根据美国非营利的教育和文化交流机构国际教育协会(Institute of International Education,IIE)2003年11月公布的年度报告,2002—2003学年,在美外国留学生总数增幅不到1%,但是,来自印度、韩国和肯尼亚的学生人数有显著增加。这份...根据美国非营利的教育和文化交流机构国际教育协会(Institute of International Education,IIE)2003年11月公布的年度报告,2002—2003学年,在美外国留学生总数增幅不到1%,但是,来自印度、韩国和肯尼亚的学生人数有显著增加。这份《2003年门户开放报告》(Open Doors2003)称,印度在美留学生人数居首位,中国居第二位。展开更多
The yield of direct-seeded rice(DSR)was constrained by inadequate grain filling.Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing y...The yield of direct-seeded rice(DSR)was constrained by inadequate grain filling.Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing yield.This study aimed to examine the effects of paclobutrazol seed soaking(PSS)on non-structural carbohydrate accumulation and grain enrichment in DSR,potentially providing a theoretical foundation for achieving high-yield DSR cultivation.The experiment utilized two rice varieties,Jiyujing(JYJ)and Jijing305(JJ305),with seeds soaked in paclobutrazol concentrations of 0 mg L−1 and 100 mg L−1.PSS demonstrated increased chlorophyll content,net photosynthetic rate,and leaf area,as well as an extended photosynthetic function period during the filling stage.It also elevated soluble sugar and starch contents in the flag leaf(during the filling stage)and stem sheath(after heading),decreased starch content in the top panicle while increasing it in the middle and lower panicle during the filling stage,and enhanced spikelet per unit area and seed setting rate,thereby improving DSR yield.In conclusion,PSS enhanced the photosynthetic capacity of DSR during the filling stage,coordinated the filling process of superior and inferior grains,maintained source-sink balance,and facilitated stable and orderly filling,ultimately resulting in improved yield.展开更多
Extremely high temperatures resulting from climate change have become a major challenge for increasing rice production.Therefore,our objective was to develop heat-tolerant aromatic rice varieties using the pedigree me...Extremely high temperatures resulting from climate change have become a major challenge for increasing rice production.Therefore,our objective was to develop heat-tolerant aromatic rice varieties using the pedigree method,focusing on selecting for seed-setting ability under extremely high temperatures along with the use of single nucleotide polymorphism/insertion and deletion(SNP/InDel)markers to improve aromatic properties and grain quality.Furthermore,the QTL-seq approach was utilized to identify QTLs for seed-setting rate in an F2 population subjected to heat stress.The candidate QTL regions were then aligned to confirm SNPs/InDels in synonymous F7 candidate heat-tolerant lines.The results revealed that four promising lines,namely 84-7-1-9,84-7-1-10,159-3-3-1,and 159-3-3-10,were classified as heat-tolerant with low amylose content.In addition,lines 84-7-1-9 and 84-7-1-10 were identified as aromatic rice encompassing the aroma gene(badh2).Regarding the QTL-seq results,the qSF2.1 region ranged from 311051 to 3929422 bp on chromosome 2,was identified based on the highest contrasting SNP index between the heat-susceptible and tolerant bulks.The candidate genes within this region include two genes related to heat shock proteins,three genes associated with pollen fertility,and four genes involved in heat stress and other abiotic stress responses.These genes are proposed as potential candidates for heat tolerance and could serve as targets in rice breeding programs aimed at enhancing heat tolerance.展开更多
Whole-grain rice has gained significant attention for its potential health benefits,particularly because of its antioxidant and antibacterial properties.Despite this growing interest,there remains a pressing need for ...Whole-grain rice has gained significant attention for its potential health benefits,particularly because of its antioxidant and antibacterial properties.Despite this growing interest,there remains a pressing need for a comprehensive evaluation of the methods used to analyze these bioactive compounds,considering the diversity of rice types and the influence of environmental factors.This review aims to provide an in-depth overview of recent analytical methods,particularly those employed over the last five years,to assess the antioxidant and antibacterial properties of rice.It focuses on commonly used antioxidant assays,such as the 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging activity assay,which encompasses both single electron transfer and hydrogen atom transfer mechanisms.Other antioxidant assays are also discussed,alongside methods used to evaluate antibacterial properties,including disc diffusion,well diffusion,broth or agar dilution,and advanced techniques such as flow cytofluorometric and bioluminescent assays.The review further explores the bacterial strains most frequently studied,including Bacillus cereus,Listeria monocytogenes,Escherichia coli,and Salmonella species.It highlights the key techniques and parameters employed to ascertain and quantify the antioxidant and antibacterial potential of rice,while critically analysing the strengths and limitations of these approaches.By synthesizing current methods and offering insights into their application,this review can be served as a guide for future research aimed at standardizing and improving the assessment of rice’s bioactive properties,paving the way for broader scientific and practical applications.展开更多
Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small...Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small auxin-up RNA gene, OsSAUR33, was found to be involved in the regulation of seed aging tolerance in rice. The expression of OsSAUR33 was significantly induced in aged seeds compared with unaged seeds during the seed germination phase. Accordingly, the disruption of OsSAUR33 significantly reduced seed vigor compared to the wild type(WT) in response to natural storage or artificial aging treatments. The rice OsSAUR33 gene promotes the vigor of aged seeds by enhancing their reactive oxygen species(ROS) level during seed germination, and the accumulation of ROS was significantly delayed in the aged seeds of Ossaur33 mutants in comparison with WT during seed germination. Hydrogen peroxide(H_(2)O_(2)) treatments promoted the vigor of aged seeds in various rice varieties. Our results provide timely theoretical and technical insights for the trait improvement of seed aging tolerance in rice.展开更多
Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in m...Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remain unclear.In this study,we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line NIL-Rf6 using hematoxylin-eosin staining and 4ʹ,6-diamidino-2-phenylindole(DAPI)staining.HL-CMS is characterized by gametophytic sterility,where the aborted pollen grains are empty,and the tapetal layer remains intact.Transmission electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage,revealing significant mitochondrial alterations,characterized by the formation of'large spherical mitochondria',occurred at the binucleate stage in the YA line.Additionally,metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid pathways.Notably,the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species(ROS)levels.Therefore,we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites,with impaired mitochondrial energy production and reduced flavonoid biosynthesis as the main causes of ROS accumulation and pollen abortion in rice.展开更多
In rice fields,rice plants usually grow alongside wild weeds and are attacked by various invertebrate species.Viruses are abundant in plants and invertebrates,playing crucial ecological roles in controlling microbial ...In rice fields,rice plants usually grow alongside wild weeds and are attacked by various invertebrate species.Viruses are abundant in plants and invertebrates,playing crucial ecological roles in controlling microbial abundance and maintaining community structures.To date,only 16 rice viruses have been documented in rice-growing regions.These viruses pose serious threats to rice production and have traditionally been identified only from rice plants and insect vectors by isolation techniques.Advances in next-generation sequencing(NGS)have made it feasible to discover viruses on a global scale.Recently,numerous viruses have been identified in plants and invertebrates using NGS technologies.In this review,we discuss viral studies in rice plants,invertebrate species,and weeds in rice fields.Many novel viruses have been discovered in rice ecosystems through NGS technologies,with some also detected using metatranscriptomic and small RNA sequencing.These analyses greatly expand our understanding of viruses in rice fields and provide valuable insights for developing efficient strategies to manage insect pests and virus-mediated rice diseases.展开更多
This study investigated the use of raspberry extract(RBE) for mitigating ethyl carbamate(EC) accumulation in Chinese rice wine(Huangjiu), a traditional fermented beverage. It focused on the addition of RBE to the ferm...This study investigated the use of raspberry extract(RBE) for mitigating ethyl carbamate(EC) accumulation in Chinese rice wine(Huangjiu), a traditional fermented beverage. It focused on the addition of RBE to the fermentation mash and its effects on EC levels. The results showed a significant reduction in EC production that could be attributed to RBE's role in altering urea and citrulline catabolism and inhibiting arginine metabolism, thus preventing EC precursors from reacting with ethanol. Additionally, RBE enhanced the rice wine's flavor profile, as shown by volatile component and amino acid analysis. This study also explored RBE's impact on the metabolism of arginine by Saccharomyces cerevisiae in a simulated fermentation environment, and found increased arginine, reduced urea and citrulline levels, altered enzyme activities, and gene expression changes in the arginine metabolism and transport pathways. In conclusion, the results clearly demonstrated RBE's efficacy in reducing the EC content in Chinese rice wine, offering valuable insights for EC reduction strategies.展开更多
