The relationship between Ala/Ser polymorphism in 133 codon of exon 3 region of the RASSF1 gene and genetic susceptibility of lung cancer in Hubei province Han population was investigated by a case-control study. Polym...The relationship between Ala/Ser polymorphism in 133 codon of exon 3 region of the RASSF1 gene and genetic susceptibility of lung cancer in Hubei province Han population was investigated by a case-control study. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique was adopted to analyze the polymorphism of codon 133 of exon 3 in the RASSF1 gene of 100 pathologically diagnosed lung cancer patients, and 100 healthy controls. The relationship between different genotypes and the susceptibility of lung cancer was analyzed. Among 200 blood samples from Han people in Hubei Province, including 100 from lung cancer patients and 100 from healthy controls, the frequencies of Ala/Ala, Ala/Ser, Ser/Ser genotype of the RASSF1 in lung cancer patients were 83%, 16%, 1%, and those in healthy controls was 93%, 7%, 0% respectively, with the difference being statistically significant between two groups （P〈0.05）. The individuals with Ala/Ser genotype had higher risk of suffering from lung cancer, with an OR of 2.341, and 95% CI of 1.009-6.393 respectively. It was concluded that RASSF1Ala133Ser was a susceptible genetic factor of lung cancer. Ala/Ser genotype increased the risk of lung cancer.展开更多
To investigate the relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene, RASSF1A protein expression was measured by Western blotting in 10 specimens of normal bladder ti...To investigate the relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene, RASSF1A protein expression was measured by Western blotting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carcinoma （BTCC）. The promoter methylation in BTCC and normal bladder tissues was detected by methylation-specific PCR （MSP）. The results showed that the expression level of RASSF1A protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not correlated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF 1A protein. It is concluded that RASSF1A gene promoter methylation may contribute to the low level or loss of RASSF1A protein expression, the inactivation of RASSF1A gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.展开更多
Objective： To explore the relationship between microsatellite alterations of RASSFIA gene and the development of cervical carcinoma, and its relationship with HPV16 infection. Methods： Two sites of microsatellite po...Objective： To explore the relationship between microsatellite alterations of RASSFIA gene and the development of cervical carcinoma, and its relationship with HPV16 infection. Methods： Two sites of microsatellite polymorphism of RASSFIA gene were selected. Polymerase chain reaction （PCR） technique was used to detect LOH and MSI in 50 cases of cervical carcinoma and 40 cases of cervical intraepithelial neoplasia （CIN）, and to detect the infection state of HPV16. Results： At D3S1478 and D3S4604, the LOH rates of cervical carcinomas were 32.6% （14/43） and 48.9% （23/47）, the MSI rates were 14% （6/43） and 19.1% （9/47）, respectively. The LOH rates of CINs were 31.4% （11/35） and 39.5% （15/38）, the MSI rates were 11.4% （4/35） and 15.8% （6/38）, respectively. There were no significant differences between cervical carcinomas and CINs in respect to their positive rates of LOH and MSI at D3S1478 and D3S4604 （P〉0.05）. There were significant differences in LOH rates at D3S1478 and D3S4604 between the stage Ⅰ-Ⅱ and Ⅲ-Ⅳ cervical carcinomas and between the well/moderately differentiated cervical carcinomas and the poorly differentiated cervical carcinomas （P〈0.05）. The positive rates of LOH and MSI for CIN Ⅲ and noninvasive cervical carcinomas were higher than those in CIN Ⅰ-Ⅱ. The rates of infection of HPV16 in cervical cancer was obviously higher than that in CIN and in normal cervical tissues （P〈0.05）, and the incidence of LOH of RASSFIA gene was higher in HPV16（＋） than that in HPV16（-） （P〈0.05）. Conclusion： The RASSFIA gene change is a relatively late event in cervical carcinomas. The detection of LOH and MSI of RASSFIA gene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma.展开更多
背景与目的:探讨RAS相关结构家族基因1(RAS association domain family gene 1,RASSF1)在胃腺癌组织细胞中的表达及其临床意义。材料与方法:用逆转录聚合酶链式反应(RT-PCR)方法检测胃癌BGC-823细胞系、50例胃癌组织及相应癌旁正常组织...背景与目的:探讨RAS相关结构家族基因1(RAS association domain family gene 1,RASSF1)在胃腺癌组织细胞中的表达及其临床意义。材料与方法:用逆转录聚合酶链式反应(RT-PCR)方法检测胃癌BGC-823细胞系、50例胃癌组织及相应癌旁正常组织中RASSF1A、RASSF1B和RASSF1C mRNA的表达,以及30例胃癌高危人群、可疑胃癌病人胃黏膜活检标本中的RASSF1基因表达情况;用PCR法检测上述50例胃癌组织中的幽门螺杆菌(Hp)感染率。结果:RASSF1A在胃癌组织中的转录表达缺失率为52%(26/50),其表达缺失率与胃癌分化程度、淋巴结转移及TNM分期相关(P<0.05),但与其组织学类型无相关性。RASSF1A在胃癌高危人群及可疑胃癌病人胃黏膜活检标本中的表达缺失率为20%(6/30),胃癌组织中Hp感染率为64%(32/50),RASSF1A的表达缺失与Hp感染组之间无相关性(P>0.05)。RASSF1B在胃癌组织中的转录表达缺失率为22%(11/50),RASSF1C则全部表达,RASSF1B和RASSF1C在胃癌高危人群及可疑胃癌病人胃黏膜活检标本中全部表达,并且两者与胃癌的组织学类型、分化程度、TNM分期均无相关性。结论:RASSF1A在胃癌组织中存在较高的表达缺失,RASSF1A的表达与胃癌的临床分期和病理分级具有相关性,RASSF1A在胃癌高危人群及可疑胃癌患者胃黏膜活检标本中存在表达缺失,因此有望作为胃癌早期检测的标志物,RASSF1A的表达缺失与Hp感染之间无相关性,它们可能是两个独立的致病因素。展开更多
文摘The relationship between Ala/Ser polymorphism in 133 codon of exon 3 region of the RASSF1 gene and genetic susceptibility of lung cancer in Hubei province Han population was investigated by a case-control study. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique was adopted to analyze the polymorphism of codon 133 of exon 3 in the RASSF1 gene of 100 pathologically diagnosed lung cancer patients, and 100 healthy controls. The relationship between different genotypes and the susceptibility of lung cancer was analyzed. Among 200 blood samples from Han people in Hubei Province, including 100 from lung cancer patients and 100 from healthy controls, the frequencies of Ala/Ala, Ala/Ser, Ser/Ser genotype of the RASSF1 in lung cancer patients were 83%, 16%, 1%, and those in healthy controls was 93%, 7%, 0% respectively, with the difference being statistically significant between two groups （P〈0.05）. The individuals with Ala/Ser genotype had higher risk of suffering from lung cancer, with an OR of 2.341, and 95% CI of 1.009-6.393 respectively. It was concluded that RASSF1Ala133Ser was a susceptible genetic factor of lung cancer. Ala/Ser genotype increased the risk of lung cancer.
