将连续变化的碰撞能量与串联质谱相结合,建立了能量分辨质谱(Energy-resolved mass spectrometry,ERMS)分析方法用以区分和检测人参皂苷同分异构体20(S)-Rf和Rg_(1).ERMS将子离子与母离子强度的比值定义为强度分数(Intensity fraction,I...将连续变化的碰撞能量与串联质谱相结合,建立了能量分辨质谱(Energy-resolved mass spectrometry,ERMS)分析方法用以区分和检测人参皂苷同分异构体20(S)-Rf和Rg_(1).ERMS将子离子与母离子强度的比值定义为强度分数(Intensity fraction,IF),两个子离子强度的比值定义为强度比(Intensity ratio,IR),并通过IF和IR分别对连续变化的碰撞能量作图建立特征碎片离子的IF和IR曲线.虽然20(S)-Rf和Rg_(1)的串联质谱图无明显差异,但是多元统计分析结果显示其特征碎片离子[M-Glc-H]-,[M-2Glc-H]-和[M-2Glc-C_(6)H_(12)-H]-的IF和IR曲线差异显著,有明显的区分边界,可以直接区分20(S)-Rf和Rg_(1).进一步利用ERMS实时分析了原人参三醇型皂苷生物转化产物中20(S)-Rf和Rg_(1)的相对摩尔含量.结果表明,ERMS不需液相色谱分离即可实现20(S)-Rf和Rg_(1)的快速区分和相对含量检测.展开更多
Glioblastoma is one of the most common primary brain tumors, and local recurrence and distant metastasis are common posttreatment manifestations in patients. The overall five-year survival still remains poor. Nuclear ...Glioblastoma is one of the most common primary brain tumors, and local recurrence and distant metastasis are common posttreatment manifestations in patients. The overall five-year survival still remains poor. Nuclear factor erythroid2-related factor2 (Nrf-2) and heme oxygenase-1 (HO-1) have been considered to play major roles in the pathogenesis of many tumors. In this study, the expressions of Nrf-2 and HO-1 in glioblastoma were investigated to explore the possible impacts of them on the growth of glioblastoma. 49 cases of glioblastoma patients were analyzed to summarize their gender, age, tumor recurrence, size of tumor, postoperation radiotherapy and chemotherapy. Immunohistochemistry (SP) was applied to evaluate the expression of Nrf-2 and HO-l in pathological sections of 49 cases of giloblastoma and 23 cases of adjacent control tissues. Results showed that the positive rates of Nrf-2 and HO-1 in the 49 glioblastoma tissue sections were 85.7% and 89.8%, respectively, and that of Nrf-2 and HO-1 were 34.8% and 26.1%, respectively in 23 cases of adjacent control sections. There were significant differences between the two groups (P 0.05). Nrf-2 and HO-1 may play an important role in the pathogenesis of glioblastoma, and might be a potential therapeutic target for glioblastoma.展开更多
文摘将连续变化的碰撞能量与串联质谱相结合,建立了能量分辨质谱(Energy-resolved mass spectrometry,ERMS)分析方法用以区分和检测人参皂苷同分异构体20(S)-Rf和Rg_(1).ERMS将子离子与母离子强度的比值定义为强度分数(Intensity fraction,IF),两个子离子强度的比值定义为强度比(Intensity ratio,IR),并通过IF和IR分别对连续变化的碰撞能量作图建立特征碎片离子的IF和IR曲线.虽然20(S)-Rf和Rg_(1)的串联质谱图无明显差异,但是多元统计分析结果显示其特征碎片离子[M-Glc-H]-,[M-2Glc-H]-和[M-2Glc-C_(6)H_(12)-H]-的IF和IR曲线差异显著,有明显的区分边界,可以直接区分20(S)-Rf和Rg_(1).进一步利用ERMS实时分析了原人参三醇型皂苷生物转化产物中20(S)-Rf和Rg_(1)的相对摩尔含量.结果表明,ERMS不需液相色谱分离即可实现20(S)-Rf和Rg_(1)的快速区分和相对含量检测.
文摘Glioblastoma is one of the most common primary brain tumors, and local recurrence and distant metastasis are common posttreatment manifestations in patients. The overall five-year survival still remains poor. Nuclear factor erythroid2-related factor2 (Nrf-2) and heme oxygenase-1 (HO-1) have been considered to play major roles in the pathogenesis of many tumors. In this study, the expressions of Nrf-2 and HO-1 in glioblastoma were investigated to explore the possible impacts of them on the growth of glioblastoma. 49 cases of glioblastoma patients were analyzed to summarize their gender, age, tumor recurrence, size of tumor, postoperation radiotherapy and chemotherapy. Immunohistochemistry (SP) was applied to evaluate the expression of Nrf-2 and HO-l in pathological sections of 49 cases of giloblastoma and 23 cases of adjacent control tissues. Results showed that the positive rates of Nrf-2 and HO-1 in the 49 glioblastoma tissue sections were 85.7% and 89.8%, respectively, and that of Nrf-2 and HO-1 were 34.8% and 26.1%, respectively in 23 cases of adjacent control sections. There were significant differences between the two groups (P 0.05). Nrf-2 and HO-1 may play an important role in the pathogenesis of glioblastoma, and might be a potential therapeutic target for glioblastoma.