The high labor demand during rice seedling cultivation and transplantation poses a significant challenge in advancing machine-transplanted rice cultivation.This problem may be solved by increasing the seeding rate dur...The high labor demand during rice seedling cultivation and transplantation poses a significant challenge in advancing machine-transplanted rice cultivation.This problem may be solved by increasing the seeding rate during seedling production while reducing the number of seedling trays.This study conducted field experiments from 2021 to 2022,using transplanting seedling ages of 10 and 15 days to explore the effects of 250,300,and 350 g/tray on the seedling quality,mechanical transplantation quality,yields,and economic benefits of rice.The commonly used combination of 150 g/tray with a 20-day seedling age in rice production was used as CK.The cultivation of seedlings under a high seeding rate and short seedling age significantly affected seedling characteristics,but there was no significant difference in seedling vitality compared to CK.The minimum number of rice trays used in the experiment was observed in the treatment of 350-10(300 g/tray and 10-day seedling age),only 152-155 trays ha^(-1),resulting in a 62%reduction in the number of trays needed.By increasing the seeding rate of rice,missed holes during mechanical transplantation decreased by 2.8 to 4%.The treatment of 300-15(300 g/tray and 15-day seedling age)achieved the highest yields and economic gains.These results indicated that using crop straw boards can reduce the application of seedling trays.On that basis,rice yields can be increased by raising the seeding rate and shortening the seedling age of rice without compromising seedling quality.展开更多
The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus(P)and potassium(K)solubilizing bacteria,enhancing the survival of plant growth-promoting rhizobacteria,and ultimately the sur...The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus(P)and potassium(K)solubilizing bacteria,enhancing the survival of plant growth-promoting rhizobacteria,and ultimately the survival of bacteria in the rhizosphere for better plant growth.A new biofilm-based formulation comprising the diazotrophic cyanobacteria Anabaena AMP2,P-solubilizing Bacillus megaterium var.phosphaticum PB1,and K-solubilizing Rhizobium pusense KRBKKM1 was tested for efficacy in rice.The growth medium with half-strength BG-11 medium supplemented with 3%glucose showed best for biofilm formation under in vitro conditions.Analysis of the methanolic extract of the cyanobacterial-bacterial biofilm(CBB)showed the activity of antioxidants,such as 2-methoxy phenol and pentadecane,which are proven to improve plant-microbe interactions and plant growth,respectively.Treatment of rice seeds with CBB extract at 100 mL/kg or 200 mL/kg showed significant enhancement in germination rate and seedling length.Therefore,a pot culture experiment with the CBB formulations was carried out,and different growth and yield parameters were recorded.Principal component analysis showed that plant growth,yield,soil dehydrogenase activity,and soil chlorophyll content were positively correlated with rice plants amended with vermiculite-based CBB at 2 kg/hm^(2) followed by a spray with aqueous CBB formulation at 5 mL/L at 15 and 30 d after rice transplanting grown with a 25%reduced level of nitrogen/phosphorus/potassium chemical fertilizers than the recommended dose.Further,Pearson correlation analysis showed that yield was positively correlated with soil dehydrogenase(r=0.92**)and soil chlorophyll content(r=0.96**).We concluded that CBB could be used as a novel biofilm-based bio-inoculant to increase rice productivity and crop fitness as a component in integrated nutrient management and sustainable organic farming strategies with reduced chemical fertilizers.展开更多
