Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains...Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains unconfirmed.In our study,gene expression data of ESCC and clinical information from public databases were downloaded.Through LASSO-Cox regression analysis,a risk score(RS)signature map of prognosis was constructed and performed external verification with the GSE53625 cohort.The ESTIMATE,xCell,CIBERSORT,TIMER,and ImmuCellAI algorithms were employed to analyze infiltrating immune cells and generate an immune microenvironment(IM).Afterward,functional enrichment analysis clarified the underlying mechanism of the model.Nomogram was utilized for forecasting the survival rate of individual ESCC cases.As a result,we successfully constructed an OS-related genes(OSRGs)model and found that the survival rate of high-risk groups was lower than that of low-risk groups.The AUC of the ROC verified the strong prediction performance of the signal in these two cohorts further.According to independent prognostic analysis,the RS was identified as an independent risk factor for ESCC.The nomogram and follow-up data revealed that the RS possesses favorable predictive value for the prognosis of ESCC patients.qRT-PCR detection demonstrated increased expression of MPC1,COX6C,CYB5R3,CASP7,and CYCS in esophageal cancer patients.In conclusion,we have constructed an OSRGs model for ESCC to predict patients’prognosis,offering a novel insight into the potential application of the OSRGs model in ESCC.展开更多
针对基于Linux和TCG软件栈(Trusted computing group Software Stack,TSS)的复杂性问题,提出一种轻量级的可信软件栈。分析了TSS的基本结构与TSS在嵌入式系统的局限,总结出基于嵌入式系统的可信软件栈设计需求,设计出软件栈命令调用的...针对基于Linux和TCG软件栈(Trusted computing group Software Stack,TSS)的复杂性问题,提出一种轻量级的可信软件栈。分析了TSS的基本结构与TSS在嵌入式系统的局限,总结出基于嵌入式系统的可信软件栈设计需求,设计出软件栈命令调用的机制和软件栈的结构。此外,分析了TSS密钥管理缓存算法,在flash中定义一块密钥槽空间,方便密钥管理中直接访问,阐述密钥生成的逻辑过程,实现面向嵌入式系统的可信软件系统。经实验验证,该软件栈可以结合RT-Thread实时系统实现基本的可信计算功能。展开更多
基金Natural Science Foundation of Ningbo(Grant No.2021J261).
文摘Oxidative stress(OS)is intimately associated with tumorigenesis and has been considered a potential therapeutic strategy.However,the OS-associated therapeutic target for esophageal squamous cell carcinoma(ESCC)remains unconfirmed.In our study,gene expression data of ESCC and clinical information from public databases were downloaded.Through LASSO-Cox regression analysis,a risk score(RS)signature map of prognosis was constructed and performed external verification with the GSE53625 cohort.The ESTIMATE,xCell,CIBERSORT,TIMER,and ImmuCellAI algorithms were employed to analyze infiltrating immune cells and generate an immune microenvironment(IM).Afterward,functional enrichment analysis clarified the underlying mechanism of the model.Nomogram was utilized for forecasting the survival rate of individual ESCC cases.As a result,we successfully constructed an OS-related genes(OSRGs)model and found that the survival rate of high-risk groups was lower than that of low-risk groups.The AUC of the ROC verified the strong prediction performance of the signal in these two cohorts further.According to independent prognostic analysis,the RS was identified as an independent risk factor for ESCC.The nomogram and follow-up data revealed that the RS possesses favorable predictive value for the prognosis of ESCC patients.qRT-PCR detection demonstrated increased expression of MPC1,COX6C,CYB5R3,CASP7,and CYCS in esophageal cancer patients.In conclusion,we have constructed an OSRGs model for ESCC to predict patients’prognosis,offering a novel insight into the potential application of the OSRGs model in ESCC.
文摘针对基于Linux和TCG软件栈(Trusted computing group Software Stack,TSS)的复杂性问题,提出一种轻量级的可信软件栈。分析了TSS的基本结构与TSS在嵌入式系统的局限,总结出基于嵌入式系统的可信软件栈设计需求,设计出软件栈命令调用的机制和软件栈的结构。此外,分析了TSS密钥管理缓存算法,在flash中定义一块密钥槽空间,方便密钥管理中直接访问,阐述密钥生成的逻辑过程,实现面向嵌入式系统的可信软件系统。经实验验证,该软件栈可以结合RT-Thread实时系统实现基本的可信计算功能。
