应用脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)及随机放大多态性DNA分析(random amplified polymorphicDNA analysis,RAPD)方法对2000年-2004年在杭州地区分离的03:K6型和O4:K8型致病性副溶血弧菌菌株进行分子分型,...应用脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)及随机放大多态性DNA分析(random amplified polymorphicDNA analysis,RAPD)方法对2000年-2004年在杭州地区分离的03:K6型和O4:K8型致病性副溶血弧菌菌株进行分子分型,探讨近年杭州地区分离的O3:K6型和O4:K8型副溶血弧菌菌株与国际“大流行”O3:K6型菌株间的关系.展开更多
The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two t...The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.展开更多
To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamysfarreri population. Thirt...To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamysfarreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-pp, ACP, AK, PK, AAT, SOD, EST) in three butter systems (TC, pH6.9; TMME, pH 7.4; and EBT, pHS.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep.m-l, Pep.pp. Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.展开更多
Ten populations of the whitefly Bemisia tabaci (Gennadius) collected from eight fieed and ornamental crops from seven provinces/cities in China were determined using RAPD PCR. Results showed that the 10 populati...Ten populations of the whitefly Bemisia tabaci (Gennadius) collected from eight fieed and ornamental crops from seven provinces/cities in China were determined using RAPD PCR. Results showed that the 10 populations of B.tabaci could be classified into three distinct groups: a Biotype B group, a Guangxi group, and a Fujian group. The whiteflies in the Biotype B group were collected from sweetpotato and cotton in Beijing, tomato in Shandong, poinsettia in Shanghai and Guangdong, cabbage in Fujian, and hemp and eggplant in Xinjiang. The Guangxi group were collected from pumpkin in Nanning, Guangxi, and the Fujian group, from sweetpotato in Fujian. It was notable that B.tabaci specimens collected on cabbage in Fujian belonged to the Biotype B group. It is clear that Biotype B does now occur in China, in Beijing, Shandong, Xinjiang, Shanghai, Fujian and Guangdong. It is likely that the Guangxi and Fujian groups are native to China.展开更多
文摘应用脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)及随机放大多态性DNA分析(random amplified polymorphicDNA analysis,RAPD)方法对2000年-2004年在杭州地区分离的03:K6型和O4:K8型致病性副溶血弧菌菌株进行分子分型,探讨近年杭州地区分离的O3:K6型和O4:K8型副溶血弧菌菌株与国际“大流行”O3:K6型菌株间的关系.
基金Supported by National Natural Science Foundation of China (No. 30571445)National High-Technology Research and Development Program (863 Program, No. 2006AA10A404)Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017)
文摘The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.
基金Supported by Chinese Basic Research Project (G1999012007) and Na-tional Natural Science Foundation of China (No. 39700017).
文摘To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamysfarreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-pp, ACP, AK, PK, AAT, SOD, EST) in three butter systems (TC, pH6.9; TMME, pH 7.4; and EBT, pHS.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep.m-l, Pep.pp. Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.
文摘Ten populations of the whitefly Bemisia tabaci (Gennadius) collected from eight fieed and ornamental crops from seven provinces/cities in China were determined using RAPD PCR. Results showed that the 10 populations of B.tabaci could be classified into three distinct groups: a Biotype B group, a Guangxi group, and a Fujian group. The whiteflies in the Biotype B group were collected from sweetpotato and cotton in Beijing, tomato in Shandong, poinsettia in Shanghai and Guangdong, cabbage in Fujian, and hemp and eggplant in Xinjiang. The Guangxi group were collected from pumpkin in Nanning, Guangxi, and the Fujian group, from sweetpotato in Fujian. It was notable that B.tabaci specimens collected on cabbage in Fujian belonged to the Biotype B group. It is clear that Biotype B does now occur in China, in Beijing, Shandong, Xinjiang, Shanghai, Fujian and Guangdong. It is likely that the Guangxi and Fujian groups are native to China.