DNAzymes with RNA-cleaving activity have been widely used as biosensing and bioimaging tools for detection of metal ions.Despite the achievements,DNAzyme-based biosensors sometime suffer from false positive signals an...DNAzymes with RNA-cleaving activity have been widely used as biosensing and bioimaging tools for detection of metal ions.Despite the achievements,DNAzyme-based biosensors sometime suffer from false positive signals and unexpected off-target turn-on in biological environments,which are likely due to the unstable nature of the RNA site.Ways to control DNAzyme activity in order to improve the sensing performance remain a significant challenge.To meet the challenge,there is growing interest to develop synthetic strategies that can cage native DNAzyme under undesired conditions and reactivate it in target environment in order to function in a controlled manner.A variety of caging-decaging strategies have been developed to realize spatiotemporal control of the DNAzyme activity,improving its specificity and sensitivity as well as extending its application regimes.In this review,we focus on strategies to regulate the catalytic activity of DNAzyme,highlight the nucleic acid modification chemistries,and summarize three strategies to cage DNAzyme functions.Examples of using caged DNAzyme for bio-applications have also been reviewed in detail.Finally,we provide our perspectives on the potential challenges and opportunities of this emerging research topic that could advance the DNAzyme field.展开更多
基金supported by the National Natural Science Foundation of China(No.21877032)the Hunan Province Talented Young Scientists Program,China(Nos.2019RS2021,2019RS2023)+2 种基金the Open Research Fund Program of the State Key Laboratory of Analytical Chemistry for Life Sciences,China(No.SKLACLS2102)the Scientific Research Program of Shenzhen Institute of Synthetic Biology,China(No.DWKF20210005)the Fundamental Research Funds for the Central Universities,China.
文摘DNAzymes with RNA-cleaving activity have been widely used as biosensing and bioimaging tools for detection of metal ions.Despite the achievements,DNAzyme-based biosensors sometime suffer from false positive signals and unexpected off-target turn-on in biological environments,which are likely due to the unstable nature of the RNA site.Ways to control DNAzyme activity in order to improve the sensing performance remain a significant challenge.To meet the challenge,there is growing interest to develop synthetic strategies that can cage native DNAzyme under undesired conditions and reactivate it in target environment in order to function in a controlled manner.A variety of caging-decaging strategies have been developed to realize spatiotemporal control of the DNAzyme activity,improving its specificity and sensitivity as well as extending its application regimes.In this review,we focus on strategies to regulate the catalytic activity of DNAzyme,highlight the nucleic acid modification chemistries,and summarize three strategies to cage DNAzyme functions.Examples of using caged DNAzyme for bio-applications have also been reviewed in detail.Finally,we provide our perspectives on the potential challenges and opportunities of this emerging research topic that could advance the DNAzyme field.
