白表达均上调,Cx43蛋白表达均下降（P〈0.05）。与模型组比较,壮通饮各剂量组、复方丹参组的AT-1R蛋白表达均下降,Cx43蛋白表达均上升,各剂量组之间,中剂量组AT-1R蛋白下降,Cx43蛋白表达上升最明显（P〈0.05）。壮通饮各剂量组与复方丹参组对比无统计学意义。结论：ZTY对心肌缺血再灌注损伤有保护作用,降低再灌注心律失常的发生,其作用机制可能与壮通饮能调控AngⅡ-（AT-1R）-Cx43通路,导致Cx43表达上调有关。