目的：探讨CyclinA2基因5′-U T R区rs769236位点多态性与结直肠癌易感性的关系。方法采用病例-对照研究，收集150例结直肠癌患者及150例健康对照者，以聚合酶链反应-限制性片段长度多态性法（PCR-RFLP）检测CyclinA2基因rs769236位点...目的：探讨CyclinA2基因5′-U T R区rs769236位点多态性与结直肠癌易感性的关系。方法采用病例-对照研究，收集150例结直肠癌患者及150例健康对照者，以聚合酶链反应-限制性片段长度多态性法（PCR-RFLP）检测CyclinA2基因rs769236位点单核苷酸多态性。rs769236多态性与结直肠癌发病风险的关系采用非条件logistic回归分析。结果与 rs769236 GG野生基因型相比，rs769236突变基因型（GA＋AA）携带者罹患结直肠癌的风险显著增高（校正 OR＝1．92，95％ CI＝1．10～3．36）。结论 CyclinA2基因rs769236多态性与CRC发病风险增高显著有关，突变基因型（GA＋AA）是人群罹患结直肠癌的独立危险因素。展开更多
为研究青蛤(Cyclina sinensis)对重金属Cu^(2+)的蓄积作用及免疫机能的影响,开展了Cu^(2+)对青蛤的急性毒性实验,观察青蛤在96 h Cu^(2+)半致死浓度和安全浓度胁迫下,不同组织的蓄积趋势,及血淋巴液中SOD、CAT和ACP活性的变化。结果显示...为研究青蛤(Cyclina sinensis)对重金属Cu^(2+)的蓄积作用及免疫机能的影响,开展了Cu^(2+)对青蛤的急性毒性实验,观察青蛤在96 h Cu^(2+)半致死浓度和安全浓度胁迫下,不同组织的蓄积趋势,及血淋巴液中SOD、CAT和ACP活性的变化。结果显示:Cu^(2+)的半致死浓度为0.807 mg/L,安全浓度为0.00807 mg/L。鳃和内脏团组织中蓄积的Cu^(2+)浓度与处理时间呈正相关;在半致死和安全浓度胁迫下,鳃组织中Cu^(2+)蓄积速度均快于内脏团;在半致死浓度胁迫下血淋巴液中SOD、CAT和ACP活性呈现先诱导再抑制的趋势,安全浓度下无明显变化,但均高于对照组水平。展开更多
A series of novel 7 -azaindirubin(1a-g) and 7-azaindirubin(2a,2c,2e and 2f) derivatives were designed and synthesized.Their structures were characterized by ~1H NMR and MS spectroscopy as well as by elemental analysis...A series of novel 7 -azaindirubin(1a-g) and 7-azaindirubin(2a,2c,2e and 2f) derivatives were designed and synthesized.Their structures were characterized by ~1H NMR and MS spectroscopy as well as by elemental analysis.Their inhibitory properties against CDK2/cylinA were evaluated in vitro.In contrast to indirubin,some of the described azaindirubins emerged as potent inhibitors of CDK2/cylinA and compound 2b had more potent activity.Biological tests also showed that nitrogen atom at 7-position of azaindirubi...展开更多
采用静态急性毒性实验研究了重金属Pb^(2+)对青蛤(Cyclina sinensis)的生物急性毒性效应,测定了在96 h Pb^(2+)半致死浓度的1/10(TC组)和1/100(SC组)两个浓度胁迫下,血淋巴液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和溶菌酶(LSZ)活性的变...采用静态急性毒性实验研究了重金属Pb^(2+)对青蛤(Cyclina sinensis)的生物急性毒性效应,测定了在96 h Pb^(2+)半致死浓度的1/10(TC组)和1/100(SC组)两个浓度胁迫下,血淋巴液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和溶菌酶(LSZ)活性的变化。结果显示:Pb^(2+)的96 h LC50为7.938 mg/L;SC组ACP活性表现为诱导-抑制趋势,除4 d外均与对照组差异显著(P<0.05), TC组为抑制趋势,为3个组中的最低,与对照组差异显著(P<0.05);实验组SC组和TC组的AKP、LSZ活性均表现为前期为诱导中后期受抑制的趋势,与对照组差异显著(P<0.05),且TC组活性始终低于SC组,表现出Pb^(2+)的胁迫浓度越高酶活性受到的抑制作用越大。以上结果表明,重金属Pb^(2+)对青蛤的毒性级别为高毒级,能造成青蛤免疫相关酶的活性受到抑制,影响青蛤的免疫能力,而且这种抑制作用随着环境中Pb^(2+)浓度增加而增加。展开更多
文摘A series of novel 7 -azaindirubin(1a-g) and 7-azaindirubin(2a,2c,2e and 2f) derivatives were designed and synthesized.Their structures were characterized by ~1H NMR and MS spectroscopy as well as by elemental analysis.Their inhibitory properties against CDK2/cylinA were evaluated in vitro.In contrast to indirubin,some of the described azaindirubins emerged as potent inhibitors of CDK2/cylinA and compound 2b had more potent activity.Biological tests also showed that nitrogen atom at 7-position of azaindirubi...
文摘采用静态急性毒性实验研究了重金属Pb^(2+)对青蛤(Cyclina sinensis)的生物急性毒性效应,测定了在96 h Pb^(2+)半致死浓度的1/10(TC组)和1/100(SC组)两个浓度胁迫下,血淋巴液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和溶菌酶(LSZ)活性的变化。结果显示:Pb^(2+)的96 h LC50为7.938 mg/L;SC组ACP活性表现为诱导-抑制趋势,除4 d外均与对照组差异显著(P<0.05), TC组为抑制趋势,为3个组中的最低,与对照组差异显著(P<0.05);实验组SC组和TC组的AKP、LSZ活性均表现为前期为诱导中后期受抑制的趋势,与对照组差异显著(P<0.05),且TC组活性始终低于SC组,表现出Pb^(2+)的胁迫浓度越高酶活性受到的抑制作用越大。以上结果表明,重金属Pb^(2+)对青蛤的毒性级别为高毒级,能造成青蛤免疫相关酶的活性受到抑制,影响青蛤的免疫能力,而且这种抑制作用随着环境中Pb^(2+)浓度增加而增加。
