何首乌饮对衰老大鼠脑组织细胞Rb／p53表达的影响

目的检测Rb、p53在衰老大鼠脑组织中的表达，探讨何首乌饮抗大鼠脑组织衰老机制。方法选用8周龄sD大鼠84只，体重180-220克，随机分为正常组、模型组、何首乌饮高、中、低剂量组、何首乌丸剂组、自然恢复组，每组12只，采用D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型。采用免疫组织化学、Westernblotting和RT—PCR法检测pKb、p53在大鼠脑组织的表达差异。结果模型组大鼠脑组织Rb、p53表达明显增加，pRb表达明显减少（P〈0．05）；何首乌饮、何首乌丸组能显著提高pR_b的表达，降低Rb、p53表达（P〈0．05），何首乌饮高剂量组调节作用大于其他各组（P〈0．05）。结论何首鸟饮通过调节脑组织Kb／p53表达起到延缓衰老的作用。

Expression of PEPT2 mRNA in the lung of rat with bleomycin-induced pulmonary fibrosis

Objective：Pulmonary fibrosis is a common pathological phenomena in lung cancer patients after chemotherapy or radiotherapy, which is a key factor hindering to transport ion of high concentrated drug to the lung tissue, peptide trans-porter has become targets of the rational design of peptides and peptide drug. The purpose of the study is to investigate the expression of PEPT2 mRNA in the lung of rats with bleomycin （BLM）-induced pulmonary fibrosis. Methods：Fifty healthy adult Spragne-Dawley rats were randomized into five groups, the rats in BLM 7d, 14d and 28d groups were treated with a single instil ation of 5 mg/kg of BLM, to induced pulmonary fibrosis models. On days 7, 14 and 28, the animals were kil ed by exsan-guination respectively. Normal saline （NS） group were treated by NS, on days 14, the animals were kil ed by exsanguinations. Control group were untreated. The lung samples were processed for light microscopy and determined the hydroxyproline （HYP） concentration. The expression of PEPT2 mRNA were measured by RT-PCR. PEPT2 cDNA fragments were tested by dideoxy chain termination. Results：Compared with control and NS group, HYP levels increased on day 7 of BLM group, but there was no statistical significant dif erence （P〉0.05）. HYP levels markedly increased on days 14 and 28 of BLM group, there was statistical significant dif erence （P〈0.01）. The morphological study showed that col agenous fiber proliferated on days 14 and 28 of BLM group, especial y on day 28, formed pulmonary fibrosis. There were no significant changes of pulmo-nary PEPT2 mRNA expression at dif erent groups （P〉0.05）. Conclusion：The pulmonary fibrosis models of SD rats can be induced by a single instil ation of 5 mg/kg of bleomycin on 28d. There were no significant changes of PEPT2 mRNA expression in the lung of rats with bleomycin-induced pulmonary fibrosis.

Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats

Objective To investigate the expression of amyloid beta precursor-like protein 1 （APLP 1） gene on the transcription level in hippocampus of pilocarpine-induced epileptic rats. Methods Epileptic rats were developed by LiCl （3 mmol/kg, i.p.） approximately 20 h prior to pilocarpine （30 mg/kg, i.p.） administration. The 3＇ end partial sequence of rat APLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epileptic rats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR. Results The 3＇ end partial sequence of rat APLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation ofAPLP1 mRNA expression at 6 h after epilepsy initiation （P 〈 0.05）, and at 30 h, this down-regulation became more dramatic （P 〈 0.01）, which lasted till day 15 （P 〈 0.01）. Conclusion The 3＇ end ofAPLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epileptic rats.

Effect of electro-acupuncture on gene expression in heart of rats with stress-induced pre-hypertension based on gene chip technology

OBJECTIVE:To explore electro-acupuncture's(EA's)effect on gene expression in heart of rats with stress-induced pre-hypertension and try to reveal its biological mechanism based on gene chip technology.METHODS:Twenty-seven Wistar male rats were randomly divided into 3 groups.The stress-induced hypertensive rat model was prepared by electric foot-shocks combined with generated noise.Molding cycle lasted for 14 days and EA intervene was applied on rats in model + EA group during model preparation.Rat Gene 2.0 Sense Target Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) method.RESULTS:Compared with blank control group,390 genes were changed in model group;compared with model control group,330 genes were changed in model + EA group.Significance analysis of gene function showed that the differentially expressed genes are those involved in biological process,molecular function and cellular components.RT-PCR result of the screened key genes is consistent with that of gene chip test.CONCLUTION:EA could significantly lower blood pressure of stress-induced pre-hypertension rats and affect its gene expression profile in heart.Genes that related to the contraction of vascular smooth muscle may be involved in EA's anti-hypertensive mechanism.