基金a grant from the National Natural Sciences Foundation of China (No. 30571858)
文摘To investigate the relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene, RASSF1A protein expression was measured by Western blotting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carcinoma （BTCC）. The promoter methylation in BTCC and normal bladder tissues was detected by methylation-specific PCR （MSP）. The results showed that the expression level of RASSF1A protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not correlated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF 1A protein. It is concluded that RASSF1A gene promoter methylation may contribute to the low level or loss of RASSF1A protein expression, the inactivation of RASSF1A gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.
文摘Objective： To explore the relationship between microsatellite alterations of RASSFIA gene and the development of cervical carcinoma, and its relationship with HPV16 infection. Methods： Two sites of microsatellite polymorphism of RASSFIA gene were selected. Polymerase chain reaction （PCR） technique was used to detect LOH and MSI in 50 cases of cervical carcinoma and 40 cases of cervical intraepithelial neoplasia （CIN）, and to detect the infection state of HPV16. Results： At D3S1478 and D3S4604, the LOH rates of cervical carcinomas were 32.6% （14/43） and 48.9% （23/47）, the MSI rates were 14% （6/43） and 19.1% （9/47）, respectively. The LOH rates of CINs were 31.4% （11/35） and 39.5% （15/38）, the MSI rates were 11.4% （4/35） and 15.8% （6/38）, respectively. There were no significant differences between cervical carcinomas and CINs in respect to their positive rates of LOH and MSI at D3S1478 and D3S4604 （P〉0.05）. There were significant differences in LOH rates at D3S1478 and D3S4604 between the stage Ⅰ-Ⅱ and Ⅲ-Ⅳ cervical carcinomas and between the well/moderately differentiated cervical carcinomas and the poorly differentiated cervical carcinomas （P〈0.05）. The positive rates of LOH and MSI for CIN Ⅲ and noninvasive cervical carcinomas were higher than those in CIN Ⅰ-Ⅱ. The rates of infection of HPV16 in cervical cancer was obviously higher than that in CIN and in normal cervical tissues （P〈0.05）, and the incidence of LOH of RASSFIA gene was higher in HPV16（＋） than that in HPV16（-） （P〈0.05）. Conclusion： The RASSFIA gene change is a relatively late event in cervical carcinomas. The detection of LOH and MSI of RASSFIA gene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma.
文摘背景与目的:探讨RAS相关结构家族基因1(RAS association domain family gene 1,RASSF1)在胃腺癌组织细胞中的表达及其临床意义。材料与方法:用逆转录聚合酶链式反应(RT-PCR)方法检测胃癌BGC-823细胞系、50例胃癌组织及相应癌旁正常组织中RASSF1A、RASSF1B和RASSF1C mRNA的表达,以及30例胃癌高危人群、可疑胃癌病人胃黏膜活检标本中的RASSF1基因表达情况;用PCR法检测上述50例胃癌组织中的幽门螺杆菌(Hp)感染率。结果:RASSF1A在胃癌组织中的转录表达缺失率为52%(26/50),其表达缺失率与胃癌分化程度、淋巴结转移及TNM分期相关(P<0.05),但与其组织学类型无相关性。RASSF1A在胃癌高危人群及可疑胃癌病人胃黏膜活检标本中的表达缺失率为20%(6/30),胃癌组织中Hp感染率为64%(32/50),RASSF1A的表达缺失与Hp感染组之间无相关性(P>0.05)。RASSF1B在胃癌组织中的转录表达缺失率为22%(11/50),RASSF1C则全部表达,RASSF1B和RASSF1C在胃癌高危人群及可疑胃癌病人胃黏膜活检标本中全部表达,并且两者与胃癌的组织学类型、分化程度、TNM分期均无相关性。结论:RASSF1A在胃癌组织中存在较高的表达缺失,RASSF1A的表达与胃癌的临床分期和病理分级具有相关性,RASSF1A在胃癌高危人群及可疑胃癌患者胃黏膜活检标本中存在表达缺失,因此有望作为胃癌早期检测的标志物,RASSF1A的表达缺失与Hp感染之间无相关性,它们可能是两个独立的致病因素。
