对遗传背景清晰且具有显著表型差异的海带6个栽培品系(CUL002,CUL860,CUL1170,CUL017,CUL018,CUL901)和长海带(L.longissia)的ITS区进行了PCR扩增和序列测定,并分析了8个种17个品系(其中7种共10株从GenBank中获得)海带之间的系统进化关...对遗传背景清晰且具有显著表型差异的海带6个栽培品系(CUL002,CUL860,CUL1170,CUL017,CUL018,CUL901)和长海带(L.longissia)的ITS区进行了PCR扩增和序列测定,并分析了8个种17个品系(其中7种共10株从GenBank中获得)海带之间的系统进化关系.结果表明:表型各异的6个海带栽培品系ITS区差别很小,其中ITS1+5.8 S rDNA序列完全相同,部分品系间ITS2序列有个别碱基差异;进化树显示与日本海带(L.japonica,AF319018)聚在一起,相似性大于98%,其中CUL901、CUL860和CUL1170的ITS序列完全相同.长海带(L. longissia)的ITS+5.8 S rDNA和日本海带(L.japonica,AF319018)的完全相同.以Undaria peterseniana为外类群,根据ITS+5.8 S rDNA序列构建进化树,6栽培品系及长海带与日本海带聚在一起;17株海带基本构成两个大的进化枝.研究结果揭示了我国海域栽培的长海带可能与日本海带(L.japonica,AF319018)为同一个种.展开更多
Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG A...Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five展开更多
文摘对遗传背景清晰且具有显著表型差异的海带6个栽培品系(CUL002,CUL860,CUL1170,CUL017,CUL018,CUL901)和长海带(L.longissia)的ITS区进行了PCR扩增和序列测定,并分析了8个种17个品系(其中7种共10株从GenBank中获得)海带之间的系统进化关系.结果表明:表型各异的6个海带栽培品系ITS区差别很小,其中ITS1+5.8 S rDNA序列完全相同,部分品系间ITS2序列有个别碱基差异;进化树显示与日本海带(L.japonica,AF319018)聚在一起,相似性大于98%,其中CUL901、CUL860和CUL1170的ITS序列完全相同.长海带(L. longissia)的ITS+5.8 S rDNA和日本海带(L.japonica,AF319018)的完全相同.以Undaria peterseniana为外类群,根据ITS+5.8 S rDNA序列构建进化树,6栽培品系及长海带与日本海带聚在一起;17株海带基本构成两个大的进化枝.研究结果揭示了我国海域栽培的长海带可能与日本海带(L.japonica,AF319018)为同一个种.
文摘Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five