The Yangtze River Economic Belt is the main rice producing area in China.The rice industry chain is the agricultural pillar industry chain of this economic belt and it is the key to ensuring national food security and...The Yangtze River Economic Belt is the main rice producing area in China.The rice industry chain is the agricultural pillar industry chain of this economic belt and it is the key to ensuring national food security and promoting comprehensive rural revitalization.This study discusses the entire rice industry chain in the Yangtze River Economic Belt from the national rice production functional zones,agricultural product quality and safety,national famous and excellent new agricultural products,national specialty agricultural products,"China s good grain and oil"products,and national advantageous characteristic industrial clusters.Then,it discusses the geographical indications of rice and its products in this economic belt from geographical indication products,geographical indication trademarks,agricultural geographical indications,geographical indication standards,geographical indication special indications,national geographical indication product protection demonstration zones,and Chinese geographical indication products protected by the European Union.In addition,it analyzes the five main problems between geographical indications and public brands,such as the limited use of geographical indication specific signs and the imperfect intellectual property protection system for geographical indications.Finally,it proposes eight strategies,including promoting the high-quality development of the entire rice industry chain,creating a geographical indication regional public brand for rice and its products,and implementing geographical indication protection projects.展开更多
Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary ...Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four-factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019.The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13C stable isotope in-situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status.Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root-vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental,coupled with moderate field drying for root-vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop.展开更多
Ratoon rice,which refers to a second harvest of rice obtained from the regenerated tillers originating from the stubble of the first harvested crop,plays an important role in both food security and agroecology while r...Ratoon rice,which refers to a second harvest of rice obtained from the regenerated tillers originating from the stubble of the first harvested crop,plays an important role in both food security and agroecology while requiring minimal agricultural inputs.However,accurately identifying ratoon rice crops is challenging due to the similarity of its spectral features with other rice cropping systems(e.g.,double rice).Moreover,images with a high spatiotemporal resolution are essential since ratoon rice is generally cultivated in fragmented croplands within regions that frequently exhibit cloudy and rainy weather.In this study,taking Qichun County in Hubei Province,China as an example,we developed a new phenology-based ratoon rice vegetation index(PRVI)for the purpose of ratoon rice mapping at a 30 m spatial resolution using a robust time series generated from Harmonized Landsat and Sentinel-2(HLS)images.The PRVI that incorporated the red,near-infrared,and shortwave infrared 1 bands was developed based on the analysis of spectro-phenological separability and feature selection.Based on actual field samples,the performance of the PRVI for ratoon rice mapping was carefully evaluated by comparing it to several vegetation indices,including normalized difference vegetation index(NDVI),enhanced vegetation index(EVI)and land surface water index(LSWI).The results suggested that the PRVI could sufficiently capture the specific characteristics of ratoon rice,leading to a favorable separability between ratoon rice and other land cover types.Furthermore,the PRVI showed the best performance for identifying ratoon rice in the phenological phases characterized by grain filling and harvesting to tillering of the ratoon crop(GHS-TS2),indicating that only several images are required to obtain an accurate ratoon rice map.Finally,the PRVI performed better than NDVI,EVI,LSWI and their combination at the GHS-TS2 stages,with producer's accuracy and user's accuracy of 92.22 and 89.30%,respectively.These results demonstrate that the proposed PRVI based on HLS data can effectively identify ratoon rice in fragmented croplands at crucial phenological stages,which is promising for identifying the earliest timing of ratoon rice planting and can provide a fundamental dataset for crop management activities.展开更多
文摘根据美国非营利的教育和文化交流机构国际教育协会(Institute of International Education,IIE)2003年11月公布的年度报告,2002—2003学年,在美外国留学生总数增幅不到1%,但是,来自印度、韩国和肯尼亚的学生人数有显著增加。这份《2003年门户开放报告》(Open Doors2003)称,印度在美留学生人数居首位,中国居第二位。
基金National Key Research and Development Project of China(2022yfd1500501)Jilin Province Science and Technology Department Outstanding Young Talent Fund Project(20230508001RC).
