Abstract With the development of genome-wide sequencing technology, 195 types of functional long non-coding RNAs （lncRNAs） have so far been found, and their cellular roles are gradually being revealed. Now lncRNAs h...Abstract With the development of genome-wide sequencing technology, 195 types of functional long non-coding RNAs （lncRNAs） have so far been found, and their cellular roles are gradually being revealed. Now lncRNAs have become a hotspot in the life science. These small molecules exist in almost all higher eukaryotes, and have very important regulatory roles in these organisms. This review briefly summarizes recent progress in researches on antisense non-coding RNA in the INK4 locus.展开更多
目的建立口腔需氧菌和兼性菌的系统分离鉴定方法。方法招募20名口腔健康志愿者及8名口腔疾病患者,分别采集唾液、龈下菌斑、龈上菌斑和根尖周肉芽样本,采用形态鉴定、全自动生化鉴定仪及16s r RNA基因测序的多相分类方法,对口腔可培养...目的建立口腔需氧菌和兼性菌的系统分离鉴定方法。方法招募20名口腔健康志愿者及8名口腔疾病患者,分别采集唾液、龈下菌斑、龈上菌斑和根尖周肉芽样本,采用形态鉴定、全自动生化鉴定仪及16s r RNA基因测序的多相分类方法,对口腔可培养细菌进行分离鉴定。结果鉴定出口腔菌种63种,共175株,梅里埃生化鉴定与16s r RNA基因鉴定的符合度为22.39%,不符合的菌种以基因测序结果为准。链球菌属、放线菌属和葡萄球菌属检出率占前3位,菌种以咽峡炎链球菌、口腔放线菌、变异链球菌、缓症链球菌检出频率最高。慢性根尖周炎的咽峡炎链球菌检出率最高;放射性龋患者中,中间链球菌、金黄色葡萄球菌检出率较高;猛性龋患者中,变异链球菌远高于其他细菌。结论口腔需氧菌和兼性厌氧菌中以革兰阳性菌检出率最高。对于口腔细菌鉴定来说,梅里埃生化鉴定仪鉴定准确率不高,但可提供菌株生理生化指标,而全长的16s r RNA基因测序鉴定更为可靠。展开更多
Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. The ability of Brucella to survive and multiply in the hostile environment of host macrophages is essential for its viru...Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. The ability of Brucella to survive and multiply in the hostile environment of host macrophages is essential for its virulence. The cold shock protein Csp A plays an important role in the virulence of B. melitensis. To analyze the genes regulated by Csp A, the whole transcriptomes of B. melitensis NI?csp A and its parental wild-type strain, B. melitensis NI, were sequenced and analyzed using the Solexa/Illumina sequencing platform. A total of 446 differentially expressed genes were identified, including 324 up-regulated and 122 down-regulated genes. Numerous genes identified are involved in amino acid, fatty acid, nitrogen, and energy metabolism. Interestingly, all genes involved in the type IV secretion system and Lux R-type regulatory protein Vjb R were significantly down-regulated in NI?csp A. In addition, an effector translocation assay confirmed that the function of T4 SS in NI?csp A is influenced by deletion of the csp A gene. These results revealed the differential phenomena associated with virulence and metabolism in NI?csp A and NI, providing important information for understanding detailed Csp A-regulated interaction networks and Brucella pathogenesis.展开更多
目的对我国大陆地区首例懒惰狡诈球菌临床感染病例进行描述,并对分离的细菌进行病原学和耐药性分析,同时结合文献资料描述懒惰狡诈球菌引起病例的情况。方法查询该病例的临床信息以及该菌相关的文献信息进行病例描述,应用BD Phoenix?-10...目的对我国大陆地区首例懒惰狡诈球菌临床感染病例进行描述,并对分离的细菌进行病原学和耐药性分析,同时结合文献资料描述懒惰狡诈球菌引起病例的情况。方法查询该病例的临床信息以及该菌相关的文献信息进行病例描述,应用BD Phoenix?-100全自动细菌鉴定/药敏系统、Vitek 2 Compact全自动细菌鉴定药敏分析仪、布鲁克基质辅助激光解吸电离飞行时间质谱仪、16S r RNA基因测序对分离的细菌进行鉴定,应用纸片扩散法对该菌进行耐药性检测。结果该病例为头面部带状疱疹合并右眼带状疱疹性结膜炎患者,从其送检疱疹脓性分泌物中分离到大小两种菌落形态的革兰氏阳性球菌,通过两种全自动细菌鉴定/药敏系统鉴定分别鉴定为耳炎差异球菌(Alloiococcusotitis)和克氏库克菌(Micrococcus Kristinae),而质谱仪均未给出鉴定结果,通过16S r RNA基因测序和比对鉴定均为懒惰狡诈球菌(Dolosigranulum pigrum)。在检测的11种抗生素中,该菌对红霉素和克林霉素耐药。通过检索、整理和分析文献信息,我们揭示该菌感染与性别无关,易于感染低年龄组儿童和老年人,而且该菌能够引起的疾病谱广泛,易于引起脓毒血症和眼部疾病。结论懒惰狡诈球菌是一种潜在的致病菌。目前常见的商业化微生物鉴定系统均不能准确鉴定该菌,该菌鉴定需要依赖16S r RNA基因测序。展开更多
文摘Abstract With the development of genome-wide sequencing technology, 195 types of functional long non-coding RNAs （lncRNAs） have so far been found, and their cellular roles are gradually being revealed. Now lncRNAs have become a hotspot in the life science. These small molecules exist in almost all higher eukaryotes, and have very important regulatory roles in these organisms. This review briefly summarizes recent progress in researches on antisense non-coding RNA in the INK4 locus.
