婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体及RSV抗体检测分析

目的分析婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体和RSV抗体检测结果,为临床儿童呼吸道疾病提供诊断依据。方法选择我院2007年12月～2009年12月婴幼儿急性下呼吸道感染和支气管哮喘患儿共100例作为研究对象,检测抗肺炎支原体、肺炎衣原体和RSV抗体情况,并进行统计学分析。结果 100例婴幼儿急性下呼吸道感染和支气管哮喘患儿中,血清抗肺炎支原体抗体、抗肺炎衣原体抗体、抗RSV抗体阳性率分别为37%、21%、44%,而同期100例非呼吸道疾病患儿血清抗肺炎支原体抗体、抗肺炎衣原体抗体、抗RSV抗体的阳性率明显低于观察组,具有显著性差异(P<0.01)。且不同年龄段肺炎支原体、肺炎衣原体及呼吸道合胞病毒的感染率明显不同,其中抗肺炎支原体、肺炎衣原体抗体中新生儿阳性率为0。主要以6岁以上年龄段的儿童感染率高。结论婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体和RSV抗体检测有利于婴幼儿呼吸道疾病的诊断、治疗,具有重要的临床意义。

婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体和RSV抗体检测分析

目的：了解婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体和RSV抗体水平。方法：采用回顾性分析方法,将接受间接免疫荧光法检测的急性下呼吸道感染和支气管哮喘的婴幼儿患儿120例和对照组100例无相关呼吸道疾病婴幼儿的血清抗肺炎支原体（MP）抗体、抗肺炎衣原体（CP）抗体和抗呼吸道合胞病毒（RSV）抗体检测结果进行对比分析。结果：①观察组血清抗MP抗体阳性率最高,为35.83%,抗CP抗体阳性率最低,为22.50%,与对照组相比差异有统计学意义（χ^2=14.78,P〈0.05;χ^2=7.22,P〈0.05）。②婴幼儿患儿各年龄段中MP IgM、CP IgM和RSV IgM分布情况,血清MP IgM和CP IgM阳性率在13岁组最高,RSVIgM阳性率30天1岁组中最高。③抗RSV抗体在支气管哮喘中阳性率最高分别为55.56%,抗MP抗体阳性率在非典型肺炎中最高,为46.27%。④观察组患儿MP抗体、抗CP抗体和抗RSV抗体检测,单项指标的临床诊断实验评价抗MP抗体的敏感性和真实性最高,分别为37.66%和54.00%。结论：抗MP抗体、抗CP抗体和抗RSV抗体的检测是诊断婴幼儿急性下呼吸道感染和支气管哮喘中重要的参考指标。

Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing

Resveratrol(3,5,4’-trihydroxystilbene,RSV) has been widely used in mammalian cells,but whether it can be used during freezing boar semen is still unknown.The effects of RSV treatment during boar semen freezing on its anti-freezing ability,apoptosis,and possible apoptotic pathways were observed in this study.Sperm motility,mitochondrial membrane potential(ΔΨm),adenosine triphosphate(ATP) content,terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick-end labeling(TUNEL)-positive apoptotic state,and messenger RNA(mRNA) expression levels of apoptotic genes involved in different apoptotic pathways after freezing with or without RSV treatment were tested.The results showed that:(1) Compared with fresh sperm,the motility,normal acrosome rate,and plasma membrane integrity rate of frozen boar sperm decreased significantly(P<0.05),and RSV did not significantly increase the sperm motility(0.44 vs.0.40,P>0.05),but it did significantly improve the normal acrosome rate(57.65% vs.47.00%,P<0.05) and plasma membrane integrity rate(46.67% vs.38.85%,P<0.05).(2) After freezing,most boar sperm showed low mitochondrial ΔΨm.RSV treatment could increase the rate of high mitochondrial ΔΨm of boar sperm.(3) RSV treatment significantly decreased reactive oxygen species(ROS) levels(58.65% vs.88.41%,P<0.05)and increased the ATP content(0.49 μmol/L vs.0.25 μmol/L,P<0.05) of boar sperm during freezing.(4) The apoptotic rate of the freezing group(80.41%) with TUNEL detection increased significantly compared to the fresh group(9.70%,P<0.05),and RSV treatment greatly decreased the apoptotic rate(68.32%,P<0.05).(5) Real-time polymerase chain reaction(RT-PCR) showed that not only the genes from the death receptor-mediated apoptotic pathway(tumor necrosis factor-α(TNF-α),Fas ligand(FasL),and Caspase-8),but also the genes from the mitochondria-mediated apoptotic pathway(manganese superoxide dismutase(MnSOD),B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax),and Caspase-9) were both significantly changed after freezing.RSV treatment during freezing greatly changed their expression levels.Although RSV treatment during boar semen freezing did not significantly increase motility after thawing,it still played an efficient antioxidant role,which could enhance the mitochondrial function and decrease the apoptotic level induced by both the death receptor-and mitochondria-mediated apoptotic pathways.