The serotonin-immenoreactive neurons in the medullary raphe nuclei that project to thecerebellar cortex were investigated by means of a combined microsphere retrograde transport withimmunofluorescence technique.Subseq...The serotonin-immenoreactive neurons in the medullary raphe nuclei that project to thecerebellar cortex were investigated by means of a combined microsphere retrograde transport withimmunofluorescence technique.Subsequent to microsphere injections into the cerebellar vermis(lobules Ⅴ￣Ⅷ)the cells thus retrogradely labeled were found in all medullary raphe nuclei.78.08% ofthe microsphere-labeled cells were located in the nucleus raphe obscurus.Microsphere-labeled cellswere prominent in the P10 and P11 section levels.Serotonin-immunoreaective neurons were present primarily in the raphe nuclei and adjacent reticular formation.Cells double-labeled by microspheresand serotouin-immunostatning were rare, although there were neurons positive for microsphere orserotonin-immunoreaction alone.The results imply that the medullary raphe nuclei do not seem to bethe origin of serotonin fibers of the cerebellar vermal cortex(lobulcs Ⅴ￣Ⅷ).展开更多
文摘The serotonin-immenoreactive neurons in the medullary raphe nuclei that project to thecerebellar cortex were investigated by means of a combined microsphere retrograde transport withimmunofluorescence technique.Subsequent to microsphere injections into the cerebellar vermis(lobules Ⅴ￣Ⅷ)the cells thus retrogradely labeled were found in all medullary raphe nuclei.78.08% ofthe microsphere-labeled cells were located in the nucleus raphe obscurus.Microsphere-labeled cellswere prominent in the P10 and P11 section levels.Serotonin-immunoreaective neurons were present primarily in the raphe nuclei and adjacent reticular formation.Cells double-labeled by microspheresand serotouin-immunostatning were rare, although there were neurons positive for microsphere orserotonin-immunoreaction alone.The results imply that the medullary raphe nuclei do not seem to bethe origin of serotonin fibers of the cerebellar vermal cortex(lobulcs Ⅴ￣Ⅷ).
