Condition indices study like RNA content,DNA content,RNA:DNA,RNA:protein,RNA:lipid was carried out in goby,Parachaeturichthys ocellatus from the creeks of Mumbai to assess its nutritional status in different mon...Condition indices study like RNA content,DNA content,RNA:DNA,RNA:protein,RNA:lipid was carried out in goby,Parachaeturichthys ocellatus from the creeks of Mumbai to assess its nutritional status in different months.The study was carried out from June 2010 to September 2011.The range of RNA content in male was 72-185.6μg/100 mg while in female was 82-145.46μg/100 mg.RNA content was high during spawning months.The DNA content showed slight variations with range of 22.56-39.31μg/100 mg in males and 25.20-32.52μg/100 mg in females.The range of ratio of RNA:DNA in males was 2.08-5.13 with an average of 3.74 while in female was 2.92-5.07 with an average of 3.99.The ratio above 2 indicates good condition.The RNA:protein showed an average of 0.0015 in males and 0.0017 in females while the average of RNA:lipid was 0.0176 in males and 0.0127 in females.RNA:protein and RNA:lipid showed the lowest values in post reproductive stages while it increased with the onset of reproductive cycles.The condition indices study showed that P.ocellatus was in good condition throughout the year and the creeks of Mumbai were suitable habitat for feeding and reproduction.展开更多
Telomeres are specialized structures at the ends of linear chromosomes that protect genome stability.The telomeric repeat-containing RNA(TERRA)that is transcribed from subtelomeric regions can invade into double-stran...Telomeres are specialized structures at the ends of linear chromosomes that protect genome stability.The telomeric repeat-containing RNA(TERRA)that is transcribed from subtelomeric regions can invade into double-stranded DNA regions and form RNA:DNA hybrid-containing structure called R-loop.In tumor cells,R-loop formation is closely linked to gene expression and the alternative lengthening of telomeres(ALT)pathway.Dysregulated R-loops can cause stalled replication forks and telomere instability.However,how R-loops are recognized and regulated,particularly at telomeres,is not well understood.We discovered that ILF3 selectively associates with telomeric R-loops and safeguards telomeres from abnormal homologous recombination.Knocking out ILF3 results in excessive R-loops at telomeres and triggers telomeric DNA damage responses.In addition,ILF3 deficiency disrupts telomere homeostasis and causes abnormalities in the ALT pathway.Using the proximity-dependent biotin identification(BioID)technology,we mapped the ILF3 interactome and discovered that ILF3 could interact with several DNA/RNA helicases,including DHX9.Importantly,ILF3 may aid in the resolution of telomeric R-loops through its interaction with DHX9.Our findings suggest that ILF3 may function as a reader of telomeric R-loops,helping to prevent abnormal homologous recombination and maintain telomere homeostasis.展开更多
文摘Condition indices study like RNA content,DNA content,RNA:DNA,RNA:protein,RNA:lipid was carried out in goby,Parachaeturichthys ocellatus from the creeks of Mumbai to assess its nutritional status in different months.The study was carried out from June 2010 to September 2011.The range of RNA content in male was 72-185.6μg/100 mg while in female was 82-145.46μg/100 mg.RNA content was high during spawning months.The DNA content showed slight variations with range of 22.56-39.31μg/100 mg in males and 25.20-32.52μg/100 mg in females.The range of ratio of RNA:DNA in males was 2.08-5.13 with an average of 3.74 while in female was 2.92-5.07 with an average of 3.99.The ratio above 2 indicates good condition.The RNA:protein showed an average of 0.0015 in males and 0.0017 in females while the average of RNA:lipid was 0.0176 in males and 0.0127 in females.RNA:protein and RNA:lipid showed the lowest values in post reproductive stages while it increased with the onset of reproductive cycles.The condition indices study showed that P.ocellatus was in good condition throughout the year and the creeks of Mumbai were suitable habitat for feeding and reproduction.
基金National Natural Science Foundation(Grant Nos.82271598,81871109,82071587,31930058,32330023 and 32170757)National Key Research and Development Program of China(2018YFA0107003)Guang Dong Basic and Applied Basic Research Foundation(2020A1515010462).
文摘Telomeres are specialized structures at the ends of linear chromosomes that protect genome stability.The telomeric repeat-containing RNA(TERRA)that is transcribed from subtelomeric regions can invade into double-stranded DNA regions and form RNA:DNA hybrid-containing structure called R-loop.In tumor cells,R-loop formation is closely linked to gene expression and the alternative lengthening of telomeres(ALT)pathway.Dysregulated R-loops can cause stalled replication forks and telomere instability.However,how R-loops are recognized and regulated,particularly at telomeres,is not well understood.We discovered that ILF3 selectively associates with telomeric R-loops and safeguards telomeres from abnormal homologous recombination.Knocking out ILF3 results in excessive R-loops at telomeres and triggers telomeric DNA damage responses.In addition,ILF3 deficiency disrupts telomere homeostasis and causes abnormalities in the ALT pathway.Using the proximity-dependent biotin identification(BioID)technology,we mapped the ILF3 interactome and discovered that ILF3 could interact with several DNA/RNA helicases,including DHX9.Importantly,ILF3 may aid in the resolution of telomeric R-loops through its interaction with DHX9.Our findings suggest that ILF3 may function as a reader of telomeric R-loops,helping to prevent abnormal homologous recombination and maintain telomere homeostasis.