文摘The yield of direct-seeded rice(DSR)was constrained by inadequate grain filling.Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing yield.This study aimed to examine the effects of paclobutrazol seed soaking(PSS)on non-structural carbohydrate accumulation and grain enrichment in DSR,potentially providing a theoretical foundation for achieving high-yield DSR cultivation.The experiment utilized two rice varieties,Jiyujing(JYJ)and Jijing305(JJ305),with seeds soaked in paclobutrazol concentrations of 0 mg L−1 and 100 mg L−1.PSS demonstrated increased chlorophyll content,net photosynthetic rate,and leaf area,as well as an extended photosynthetic function period during the filling stage.It also elevated soluble sugar and starch contents in the flag leaf(during the filling stage)and stem sheath(after heading),decreased starch content in the top panicle while increasing it in the middle and lower panicle during the filling stage,and enhanced spikelet per unit area and seed setting rate,thereby improving DSR yield.In conclusion,PSS enhanced the photosynthetic capacity of DSR during the filling stage,coordinated the filling process of superior and inferior grains,maintained source-sink balance,and facilitated stable and orderly filling,ultimately resulting in improved yield.
基金Agricultural Research Development Agency in Thailand for financing the study and the provision of this research.
文摘Extremely high temperatures resulting from climate change have become a major challenge for increasing rice production.Therefore,our objective was to develop heat-tolerant aromatic rice varieties using the pedigree method,focusing on selecting for seed-setting ability under extremely high temperatures along with the use of single nucleotide polymorphism/insertion and deletion(SNP/InDel)markers to improve aromatic properties and grain quality.Furthermore,the QTL-seq approach was utilized to identify QTLs for seed-setting rate in an F2 population subjected to heat stress.The candidate QTL regions were then aligned to confirm SNPs/InDels in synonymous F7 candidate heat-tolerant lines.The results revealed that four promising lines,namely 84-7-1-9,84-7-1-10,159-3-3-1,and 159-3-3-10,were classified as heat-tolerant with low amylose content.In addition,lines 84-7-1-9 and 84-7-1-10 were identified as aromatic rice encompassing the aroma gene(badh2).Regarding the QTL-seq results,the qSF2.1 region ranged from 311051 to 3929422 bp on chromosome 2,was identified based on the highest contrasting SNP index between the heat-susceptible and tolerant bulks.The candidate genes within this region include two genes related to heat shock proteins,three genes associated with pollen fertility,and four genes involved in heat stress and other abiotic stress responses.These genes are proposed as potential candidates for heat tolerance and could serve as targets in rice breeding programs aimed at enhancing heat tolerance.
文摘Whole-grain rice has gained significant attention for its potential health benefits,particularly because of its antioxidant and antibacterial properties.Despite this growing interest,there remains a pressing need for a comprehensive evaluation of the methods used to analyze these bioactive compounds,considering the diversity of rice types and the influence of environmental factors.This review aims to provide an in-depth overview of recent analytical methods,particularly those employed over the last five years,to assess the antioxidant and antibacterial properties of rice.It focuses on commonly used antioxidant assays,such as the 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging activity assay,which encompasses both single electron transfer and hydrogen atom transfer mechanisms.Other antioxidant assays are also discussed,alongside methods used to evaluate antibacterial properties,including disc diffusion,well diffusion,broth or agar dilution,and advanced techniques such as flow cytofluorometric and bioluminescent assays.The review further explores the bacterial strains most frequently studied,including Bacillus cereus,Listeria monocytogenes,Escherichia coli,and Salmonella species.It highlights the key techniques and parameters employed to ascertain and quantify the antioxidant and antibacterial potential of rice,while critically analysing the strengths and limitations of these approaches.By synthesizing current methods and offering insights into their application,this review can be served as a guide for future research aimed at standardizing and improving the assessment of rice’s bioactive properties,paving the way for broader scientific and practical applications.
