5种红山茶叶绿素荧光特性的比较研究

在大四条件下 ,通过对五种红山茶的叶绿素荧光特性研究 ,结果表明 ,不同亚组、同一亚组不同系之间的叶绿素荧光参数值存在差异 ,且亚组间大于亚组内。初始荧光 (FO)、最大荧光 (Fm)、RSII最大光化学效率 (Fv/ Fm )、PSII实际光化学效率 (Yield/Φ PSII)、表观电子传递速率 (ETR)的日变化与光强有关 ,其中 ,Fm、Fv/ Fm、Yield随光强的变化呈相同的变化趋势 ,呈单谷曲线 ;而ETR则相反呈单峰曲线 ;FO呈双峰曲线。不同树种的荧光参数日变化幅度存在差异。

基于SMRT测序技术的16S rRNA基因全长测序及其分析方法

被称为第三代测序技术的单分子测序是最近几年发展起来的高通量测序技术。其中,由Pacbio Bio Sciences公司开发的单分子实时测序技术(SMRT)是最先商用的技术。SMRT测序技术通过对模板序列循环测序产生环形一致序列(CCS),成功克服第三代测序技术准确率低的弊病。通过SMRT测序技术,科学家可以更深入准确地探究复杂环境微生物的结构和功能。介绍SMRT测序技术在微生物16S rRNA基因测序中的优势和劣势,并就基于SMRT测序技术所得的全长16S rRNA基因序列的质量控制、错误序列排除、聚类和注释分析等重要分析环节进行概述,同时,提出利用SMRT测序技术研究复杂环境微生物可能存在的问题及其解决方法,期望能为研究人员提供参考。

Microphysical Properties of Convective Clouds in Summer over the Tibetan Plateau from SNPP/VIIRS Satellite Data

The Tibetan Plateau (TP) plays an important role in formation and development of the East Asian atmospheric circulation, climate variability, and disastrous weathers in China. Among the many topics on TP meteorology, it is critical to understand the microphysical characteristics of clouds over the TP;however, observations of the cloud microphysics in this area are insufficient mainly due to sparse stations and limited cloud physical data. The Visible Infrared Imaging Radiometer Suite (VⅡRS), onboard the Suomi National Polar-orbiting Partnership (SNPP) satellite, has an improved imaging spectroradiometer with 17 channels of 750-m moderate resolution and 5 channels of 375-m image resolution. The high-resolution instrument has an advantage for observing the small or initial convective clouds. Based on the methodologies that we proposed before for retrieving cloud microphysical properties from SNPP, an automated mapping software package named Automatic Mapping of Convective Clouds (AMCC) has been developed at the scale of satellite swath. The properties of convective clouds are retrieved by AMCC and their values are averaged over 0.33°×0.33° grids based on the SNPP/VⅡRS satellite data over the TP during the summers of 2013-17. The results show that:(1) the temperature of lifting condensation level (TLCL) at Naqu meteorological station and the cloud base temperature (Tb) retrieved from VⅡRS are linearly correlated, with a correlation coefficient of 0.87 and standard deviation (STD) of 3.0℃;(2) convective clouds over the TP have the following macro-and microphysical properties. First, the cloud base temperature (Tb) is about -5℃, the cloud base height above the ground (Hb) ranges between 1800 and 2200 m, and the cloud water content is low. Second, the cloud condensation nuclei concentration (NCCN) is between 200 and 400 mg-1 with 0.7% in maximum supersaturation (Smax);consequently, the condensation growth of water cloud droplet with less NCCN and higher Smax is fast. Third, because the precipitation initiation depth (D14) varies within 1500-2000 m and 500-1000 m at the Yarlung Zangbo River basin and southern Tibet, respectively, the clouds over these areas are more prone to precipitation. Fourth, mean height of the cloud top above sea level (Htop) is between 10 and 13 km, but the cloud depth (Dcld) is rather small, which is about 5000 m in southern TP and gradually reduces to 2500 m in northern TP. Fifth, the glaciation temperature (Tg) ranges from -30℃ in central and southern TP to -25℃ in northern TP, which, combined with the warmer Tg and the Tb less than 0℃, leads to the domination of ice process in the clouds;(3) the macro-and microphysical properties of convective clouds over the TP explain why rainfall there is frequent and lasts over a short time with small amount and large rain drops.