目的探讨RAD51抑制剂RI-1对MSH2缺陷结直肠癌细胞系的杀伤作用及可能的作用机制。方法 Western blot法筛选MSH2差异表达的结直肠癌细胞系,MTT法检测不同结直肠癌细胞系对RI-1(10、20、30、40或50μmol/L)的敏感性;构建针对MSH2基因的重...目的探讨RAD51抑制剂RI-1对MSH2缺陷结直肠癌细胞系的杀伤作用及可能的作用机制。方法 Western blot法筛选MSH2差异表达的结直肠癌细胞系,MTT法检测不同结直肠癌细胞系对RI-1(10、20、30、40或50μmol/L)的敏感性;构建针对MSH2基因的重组shRNA慢病毒表达载体并感染HT29细胞。RI-1(40μmol/L)处理细胞,流式细胞术检测细胞凋亡的变化;单细胞凝胶电泳实验分析细胞内DNA损伤情况;细胞免疫荧光法检测γ-H2AX foci的形成。结果与对照组相比MSH2缺陷的HCT8细胞有明显细胞凋亡现象(P<0.01);HCT8细胞和HT29 Shmsh2细胞尾部DNA含量、尾长和尾距均较对照组明显增加(P<0.05);HCT8细胞和HT29 Shmsh2细胞内γ-H2AX foci的形成数量较对照组明显增加(P<0.01)。结论 RAD51抑制剂RI-1可能通过增加细胞内DNA损伤参与RI-1对MSH2缺陷肿瘤的杀伤作用。展开更多
Imminent abortion needs more serious attention since it remains a big problem in Indonesia considering its epidemiology, morbidity, mortality,?and prognosis. In fact, some cases can still be prevented.?The objective o...Imminent abortion needs more serious attention since it remains a big problem in Indonesia considering its epidemiology, morbidity, mortality,?and prognosis. In fact, some cases can still be prevented.?The objective of this study was to determine pathogenesis, diagnosis,?and prognosis of imminent abortion through the role of antioxidant vitamin C in the interaction of superoxide dismutase (SOD), interferon-γ?(IFN-γ), interleukin-4 (IL-4), vascular cells adhesion molecule-1?(VCAM-1), and decidual spiral artery resistance index (DSA RI).?This study took 10 months from March to December 2007 at Obstetrics and Gynecology Department of Medical Faculty of?Padjadjaran?University/Hasan Sadikin Hospital, Bandung. Sixty pregnant women who met the inclusion and exclusion criteria were divided into two groups with the random clinical trial method, double-blind with?repeated measurements.?Data were analyzed statistically using t test, Mann-Whitney, Rank?Spearman, Wilcoxon, t paired, and diagnostic test.?The results of this study showed that the incidence of abortion in vitamin C group was 9 cases (30%) and placebo group was 13 cases (43.3%). There was a significant difference in SOD, IFN-γ, IL-4 and VCAM-1 level between groups of women with and without abortion (p < 0.001).?There was a significant difference of SOD level between women with abortion 655.8 (163.6) U/gHb and placebo group 824.5 (106.7) U/gHb after vitamin C administration (p = 0.008). The cutoff point of SOD?was?￡992 U/gHbwhich?showed quite high sensitivity, specificity, accuracy,?and significant difference (p < 0.001). The cutoff point abortion of DSA resistance index was ≥0.55 with 45% accuracy (p = 0.002). The highest change of IL-4 level was 60.7% in vitamin C group and VCAM-1 was?-2.2% (p < 0.05) after?vitamin C administration. There was a negative correlation in SOD changes toward IL-4 changes after vitamin C administration (r =?-0.523) (p = 0.003), a positive correlation between SOD changes and IL-4 changes (0.597) (p < 0.001), a negative correlation between SOD changes and VCAM-1 changes (r =?-0.737) (p < 0.001) and a negative correlation between SOD changes and DSA RI changes (r =?-0.208) (p = 0.022). There were significant changes in the increment of IL-4 and SOD level (p < 0.001) and a decline in IFN-γ, VCAM-1 and DSA RI (p < 0.001) before and after vitamin C administration. In conclusion, there?was a correlation between the?increase of SOD level with?the?decrease of INF-γ?level and?the?increase of IL-4 level in imminent abortion after vitamin C administration. There?was a correlation between?the?increase of SOD level with?the?decrease of DSA RI and?the?decrease of VCAM-1 level in imminent abortion after vitamin C administration. In abortion, there?was?a?decrease of SOD and IL-4 level,?as well as an?increase of IFN-γ, VCAM-1, and DSA RI level.展开更多
The course and severity of periodontitis can be significantly affected by bacterial virulence as well as host immunity dysfunction. Periodontal tissue destruction has been proved to result from cascade of cytokines sy...The course and severity of periodontitis can be significantly affected by bacterial virulence as well as host immunity dysfunction. Periodontal tissue destruction has been proved to result from cascade of cytokines synthesized by reactive cells upon stimulation by pathogenic bacteria and lipopolysaccharides within their cell membranes. The clinical use of genetically programmed cells, producing substances blocking IL-1, based on recombinant IL-1 antagonist, as well as cytokines activating fibroblasts and osteoblasts to regenerate the destroyed periodontal tissue could prove alternative to the conventional treatment. Another cytokine of interest in respect to periodontitis ethiopathogenesis is soluble tumor necrosis factor receptor I (sTNF RI). Observation of soluble TNF receptors as physiologic inhibitors of TNF led to its administration in therapeutic process as well as in therapy selected cases of aggressive periodontitis.展开更多
The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated...The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated through Genefishing analysis during defense responses to Ralstonia solanacearum.The cDNA of RPS 30 contained a 189 base pair(bp)open-reading frame encoding 62 amino acids.The genomic DNA consists of 272 bp containing two exons and one 83 bp intron.The RPS 30 mRNA transcript was mainly expressed in roots and leaves.The expression level of the RPS 30 mRNA transcripts was up-regulated sharply 6 h after bacterial challenge and was 12 times greater than that of the control group.The phylogenetic analysis for genes encoding proteins showed that RPS30 were conserved within dicotyledonous and monocotyledonous plants.d S extremely exceeded d N in all branches of the tree(d N/d S<1.0),indicating that functional constraint have acted on RPS 30 throughout evolution.展开更多