基金supported by the Key-Area Research and Development Program of Guangdong Province,China(2022B0202060006)the National Natural Science Foundation of China(32201838,32272157,32172052,and 31971995)+2 种基金the Natural Science Foundation of Guangdong Province,China(2023A1515012052)the Project of Sanya Yazhou Bay Science and Technology City,China(SCKJJYRC-2022-87)the Double First-class Discipline Promotion Project,China(2021B10564001)
文摘Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small auxin-up RNA gene, OsSAUR33, was found to be involved in the regulation of seed aging tolerance in rice. The expression of OsSAUR33 was significantly induced in aged seeds compared with unaged seeds during the seed germination phase. Accordingly, the disruption of OsSAUR33 significantly reduced seed vigor compared to the wild type(WT) in response to natural storage or artificial aging treatments. The rice OsSAUR33 gene promotes the vigor of aged seeds by enhancing their reactive oxygen species(ROS) level during seed germination, and the accumulation of ROS was significantly delayed in the aged seeds of Ossaur33 mutants in comparison with WT during seed germination. Hydrogen peroxide(H_(2)O_(2)) treatments promoted the vigor of aged seeds in various rice varieties. Our results provide timely theoretical and technical insights for the trait improvement of seed aging tolerance in rice.
基金supported by the National Natural Science Foundation of China(Grant No.32472185)the Key Research and Development Program of Hubei Province,China(Grant No.2022BFE003)the Hubei Agriculture Science and Technology Innovation Center program,and the National Rice Industry Technology System,China(Grant No.CARS-01-07).
文摘Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remain unclear.In this study,we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line NIL-Rf6 using hematoxylin-eosin staining and 4ʹ,6-diamidino-2-phenylindole(DAPI)staining.HL-CMS is characterized by gametophytic sterility,where the aborted pollen grains are empty,and the tapetal layer remains intact.Transmission electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage,revealing significant mitochondrial alterations,characterized by the formation of'large spherical mitochondria',occurred at the binucleate stage in the YA line.Additionally,metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid pathways.Notably,the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species(ROS)levels.Therefore,we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites,with impaired mitochondrial energy production and reduced flavonoid biosynthesis as the main causes of ROS accumulation and pollen abortion in rice.
基金supported by the National Natural Science Foundation of China(Grant Nos.31972983 and 32072487)the Key Technology R&D Program of Zhejiang Province,China(Grant No.2021C02006)the Zhejiang Provincial Natural Science Foundation of China(Grant No.LY23C140001).
文摘In rice fields,rice plants usually grow alongside wild weeds and are attacked by various invertebrate species.Viruses are abundant in plants and invertebrates,playing crucial ecological roles in controlling microbial abundance and maintaining community structures.To date,only 16 rice viruses have been documented in rice-growing regions.These viruses pose serious threats to rice production and have traditionally been identified only from rice plants and insect vectors by isolation techniques.Advances in next-generation sequencing(NGS)have made it feasible to discover viruses on a global scale.Recently,numerous viruses have been identified in plants and invertebrates using NGS technologies.In this review,we discuss viral studies in rice plants,invertebrate species,and weeds in rice fields.Many novel viruses have been discovered in rice ecosystems through NGS technologies,with some also detected using metatranscriptomic and small RNA sequencing.These analyses greatly expand our understanding of viruses in rice fields and provide valuable insights for developing efficient strategies to manage insect pests and virus-mediated rice diseases.
基金supported by the National Natural Science Foundation of China(32202125)the Science and Technology Plan Project of Shaoxing City,China(2022A12003)the Zhejiang Provincial Natural Science Foundation,China(LY24C200004).
文摘This study investigated the use of raspberry extract(RBE) for mitigating ethyl carbamate(EC) accumulation in Chinese rice wine(Huangjiu), a traditional fermented beverage. It focused on the addition of RBE to the fermentation mash and its effects on EC levels. The results showed a significant reduction in EC production that could be attributed to RBE's role in altering urea and citrulline catabolism and inhibiting arginine metabolism, thus preventing EC precursors from reacting with ethanol. Additionally, RBE enhanced the rice wine's flavor profile, as shown by volatile component and amino acid analysis. This study also explored RBE's impact on the metabolism of arginine by Saccharomyces cerevisiae in a simulated fermentation environment, and found increased arginine, reduced urea and citrulline levels, altered enzyme activities, and gene expression changes in the arginine metabolism and transport pathways. In conclusion, the results clearly demonstrated RBE's efficacy in reducing the EC content in Chinese rice wine, offering valuable insights for EC reduction strategies.
