非编码小RNA(non-coding small RNA,sRNA)是原核生物中最重要的一类转录后调节因子,一般通过碱基互补配对调节靶标基因表达。sRNA及其调控的靶标基因构成了一个复杂庞大的调控网络,在微生物的各个生理过程发挥着关键性的调控作用,包括...非编码小RNA(non-coding small RNA,sRNA)是原核生物中最重要的一类转录后调节因子,一般通过碱基互补配对调节靶标基因表达。sRNA及其调控的靶标基因构成了一个复杂庞大的调控网络,在微生物的各个生理过程发挥着关键性的调控作用,包括毒力和致病性。本文就原核sRNA的产生和功能机制以及sRNA互作网络的鉴定展开综述,以期为发现新的sRNA并阐明它们的调控功能提供新的研究思路。展开更多
The pervasive transcription of the genome creates many types of non-coding RNAs(nc RNAs).However,we know very little regarding the functions and the regulatory mechanisms of these nc RNAs.Exploring the interactions of...The pervasive transcription of the genome creates many types of non-coding RNAs(nc RNAs).However,we know very little regarding the functions and the regulatory mechanisms of these nc RNAs.Exploring the interactions of RNA and RNA binding proteins(RBPs) is vital because it can allow us to truly understand how these nc RNAs behave in vivo.High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation(HITS-CLIP or CLIP-seq) and its variants have been successfully used as systemic techniques to study RBP binding sites.In this review,we will explain the major differences between the CLIP techniques,summarize successful applications of these techniques,discuss limitations of CLIP,present some suggested solutions and project their promising future roles in studying the RNA world.展开更多
文摘非编码小RNA(non-coding small RNA,sRNA)是原核生物中最重要的一类转录后调节因子,一般通过碱基互补配对调节靶标基因表达。sRNA及其调控的靶标基因构成了一个复杂庞大的调控网络,在微生物的各个生理过程发挥着关键性的调控作用,包括毒力和致病性。本文就原核sRNA的产生和功能机制以及sRNA互作网络的鉴定展开综述,以期为发现新的sRNA并阐明它们的调控功能提供新的研究思路。
基金supported by the National Natural Science Foundation of China(31200593,31230042)the Guangdong Natural Science Foundation (S2011040001760)+1 种基金the Fundamental Research Funds for the Central Universities (13lgpy40)the National Basic Research Program of China (2011CB811300)
文摘The pervasive transcription of the genome creates many types of non-coding RNAs(nc RNAs).However,we know very little regarding the functions and the regulatory mechanisms of these nc RNAs.Exploring the interactions of RNA and RNA binding proteins(RBPs) is vital because it can allow us to truly understand how these nc RNAs behave in vivo.High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation(HITS-CLIP or CLIP-seq) and its variants have been successfully used as systemic techniques to study RBP binding sites.In this review,we will explain the major differences between the CLIP techniques,summarize successful applications of these techniques,discuss limitations of CLIP,present some suggested solutions and project their promising future roles in studying the RNA world.