文摘Imminent abortion needs more serious attention since it remains a big problem in Indonesia considering its epidemiology, morbidity, mortality,?and prognosis. In fact, some cases can still be prevented.?The objective of this study was to determine pathogenesis, diagnosis,?and prognosis of imminent abortion through the role of antioxidant vitamin C in the interaction of superoxide dismutase (SOD), interferon-γ?(IFN-γ), interleukin-4 (IL-4), vascular cells adhesion molecule-1?(VCAM-1), and decidual spiral artery resistance index (DSA RI).?This study took 10 months from March to December 2007 at Obstetrics and Gynecology Department of Medical Faculty of?Padjadjaran?University/Hasan Sadikin Hospital, Bandung. Sixty pregnant women who met the inclusion and exclusion criteria were divided into two groups with the random clinical trial method, double-blind with?repeated measurements.?Data were analyzed statistically using t test, Mann-Whitney, Rank?Spearman, Wilcoxon, t paired, and diagnostic test.?The results of this study showed that the incidence of abortion in vitamin C group was 9 cases (30%) and placebo group was 13 cases (43.3%). There was a significant difference in SOD, IFN-γ, IL-4 and VCAM-1 level between groups of women with and without abortion (p < 0.001).?There was a significant difference of SOD level between women with abortion 655.8 (163.6) U/gHb and placebo group 824.5 (106.7) U/gHb after vitamin C administration (p = 0.008). The cutoff point of SOD?was?￡992 U/gHbwhich?showed quite high sensitivity, specificity, accuracy,?and significant difference (p < 0.001). The cutoff point abortion of DSA resistance index was ≥0.55 with 45% accuracy (p = 0.002). The highest change of IL-4 level was 60.7% in vitamin C group and VCAM-1 was?-2.2% (p < 0.05) after?vitamin C administration. There was a negative correlation in SOD changes toward IL-4 changes after vitamin C administration (r =?-0.523) (p = 0.003), a positive correlation between SOD changes and IL-4 changes (0.597) (p < 0.001), a negative correlation between SOD changes and VCAM-1 changes (r =?-0.737) (p < 0.001) and a negative correlation between SOD changes and DSA RI changes (r =?-0.208) (p = 0.022). There were significant changes in the increment of IL-4 and SOD level (p < 0.001) and a decline in IFN-γ, VCAM-1 and DSA RI (p < 0.001) before and after vitamin C administration. In conclusion, there?was a correlation between the?increase of SOD level with?the?decrease of INF-γ?level and?the?increase of IL-4 level in imminent abortion after vitamin C administration. There?was a correlation between?the?increase of SOD level with?the?decrease of DSA RI and?the?decrease of VCAM-1 level in imminent abortion after vitamin C administration. In abortion, there?was?a?decrease of SOD and IL-4 level,?as well as an?increase of IFN-γ, VCAM-1, and DSA RI level.
文摘The course and severity of periodontitis can be significantly affected by bacterial virulence as well as host immunity dysfunction. Periodontal tissue destruction has been proved to result from cascade of cytokines synthesized by reactive cells upon stimulation by pathogenic bacteria and lipopolysaccharides within their cell membranes. The clinical use of genetically programmed cells, producing substances blocking IL-1, based on recombinant IL-1 antagonist, as well as cytokines activating fibroblasts and osteoblasts to regenerate the destroyed periodontal tissue could prove alternative to the conventional treatment. Another cytokine of interest in respect to periodontitis ethiopathogenesis is soluble tumor necrosis factor receptor I (sTNF RI). Observation of soluble TNF receptors as physiologic inhibitors of TNF led to its administration in therapeutic process as well as in therapy selected cases of aggressive periodontitis.
基金Natural Science Funds for Young Scholar of Shandong Academy of Agricultural Sciences,China(No.2015YQN13)Natural Science Foundation of Shandong Province,China(ZR2015YL064)+2 种基金Qingdao Science and Technology Plan Basic Research Project,China(No.12-1-4-11-(1)-jch)China Agricultural Research System(No.CARS-13)Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences,China(No.CXGC2018E21)
文摘The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated through Genefishing analysis during defense responses to Ralstonia solanacearum.The cDNA of RPS 30 contained a 189 base pair(bp)open-reading frame encoding 62 amino acids.The genomic DNA consists of 272 bp containing two exons and one 83 bp intron.The RPS 30 mRNA transcript was mainly expressed in roots and leaves.The expression level of the RPS 30 mRNA transcripts was up-regulated sharply 6 h after bacterial challenge and was 12 times greater than that of the control group.The phylogenetic analysis for genes encoding proteins showed that RPS30 were conserved within dicotyledonous and monocotyledonous plants.d S extremely exceeded d N in all branches of the tree(d N/d S<1.0),indicating that functional constraint have acted on RPS 30 throughout evolution.