文摘目的建立口腔需氧菌和兼性菌的系统分离鉴定方法。方法招募20名口腔健康志愿者及8名口腔疾病患者,分别采集唾液、龈下菌斑、龈上菌斑和根尖周肉芽样本,采用形态鉴定、全自动生化鉴定仪及16s r RNA基因测序的多相分类方法,对口腔可培养细菌进行分离鉴定。结果鉴定出口腔菌种63种,共175株,梅里埃生化鉴定与16s r RNA基因鉴定的符合度为22.39%,不符合的菌种以基因测序结果为准。链球菌属、放线菌属和葡萄球菌属检出率占前3位,菌种以咽峡炎链球菌、口腔放线菌、变异链球菌、缓症链球菌检出频率最高。慢性根尖周炎的咽峡炎链球菌检出率最高;放射性龋患者中,中间链球菌、金黄色葡萄球菌检出率较高;猛性龋患者中,变异链球菌远高于其他细菌。结论口腔需氧菌和兼性厌氧菌中以革兰阳性菌检出率最高。对于口腔细菌鉴定来说,梅里埃生化鉴定仪鉴定准确率不高,但可提供菌株生理生化指标,而全长的16s r RNA基因测序鉴定更为可靠。
基金supported by the National Natural Science Foundation of China (31402197, 31372446)
文摘Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. The ability of Brucella to survive and multiply in the hostile environment of host macrophages is essential for its virulence. The cold shock protein Csp A plays an important role in the virulence of B. melitensis. To analyze the genes regulated by Csp A, the whole transcriptomes of B. melitensis NI?csp A and its parental wild-type strain, B. melitensis NI, were sequenced and analyzed using the Solexa/Illumina sequencing platform. A total of 446 differentially expressed genes were identified, including 324 up-regulated and 122 down-regulated genes. Numerous genes identified are involved in amino acid, fatty acid, nitrogen, and energy metabolism. Interestingly, all genes involved in the type IV secretion system and Lux R-type regulatory protein Vjb R were significantly down-regulated in NI?csp A. In addition, an effector translocation assay confirmed that the function of T4 SS in NI?csp A is influenced by deletion of the csp A gene. These results revealed the differential phenomena associated with virulence and metabolism in NI?csp A and NI, providing important information for understanding detailed Csp A-regulated interaction networks and Brucella pathogenesis.
文摘目的对我国大陆地区首例懒惰狡诈球菌临床感染病例进行描述,并对分离的细菌进行病原学和耐药性分析,同时结合文献资料描述懒惰狡诈球菌引起病例的情况。方法查询该病例的临床信息以及该菌相关的文献信息进行病例描述,应用BD Phoenix?-100全自动细菌鉴定/药敏系统、Vitek 2 Compact全自动细菌鉴定药敏分析仪、布鲁克基质辅助激光解吸电离飞行时间质谱仪、16S r RNA基因测序对分离的细菌进行鉴定,应用纸片扩散法对该菌进行耐药性检测。结果该病例为头面部带状疱疹合并右眼带状疱疹性结膜炎患者,从其送检疱疹脓性分泌物中分离到大小两种菌落形态的革兰氏阳性球菌,通过两种全自动细菌鉴定/药敏系统鉴定分别鉴定为耳炎差异球菌(Alloiococcusotitis)和克氏库克菌(Micrococcus Kristinae),而质谱仪均未给出鉴定结果,通过16S r RNA基因测序和比对鉴定均为懒惰狡诈球菌(Dolosigranulum pigrum)。在检测的11种抗生素中,该菌对红霉素和克林霉素耐药。通过检索、整理和分析文献信息,我们揭示该菌感染与性别无关,易于感染低年龄组儿童和老年人,而且该菌能够引起的疾病谱广泛,易于引起脓毒血症和眼部疾病。结论懒惰狡诈球菌是一种潜在的致病菌。目前常见的商业化微生物鉴定系统均不能准确鉴定该菌,该菌鉴定需要依赖16S r RNA基因测序。