基金funded by the Jiangsu Key Research Program,China(BE2022338)the Jiangsu Agricultural Science and Technology Innovation Fund,China(CX(23)3107)+3 种基金the Natural Science Foundation of the Jiangsu Higher Education Institutions,China(22KJB210004)the Jiangsu Province Agricultural Major Technology Collaborative Promotion Project,China(2022-ZYXT-04-1)the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX23_3569)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China.
文摘The high labor demand during rice seedling cultivation and transplantation poses a significant challenge in advancing machine-transplanted rice cultivation.This problem may be solved by increasing the seeding rate during seedling production while reducing the number of seedling trays.This study conducted field experiments from 2021 to 2022,using transplanting seedling ages of 10 and 15 days to explore the effects of 250,300,and 350 g/tray on the seedling quality,mechanical transplantation quality,yields,and economic benefits of rice.The commonly used combination of 150 g/tray with a 20-day seedling age in rice production was used as CK.The cultivation of seedlings under a high seeding rate and short seedling age significantly affected seedling characteristics,but there was no significant difference in seedling vitality compared to CK.The minimum number of rice trays used in the experiment was observed in the treatment of 350-10(300 g/tray and 10-day seedling age),only 152-155 trays ha^(-1),resulting in a 62%reduction in the number of trays needed.By increasing the seeding rate of rice,missed holes during mechanical transplantation decreased by 2.8 to 4%.The treatment of 300-15(300 g/tray and 15-day seedling age)achieved the highest yields and economic gains.These results indicated that using crop straw boards can reduce the application of seedling trays.On that basis,rice yields can be increased by raising the seeding rate and shortening the seedling age of rice without compromising seedling quality.
基金supported by the Researchers Supporting Project of King Saud University,Riyadh,Saudi Arabia(Grant No.RSP2025R358)Tamil Nadu Agricultural University,Coimbatore,India(Grant No.NRM-MDU-AGM-14-006).
文摘The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus(P)and potassium(K)solubilizing bacteria,enhancing the survival of plant growth-promoting rhizobacteria,and ultimately the survival of bacteria in the rhizosphere for better plant growth.A new biofilm-based formulation comprising the diazotrophic cyanobacteria Anabaena AMP2,P-solubilizing Bacillus megaterium var.phosphaticum PB1,and K-solubilizing Rhizobium pusense KRBKKM1 was tested for efficacy in rice.The growth medium with half-strength BG-11 medium supplemented with 3%glucose showed best for biofilm formation under in vitro conditions.Analysis of the methanolic extract of the cyanobacterial-bacterial biofilm(CBB)showed the activity of antioxidants,such as 2-methoxy phenol and pentadecane,which are proven to improve plant-microbe interactions and plant growth,respectively.Treatment of rice seeds with CBB extract at 100 mL/kg or 200 mL/kg showed significant enhancement in germination rate and seedling length.Therefore,a pot culture experiment with the CBB formulations was carried out,and different growth and yield parameters were recorded.Principal component analysis showed that plant growth,yield,soil dehydrogenase activity,and soil chlorophyll content were positively correlated with rice plants amended with vermiculite-based CBB at 2 kg/hm^(2) followed by a spray with aqueous CBB formulation at 5 mL/L at 15 and 30 d after rice transplanting grown with a 25%reduced level of nitrogen/phosphorus/potassium chemical fertilizers than the recommended dose.Further,Pearson correlation analysis showed that yield was positively correlated with soil dehydrogenase(r=0.92**)and soil chlorophyll content(r=0.96**).We concluded that CBB could be used as a novel biofilm-based bio-inoculant to increase rice productivity and crop fitness as a component in integrated nutrient management and sustainable organic farming strategies with reduced chemical fertilizers.
