Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3′ coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology...Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3′ coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes.展开更多
The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was e...The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.展开更多
文摘Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3′ coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes.
文摘The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.
