富含抗RSV-IgG血浆的筛选及对小儿重症RSV肺炎的治疗

目的：为了观察富含抗ＲＳＶ－ＩｇＧ血浆对小儿重症合胞病毒（ＲＳＶ）肺炎的辅助治疗作用。方法：应用间接ＥＬＩＳＡ法测定特异性ＩｇＧ效价，对供血者进行筛选，并应用于小儿重症ＲＳＶ肺炎的辅助治疗。结果：富含特异性ＩｇＧ新鲜血浆比普通新鲜血浆有更为明显的辅助治疗作用。结论：富含特异性ＩｇＧ新鲜血浆对小儿重症ＲＳＶ肺炎的辅助治疗作用肯定。

应用纯化的特异性RSV-IgG治疗RSV感染

本文介绍纯化的特异性RSV-IgG治疗RSV感染的途径、剂量和疗效,动物实验揭示鼻腔局部给予远优于全身给予。

Sero-Epidemiological Study of Respiratory Syncytial Virus

Background: Respiratory syncytial virus (RSV) is one of the major viruses that cause respiratory infections in all generations, not only in neonates and infants. There is a limited number of reports on serological epidemiology of RSV subgroups A and B. Neutralizing test (NT) antibody reflects protective immunity but bothersome. Sero-epidemiological study should be performed using practical NT method. Methods: Two wild-type viruses subgroups A and B, isolated in 2013, and the Long strain was used as the challenge viruses. NT antibody with 100% inhibition of cytopathic effect (CPE) was examined. A total of 91 serum samples obtained from 0 to 12 years subjects without RSV infection who visited our hospital with some health problems and 121 sera obtained from healthy subjects in different age groups were used. Serological epidemiology of subgroups A and B was investigated in this study using new NT methods. Results: 1) A simple and practical NT method was developed. 2) The NT antibody titer was lowest in <1 year of age (5 × 21.70 ± 2.03 against subgroup A and 5 × 20.85 ± 1.31 against subgroup B) and increased in 3 years of age or older, and high antibody titers were maintained during school age. 3) A slight difference was observed in the NT antibody titers against subgroups A and Bin young children <3 years, but not after 3 years of age, reflecting the repeated infections. 4) Specific IgG antibody against RSV was measured. The IgG EIA values decreased with age. No association was observed between IgG EIA and NT titers. Conclusions: A simple NT assay method was developed in the present study. By the age of 3 years, high NT antibody titers were observed and maintained until 12 years. The IgG (EIA) values decreased with age. No association was observed between IgG (EIA) and NT titers.

不同疾病人群血清中呼吸道合胞病毒特异性抗体IgM、IgG和IgA的分析

目的探讨呼吸道合胞病毒(RSV)特异性抗体IgM、IgG和IgA在不同疾病人群中的分布情况。方法运用荧光定量聚合酶链反应技术(FQ-PCR)筛选出50例咽拭子呼吸道合胞病毒(RSV)阳性有呼吸道感染症状的患儿作为观察组,同时以125例无呼吸道感染症状的不同疾病血清标本作为对照组。采用酶联免疫吸附试验(ELISA)检测患儿血清中的特异性抗体IgM、IgG和IgA水平。结果 IgM在观察组检出率仅为24.0%,在支气管肺炎和急性支气管炎中的检出率最高,分别为46.0%和58.7%;IgM、IgG和IgA在对照组不同疾病中检出率分别为6.4%、96.0%和20.0%;IgG在母婴对照组检出率均为100.0%,且抗体水平两组基本一致;IgA在<0.5岁、>0.5~1岁、>1~5岁和>5岁年龄段检出率分别为7.5%、27.8%、84.4%和97.6%。结论 IgM只能作为RSV早期感染的辅助诊断指标;母婴IgG抗体存在垂直传播,可能为机体保护性抗体;IgA随年龄增长越容易产生;RSV在儿童中主要引起支气管肺炎和急性支气管炎,且在不同疾病及不同年龄段存在较高隐性感染。