基金Supported by Social Science Foundation of Hubei Province (HBSKJJ20243227),Doctoral Initiation Project of Hubei University of Science and Technology (BK201819).
文摘The Yangtze River Economic Belt is the main rice producing area in China.The rice industry chain is the agricultural pillar industry chain of this economic belt and it is the key to ensuring national food security and promoting comprehensive rural revitalization.This study discusses the entire rice industry chain in the Yangtze River Economic Belt from the national rice production functional zones,agricultural product quality and safety,national famous and excellent new agricultural products,national specialty agricultural products,"China s good grain and oil"products,and national advantageous characteristic industrial clusters.Then,it discusses the geographical indications of rice and its products in this economic belt from geographical indication products,geographical indication trademarks,agricultural geographical indications,geographical indication standards,geographical indication special indications,national geographical indication product protection demonstration zones,and Chinese geographical indication products protected by the European Union.In addition,it analyzes the five main problems between geographical indications and public brands,such as the limited use of geographical indication specific signs and the imperfect intellectual property protection system for geographical indications.Finally,it proposes eight strategies,including promoting the high-quality development of the entire rice industry chain,creating a geographical indication regional public brand for rice and its products,and implementing geographical indication protection projects.
基金supported by the National Nature Science Foundation of China,the National Key Research and Development Program of China(302001109,2016YFD0300508,2017YFD0301602,2018YFD0301105)the Fujian and Taiwan Cultivation Resources Development and Green Cultivation Coordination Innovation Center,China(Fujian 2011 Project,2015-75)the Natural Science Foundation of Fujian Province,China(2022J01142)。
文摘Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four-factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019.The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13C stable isotope in-situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status.Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root-vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental,coupled with moderate field drying for root-vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop.
基金supported by the National Natural Science Foundation of China(42271360 and 42271399)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(CAST)(2020QNRC001)the Fundamental Research Funds for the Central Universities,China(2662021JC013,CCNU22QN018)。
文摘Ratoon rice,which refers to a second harvest of rice obtained from the regenerated tillers originating from the stubble of the first harvested crop,plays an important role in both food security and agroecology while requiring minimal agricultural inputs.However,accurately identifying ratoon rice crops is challenging due to the similarity of its spectral features with other rice cropping systems(e.g.,double rice).Moreover,images with a high spatiotemporal resolution are essential since ratoon rice is generally cultivated in fragmented croplands within regions that frequently exhibit cloudy and rainy weather.In this study,taking Qichun County in Hubei Province,China as an example,we developed a new phenology-based ratoon rice vegetation index(PRVI)for the purpose of ratoon rice mapping at a 30 m spatial resolution using a robust time series generated from Harmonized Landsat and Sentinel-2(HLS)images.The PRVI that incorporated the red,near-infrared,and shortwave infrared 1 bands was developed based on the analysis of spectro-phenological separability and feature selection.Based on actual field samples,the performance of the PRVI for ratoon rice mapping was carefully evaluated by comparing it to several vegetation indices,including normalized difference vegetation index(NDVI),enhanced vegetation index(EVI)and land surface water index(LSWI).The results suggested that the PRVI could sufficiently capture the specific characteristics of ratoon rice,leading to a favorable separability between ratoon rice and other land cover types.Furthermore,the PRVI showed the best performance for identifying ratoon rice in the phenological phases characterized by grain filling and harvesting to tillering of the ratoon crop(GHS-TS2),indicating that only several images are required to obtain an accurate ratoon rice map.Finally,the PRVI performed better than NDVI,EVI,LSWI and their combination at the GHS-TS2 stages,with producer's accuracy and user's accuracy of 92.22 and 89.30%,respectively.These results demonstrate that the proposed PRVI based on HLS data can effectively identify ratoon rice in fragmented croplands at crucial phenological stages,which is promising for identifying the earliest timing of ratoon rice planting and can provide a fundamental dataset for crop management activities.