Since the first report of the release of insects carrying a dominant lethal （RIDL） strategy, RIDL strains have been constructed in species including fruit flies and mosquitoes. However, in many insects, identificati...Since the first report of the release of insects carrying a dominant lethal （RIDL） strategy, RIDL strains have been constructed in species including fruit flies and mosquitoes. However, in many insects, identification of sterile and lethal genes needed to generate a RIDL strain is limited by the lack of molecular and genetic information. Here, we created RIDL strains of Drosophila melanogaster using RNA interference （RNAi） of the Pygopus （Pygo） gene, a key component of the Wingless/Wnt signaling pathway. In two transgenic lines, XDll and XD15, we verified lethality in the absence of tetracycline, but we were unable to demonstrate sex-specific lethality. We found that male XD15 adults maintained on medium without tetracycline had a longer lifespan than wild type. This RNAibased RIDL strain may therefore offer the advantages of a transgene that promotes the expression of two contrary actions at different life stages： lethality in larvae and prolonged lifespan in adults, actions that could work together to provide prolonged delivery of lethality by the RIDL system. Use of RNAi can facilitate the development and application of RIDL strategies in a wide range of species.展开更多
The main objectives were to (1) calculate the total volatile organic compounds (TVOCs) inhalation dose, (2) analyze the proportions of human’s inhaled contaminant dose from different sources, and (3) present a newly ...The main objectives were to (1) calculate the total volatile organic compounds (TVOCs) inhalation dose, (2) analyze the proportions of human’s inhaled contaminant dose from different sources, and (3) present a newly defined ratio of relative inhalation dose level (RIDL) to assess indoor air quality (IAQ). A user defined function based on CFD (computational fluid dynamics) was developed, which integrated human motion model with TVOCs emission model in a high sidewall air supply ventilation mode. Based on simulation results of 10 cases, it is shown that the spatial concentration distribution of TVOCs is affected by human motion. TVOCs diffusion characteristic of building material is the most effective way to impact the TVOCs inhalation dose. From the RIDL index, case A-2 has the most serious IAQ problem, while case D-1 is of the best IAQ.展开更多
遗传防控技术作为传统有害生物防控方式的替代策略,自20世纪50年代以来在世界各国受到广泛的关注,被认为是一类有利于人类健康、食品安全和农业可持续发展的害虫种群防控技术,同时也为日益严峻的生物入侵问题提供了解决方案.本文综述了...遗传防控技术作为传统有害生物防控方式的替代策略,自20世纪50年代以来在世界各国受到广泛的关注,被认为是一类有利于人类健康、食品安全和农业可持续发展的害虫种群防控技术,同时也为日益严峻的生物入侵问题提供了解决方案.本文综述了不育昆虫技术(sterile insect technique,SIT)、释放携带显性致死基因昆虫技术(release of insects carrying a dominant lethal,RIDL)和基因驱动技术(gene drive)的基本原理及部分应用案例,比较了不同技术的优势与限制因素,并介绍了相关研究中常见的几种基因整合策略,以期进一步改进害虫综合治理的基础研究和技术研发.展开更多
基金supported by the National Natural Science Foundation of China (30800721)
文摘Since the first report of the release of insects carrying a dominant lethal （RIDL） strategy, RIDL strains have been constructed in species including fruit flies and mosquitoes. However, in many insects, identification of sterile and lethal genes needed to generate a RIDL strain is limited by the lack of molecular and genetic information. Here, we created RIDL strains of Drosophila melanogaster using RNA interference （RNAi） of the Pygopus （Pygo） gene, a key component of the Wingless/Wnt signaling pathway. In two transgenic lines, XDll and XD15, we verified lethality in the absence of tetracycline, but we were unable to demonstrate sex-specific lethality. We found that male XD15 adults maintained on medium without tetracycline had a longer lifespan than wild type. This RNAibased RIDL strain may therefore offer the advantages of a transgene that promotes the expression of two contrary actions at different life stages： lethality in larvae and prolonged lifespan in adults, actions that could work together to provide prolonged delivery of lethality by the RIDL system. Use of RNAi can facilitate the development and application of RIDL strategies in a wide range of species.
基金Projects(2006BAJ02A08, 2006BAJ02A05) supported by the National Science and Technology Pillar Program Project during the 11th Five-Year Plan PeriodProject(2007-209) supported by the Excellent Youth Teacher of Ministry of Education of China
文摘The main objectives were to (1) calculate the total volatile organic compounds (TVOCs) inhalation dose, (2) analyze the proportions of human’s inhaled contaminant dose from different sources, and (3) present a newly defined ratio of relative inhalation dose level (RIDL) to assess indoor air quality (IAQ). A user defined function based on CFD (computational fluid dynamics) was developed, which integrated human motion model with TVOCs emission model in a high sidewall air supply ventilation mode. Based on simulation results of 10 cases, it is shown that the spatial concentration distribution of TVOCs is affected by human motion. TVOCs diffusion characteristic of building material is the most effective way to impact the TVOCs inhalation dose. From the RIDL index, case A-2 has the most serious IAQ problem, while case D-1 is of the best IAQ.
文摘遗传防控技术作为传统有害生物防控方式的替代策略,自20世纪50年代以来在世界各国受到广泛的关注,被认为是一类有利于人类健康、食品安全和农业可持续发展的害虫种群防控技术,同时也为日益严峻的生物入侵问题提供了解决方案.本文综述了不育昆虫技术(sterile insect technique,SIT)、释放携带显性致死基因昆虫技术(release of insects carrying a dominant lethal,RIDL)和基因驱动技术(gene drive)的基本原理及部分应用案例,比较了不同技术的优势与限制因素,并介绍了相关研究中常见的几种基因整合策略,以期进一步改进害虫综合治理的基础研究和技术研发.
