We report the results of cloning,expression,subcellular localization analysis,and molecular properties of the Epstein-Barr virus （EBV） BFRF3 gene,using several bioinformatics tools.Bioinformatics analysis indicated ...We report the results of cloning,expression,subcellular localization analysis,and molecular properties of the Epstein-Barr virus （EBV） BFRF3 gene,using several bioinformatics tools.Bioinformatics analysis indicated that the encoding protein of EBV BFRF3 gene （designated BFRF3） has a conserved Herpes_capsid domain,which was found to be closely related to the gammaherpesvirus capsid protein family,and is highly conserved among its counterparts.展开更多
RF3 <i>Brassica napus</i> L. (InVigor<sup>TM</sup> Canola) has been in commercial cultivation <span>since 1997. RF3 <i>B. napus</i> was produced by <i>Agrobacterium</...RF3 <i>Brassica napus</i> L. (InVigor<sup>TM</sup> Canola) has been in commercial cultivation <span>since 1997. RF3 <i>B. napus</i> was produced by <i>Agrobacterium</i>-mediated tran</span>sformation to provide glufosniate ammonium herbicide tolerance to the crop. Canola quality (CQ) varieties of <i>Brassica juncea</i> L. (Czern & Coss), a close relative of <i>B. napus</i>, have been available in Canada for more than 20 years. Currently, BASF Agricultural Solutions is developing CQ RF3 <i>B. juncea</i> via conventional breeding with RF3<i> B. napus </i>to provide similar herbicide tolerance in the <i>B.</i> <i>juncea</i> species. The insertion event RF3<i> </i>contains the <i>bar</i> gene <span>(origin <i>Streptomyces hygroscopicus</i>) coding for phosphinothricin acetyl trans</span>ferase (PAT/<i>bar</i>) protein which confers tolerance to glufosinate-ammonium<span> </span>herbicides. RF3 also contains the <i>barstar</i> gene (origin <i>Bacillus amyloliquefa<span>ciens</span></i><span>), coding for the Barstar protein, which is an inhibitor of the Barna</span>se protein. In the absence of Barnase, there is no impact of Barstar. Many safety <span>studies have been conducted during the development of the canola quality lines of RF3 </span><i><span>B. napus</span></i><span> and RF3 </span><i><span>B. juncea</span></i><span>. This report seeks to compare th</span>e <span>two species based on the scientific data generated during the safety asses</span>sments of RF3 <i>B. napus</i> and RF3 <i>B. juncea</i> and discusses the utility of using studies with one species to support the safety of the other species both containing the same insertion event.展开更多
基金supported by grants from Science andTechnology New Star in Zhu Jiang,Guangzhou City(2013J2200018)the Natural Science Foundation ofGuangdong Province(S2013040016596)+5 种基金the National Natural Science Foundation of China(31200120)the Medical Scientific Research Foundation of Guangdong Province,China(B2012165)the Foundation for Distinguished Young Talents in Higher Education of Guangdong,China First Batch of Young Core Instructor of Guangzhou Medical Universitythe Science and Technology Found ationfor Universities in Guangzhou,China(10A173 and08A097)the Students’ Extracurricular ScientificAnd Technological Activities in Guangzhou Medical University (2012A039 and 2012C007)We thank Dr.Teru Kanda for the generous gift of Akata-derivedEBV-BAC
文摘We report the results of cloning,expression,subcellular localization analysis,and molecular properties of the Epstein-Barr virus （EBV） BFRF3 gene,using several bioinformatics tools.Bioinformatics analysis indicated that the encoding protein of EBV BFRF3 gene （designated BFRF3） has a conserved Herpes_capsid domain,which was found to be closely related to the gammaherpesvirus capsid protein family,and is highly conserved among its counterparts.
文摘RF3 <i>Brassica napus</i> L. (InVigor<sup>TM</sup> Canola) has been in commercial cultivation <span>since 1997. RF3 <i>B. napus</i> was produced by <i>Agrobacterium</i>-mediated tran</span>sformation to provide glufosniate ammonium herbicide tolerance to the crop. Canola quality (CQ) varieties of <i>Brassica juncea</i> L. (Czern & Coss), a close relative of <i>B. napus</i>, have been available in Canada for more than 20 years. Currently, BASF Agricultural Solutions is developing CQ RF3 <i>B. juncea</i> via conventional breeding with RF3<i> B. napus </i>to provide similar herbicide tolerance in the <i>B.</i> <i>juncea</i> species. The insertion event RF3<i> </i>contains the <i>bar</i> gene <span>(origin <i>Streptomyces hygroscopicus</i>) coding for phosphinothricin acetyl trans</span>ferase (PAT/<i>bar</i>) protein which confers tolerance to glufosinate-ammonium<span> </span>herbicides. RF3 also contains the <i>barstar</i> gene (origin <i>Bacillus amyloliquefa<span>ciens</span></i><span>), coding for the Barstar protein, which is an inhibitor of the Barna</span>se protein. In the absence of Barnase, there is no impact of Barstar. Many safety <span>studies have been conducted during the development of the canola quality lines of RF3 </span><i><span>B. napus</span></i><span> and RF3 </span><i><span>B. juncea</span></i><span>. This report seeks to compare th</span>e <span>two species based on the scientific data generated during the safety asses</span>sments of RF3 <i>B. napus</i> and RF3 <i>B. juncea</i> and discusses the utility of using studies with one species to support the safety of the other species both containing the same insertion event.
