AIM： To elucidate the role of Rab23 in hepatocellular carcinoma （HCC） by assessing the expression of Rab23 in HCC tissue and in HCC cell lines. METHODS： Primary tumors （n = 100） were stained with Rab23 antibodie...AIM： To elucidate the role of Rab23 in hepatocellular carcinoma （HCC） by assessing the expression of Rab23 in HCC tissue and in HCC cell lines. METHODS： Primary tumors （n = 100） were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of doublestranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescenceactivated cell sorting. RESULTS： High cytoplasmic and nuclear expression of Rab23 was found in 38 of 71 （53.5%） and in 49 of 68 HCC patients （72%） respectively, which correlated with tumor size. HCC cell lines expressed Rab23. In Hep3B cells, siRNA for Rab23 decreased Rab23 mRNA by 4.5-fold and protein expression by 2-fold. Survival rates at 24 and 48 h for Hep-3B cells tTansfected with siRNA were lower and about 30% Hep-3B cells were apoptotic. Knocking down rab23 suppressed Hep3B cell growth, suggesting that rab23 could play an important role in Hep3B cell growth. CONCLUSION： Rab23 is overexpressed and/or activatedin HCC. Rab23 may be both a HCC predictor and a target for treating HCC.展开更多
文摘目的探讨过表达和敲除Rab23对人乳腺癌Bcap-37细胞侵袭迁移能力的影响。方法应用Western blot法检测乳腺细胞HBL-100及乳腺癌细胞Bcap-37、MDA-MB-231、MCF-7中Rab23的表达情况。慢病毒转染乳腺癌细胞Bcap-37,筛选出稳定过表达和敲除Rab23的Bcap-37细胞。划痕愈合试验、Transwell实验检测过表达和敲除Rab23后Bcap-37细胞侵袭迁移能力的变化。结果 Western blot法结果显示,Rab23在乳腺和乳腺癌细胞系中均有表达,且Bcap-37细胞表达水平最高,与HBL-100细胞相比,差异有统计学意义(P<0.01);与空载体Bcap-37细胞比较,过表达Rab23的Bcap-37细胞侵袭迁移能力明显增强(P<0.01);敲除Rab23的Bcap-37细胞侵袭迁移能力明显减弱(P<0.01)。结论 Rab23在乳腺癌细胞Bcap-37的侵袭迁移中发挥着重要作用。
文摘AIM： To elucidate the role of Rab23 in hepatocellular carcinoma （HCC） by assessing the expression of Rab23 in HCC tissue and in HCC cell lines. METHODS： Primary tumors （n = 100） were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of doublestranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescenceactivated cell sorting. RESULTS： High cytoplasmic and nuclear expression of Rab23 was found in 38 of 71 （53.5%） and in 49 of 68 HCC patients （72%） respectively, which correlated with tumor size. HCC cell lines expressed Rab23. In Hep3B cells, siRNA for Rab23 decreased Rab23 mRNA by 4.5-fold and protein expression by 2-fold. Survival rates at 24 and 48 h for Hep-3B cells tTansfected with siRNA were lower and about 30% Hep-3B cells were apoptotic. Knocking down rab23 suppressed Hep3B cell growth, suggesting that rab23 could play an important role in Hep3B cell growth. CONCLUSION： Rab23 is overexpressed and/or activatedin HCC. Rab23 may be both a HCC predictor and a target for treating HCC.
