Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutat...Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases(RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were2.6% and 2.2% in the Arabidopsis FLOWERING TIME(At FT) and TERMINAL FLOWER 1(At TFL1)loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.展开更多
In a very recent article published in Science,Altae-Tran et al.recon-structed the evolution of CRISPR-Cas9 systems and traced their ances-tors to unique groups of transposons(Altae-Tran et al.,2021).These transposable...In a very recent article published in Science,Altae-Tran et al.recon-structed the evolution of CRISPR-Cas9 systems and traced their ances-tors to unique groups of transposons(Altae-Tran et al.,2021).These transposable elements encode RNA-guided nucleases that show strong potential for developing novel biotechnologies.Structural domains of these nucleases serve as useful building blocks for engineering novel RNA-guided nucleases via synthetic biology to strongly inspire the de-velopment of novel and precision genome-editing tools.展开更多
基金financially supported by the National Natural Science Foundation of China(No.31361140364)the National Major Project for Developing New GM Crops(No.2016ZX080009-001)the Agricultural Science and Technology Innovation Program(ASTIP)of CAAS to Chuanxiao Xie
文摘Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases(RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were2.6% and 2.2% in the Arabidopsis FLOWERING TIME(At FT) and TERMINAL FLOWER 1(At TFL1)loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.
基金supported by grants from the National Key R&D Program of China(2020YFA0906800 to QS)the National Natural Science Foundation of China(Grant No.31771380 to QS)State Kay Laboratory of Microbial Technology at Shandong University.
文摘In a very recent article published in Science,Altae-Tran et al.recon-structed the evolution of CRISPR-Cas9 systems and traced their ances-tors to unique groups of transposons(Altae-Tran et al.,2021).These transposable elements encode RNA-guided nucleases that show strong potential for developing novel biotechnologies.Structural domains of these nucleases serve as useful building blocks for engineering novel RNA-guided nucleases via synthetic biology to strongly inspire the de-velopment of novel and precision genome-editing tools.
