针对H9亚型禽流感HA基因保守序列设计引物,建立基于SYBR GreenⅠ检测模式的荧光定量RT-PCR（real-ti me RT-PCR,RRT-PCR）,最低检测限为8.33×102拷贝质粒DNA,与常规PCR相比,灵敏度高出100倍。扩增产物的熔解曲线分析只出现1个单特...针对H9亚型禽流感HA基因保守序列设计引物,建立基于SYBR GreenⅠ检测模式的荧光定量RT-PCR（real-ti me RT-PCR,RRT-PCR）,最低检测限为8.33×102拷贝质粒DNA,与常规PCR相比,灵敏度高出100倍。扩增产物的熔解曲线分析只出现1个单特异峰,无引物二聚体,Tm值为（83.83±0.22）℃,组内变异系数为0.52%~1.48%,组间变异系数为0.71%~2.21%,可重复性好;对H5亚型禽流感病毒、鸭肝炎病毒、鸭瘟病毒、鸭源禽1型副粘病毒无扩增;检测速度快,从样本处理到报告结果仅需4.5 h。展开更多
<strong>Introduction:</strong> Coronavirus Disease 2019 (COVID-19) is an infectious respiratory disease caused by SARS-CoV-2. The disease was first broke out in Wuhan City, Hubei Province, China and subseq...<strong>Introduction:</strong> Coronavirus Disease 2019 (COVID-19) is an infectious respiratory disease caused by SARS-CoV-2. The disease was first broke out in Wuhan City, Hubei Province, China and subsequently spread to all countries and was considered by WHO as a worldwide pandemic. This study is aimed to determine the epidemiology of SARS-CoV-2 and the presence of UK variants in Zintan city of Libya taking some risk factors into account. <strong>Methods:</strong> In a cross-sectional retrospective study, a total of 15486 nasopharyngeal swabs were collected from COVID-19 suspected patients, travelers and people need disease-free certificates for hospital admission, etc. The samples were collected during the period from August 2020 to June 2021 and tested using real-time RT-PCR (rRT-PCR) kits for SARS-CoV-2 and UK variants. Age groups, sex, and monthly weather were considered as risk factors. <strong>Results:</strong> The positivity rate of COVID-19 in Zintan city was estimated to be (3891;25.12%) for the period from August 2020 to June 2021. Females showed significantly (p < 0.05) higher positivity rate (2100;54%) as compared to males (1791;46%). Out of the 3891 positive cases, 52 were deceased. The Case Fatality Rate (CFR) was 1.33 recorded significantly in cases aged ≥ 65 years which was higher in males (56.66%) than females (43.33%). The peak of the first wave of infection was recorded in October 2020 (590;15.15%) whereas the peak of the second wave of infection was recorded in April 2021 (727;18.71%). The positivity rate was decreased as the temperature increased. UK variant is detected firstly in May 2021 with the percentage of 6.2% of tested samples.<strong> Conclusions:</strong> Health Authorities are encouraged to continue implementing the control measures during the decrease phase of infection to stop transmission of the virus in the next wave. Early detection of new variants and studying their genetic characteristics play a valuable role in prevention and control.展开更多
文摘针对H9亚型禽流感HA基因保守序列设计引物,建立基于SYBR GreenⅠ检测模式的荧光定量RT-PCR（real-ti me RT-PCR,RRT-PCR）,最低检测限为8.33×102拷贝质粒DNA,与常规PCR相比,灵敏度高出100倍。扩增产物的熔解曲线分析只出现1个单特异峰,无引物二聚体,Tm值为（83.83±0.22）℃,组内变异系数为0.52%~1.48%,组间变异系数为0.71%~2.21%,可重复性好;对H5亚型禽流感病毒、鸭肝炎病毒、鸭瘟病毒、鸭源禽1型副粘病毒无扩增;检测速度快,从样本处理到报告结果仅需4.5 h。
文摘<strong>Introduction:</strong> Coronavirus Disease 2019 (COVID-19) is an infectious respiratory disease caused by SARS-CoV-2. The disease was first broke out in Wuhan City, Hubei Province, China and subsequently spread to all countries and was considered by WHO as a worldwide pandemic. This study is aimed to determine the epidemiology of SARS-CoV-2 and the presence of UK variants in Zintan city of Libya taking some risk factors into account. <strong>Methods:</strong> In a cross-sectional retrospective study, a total of 15486 nasopharyngeal swabs were collected from COVID-19 suspected patients, travelers and people need disease-free certificates for hospital admission, etc. The samples were collected during the period from August 2020 to June 2021 and tested using real-time RT-PCR (rRT-PCR) kits for SARS-CoV-2 and UK variants. Age groups, sex, and monthly weather were considered as risk factors. <strong>Results:</strong> The positivity rate of COVID-19 in Zintan city was estimated to be (3891;25.12%) for the period from August 2020 to June 2021. Females showed significantly (p < 0.05) higher positivity rate (2100;54%) as compared to males (1791;46%). Out of the 3891 positive cases, 52 were deceased. The Case Fatality Rate (CFR) was 1.33 recorded significantly in cases aged ≥ 65 years which was higher in males (56.66%) than females (43.33%). The peak of the first wave of infection was recorded in October 2020 (590;15.15%) whereas the peak of the second wave of infection was recorded in April 2021 (727;18.71%). The positivity rate was decreased as the temperature increased. UK variant is detected firstly in May 2021 with the percentage of 6.2% of tested samples.<strong> Conclusions:</strong> Health Authorities are encouraged to continue implementing the control measures during the decrease phase of infection to stop transmission of the virus in the next wave. Early detection of new variants and studying their genetic characteristics play a valuable role in prevention and control.
